OCA at 05:54, 20 June 2018

OCA — Wed, 20 Jun 2018 05:54:30 GMT

←Older revision		Revision as of 05:54, 20 June 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6f4i is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of Drosophila melanogaster SNF==
		+	<StructureSection load='6f4i' size='340' side='right' caption='[[6f4i]], [[Resolution\|resolution]] 1.49Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6f4i]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F4I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F4I FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f4i OCA], [http://pdbe.org/6f4i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f4i RCSB], [http://www.ebi.ac.uk/pdbsum/6f4i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f4i ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SNRPA_DROME SNRPA_DROME]] Binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP (By similarity). Plays a role in regulating sex-lethal splicing.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The first RNA recognition motif of the Drosophila SNF protein is an example of an RNA binding protein with multi-specificity. It binds different RNA hairpin loops in spliceosomal U1 or U2 small nuclear RNAs, and only in the latter case requires the auxiliary U2A' protein. Here we investigate its functions by crystal structures of SNF alone and bound to U1 stem-loop II, U2A' or U2 stem-loop IV and U2A', SNF dynamics from NMR spectroscopy, and structure-guided mutagenesis in binding studies. We find that different loop-closing base pairs and a nucleotide exchange at the tips of the loops contribute to differential SNF affinity for the RNAs. U2A' immobilizes SNF and RNA residues to restore U2 stem-loop IV binding affinity, while U1 stem-loop II binding does not require such adjustments. Our findings show how U2A' can modulate RNA specificity of SNF without changing SNF conformation or relying on direct RNA contacts.

-	~~Authors~~:	+	Molecular principles underlying dual RNA specificity in the Drosophila SNF protein.,Weber G, DeKoster GT, Holton N, Hall KB, Wahl MC Nat Commun. 2018 Jun 7;9(1):2220. doi: 10.1038/s41467-018-04561-6. PMID:29880797<ref>PMID:29880797</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6f4i" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: DeKoster, G]]
		+	[[Category: Hall, K B]]
		+	[[Category: Holton, N]]
		+	[[Category: Wahl, M C]]
		+	[[Category: Weber, G]]
		+	[[Category: Evolution]]
		+	[[Category: Snf]]
		+	[[Category: Splicing]]
		+	[[Category: U2a'. rrm]]

OCA at 07:16, 10 January 2018

OCA — Wed, 10 Jan 2018 07:16:20 GMT

←Older revision		Revision as of 07:16, 10 January 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6f4i is ON HOLD	+	The entry 6f4i is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6f4i" [edit=sysop:move=sysop]

OCA — Wed, 06 Dec 2017 06:17:22 GMT

Protected "6f4i" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:17, 6 December 2017

OCA: New page: '''Unreleased structure''' The entry 6f4i is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 06 Dec 2017 06:17:21 GMT

New page: '''Unreleased structure''' The entry 6f4i is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6f4i is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6f4i - Revision history

OCA at 05:54, 20 June 2018

OCA at 07:16, 10 January 2018

OCA: Protected "6f4i" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6f4i is ON HOLD Authors: Description: Category: Unreleased Structures