6f6g - Revision history

OCA at 06:17, 11 July 2018

OCA — Wed, 11 Jul 2018 06:17:38 GMT

←Older revision		Revision as of 06:17, 11 July 2018
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	<StructureSection load='6f6g' size='340' side='right' caption='[[6f6g]], [[Resolution\|resolution]] 1.99Å' scene=''>		<StructureSection load='6f6g' size='340' side='right' caption='[[6f6g]], [[Resolution\|resolution]] 1.99Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6f6g]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F6G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F6G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6f6g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Geoka Geoka]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F6G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F6G FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hr0\|4hr0]], [[4hr4\|4hr4]], [[4hr5\|4hr5]], [[4xb9\|4xb9]], [[4xbv\|4xbv]], [[4xbw\|4xbw]], [[5dco\|5dco]], [[5dcr\|5dcr]], [[5dcs\|5dcs]], [[5ekb\|5ekb]], [[5omk\|5omk]], [[5omj\|5omj]], [[6f65\|6f65]], [[6f6b\|6f6b]], [[6f6c\|6f6c]], [[6f6e\|6f6e]], [[6f6f\|6f6f]], [[6f6h\|6f6h]], [[6f6k\|6f6k]], [[6f6l\|6f6l]], [[6f6m\|6f6m]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hr0\|4hr0]], [[4hr4\|4hr4]], [[4hr5\|4hr5]], [[4xb9\|4xb9]], [[4xbv\|4xbv]], [[4xbw\|4xbw]], [[5dco\|5dco]], [[5dcr\|5dcr]], [[5dcs\|5dcs]], [[5ekb\|5ekb]], [[5omk\|5omk]], [[5omj\|5omj]], [[6f65\|6f65]], [[6f6b\|6f6b]], [[6f6c\|6f6c]], [[6f6e\|6f6e]], [[6f6f\|6f6f]], [[6f6h\|6f6h]], [[6f6k\|6f6k]], [[6f6l\|6f6l]], [[6f6m\|6f6m]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GK2771 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=235909 GEOKA])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonucleoside-diphosphate_reductase Ribonucleoside-diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.4.1 1.17.4.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonucleoside-diphosphate_reductase Ribonucleoside-diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.4.1 1.17.4.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f6g OCA], [http://pdbe.org/6f6g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f6g RCSB], [http://www.ebi.ac.uk/pdbsum/6f6g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f6g ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f6g OCA], [http://pdbe.org/6f6g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f6g RCSB], [http://www.ebi.ac.uk/pdbsum/6f6g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f6g ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	R2-like ligand-binding oxidases contain a dinuclear metal cofactor which can consist either of two iron ions or one manganese and one iron ion, but the heterodinuclear Mn/Fe cofactor is the preferred assembly in the presence of Mn(II) and Fe(II) in vitro. We have previously shown that both types of cofactor are capable of catalyzing formation of a tyrosine-valine ether cross-link in the protein scaffold. Here we demonstrate that Mn/Fe centers catalyze cross-link formation more efficiently than Fe/Fe centers, indicating that the heterodinuclear cofactor is the biologically relevant one. We further explore the chemical potential of the Mn/Fe cofactor by introducing mutations at the cross-linking valine residue. We find that cross-link formation is possible also to the tertiary beta-carbon in an isoleucine, but not to the secondary beta-carbon or tertiary gamma-carbon in a leucine, nor to the primary beta-carbon of an alanine. These results illustrate that the reactivity of the cofactor is highly specific and directed.
		+
		+	Ether cross-link formation in the R2-like ligand-binding oxidase.,Griese JJ, Branca RMM, Srinivas V, Hogbom M J Biol Inorg Chem. 2018 Jun 26. pii: 10.1007/s00775-018-1583-3. doi:, 10.1007/s00775-018-1583-3. PMID:29946980<ref>PMID:29946980</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6f6g" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Geoka]]
	[[Category: Ribonucleoside-diphosphate reductase]]		[[Category: Ribonucleoside-diphosphate reductase]]
	[[Category: Griese, J J]]		[[Category: Griese, J J]]

OCA at 07:19, 4 July 2018

OCA — Wed, 04 Jul 2018 07:19:34 GMT

←Older revision		Revision as of 07:19, 4 July 2018
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-	'''Unreleased structure'''

-	~~The entry 6f6g is ON HOLD until Paper Publication~~	+	==R2-like ligand-binding oxidase V72I mutant with anaerobically reconstituted Mn/Fe cofactor==
-		+	<StructureSection load='6f6g' size='340' side='right' caption='[[6f6g]], [[Resolution\|resolution]] 1.99Å' scene=''>
-	~~Authors: Griese, J.J., Hogbom, M.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6f6g]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F6G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F6G FirstGlance]. <br>
-	~~Description:~~ R2-like ligand-binding oxidase V72I mutant with anaerobically reconstituted Mn/Fe cofactor	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hr0\|4hr0]], [[4hr4\|4hr4]], [[4hr5\|4hr5]], [[4xb9\|4xb9]], [[4xbv\|4xbv]], [[4xbw\|4xbw]], [[5dco\|5dco]], [[5dcr\|5dcr]], [[5dcs\|5dcs]], [[5ekb\|5ekb]], [[5omk\|5omk]], [[5omj\|5omj]], [[6f65\|6f65]], [[6f6b\|6f6b]], [[6f6c\|6f6c]], [[6f6e\|6f6e]], [[6f6f\|6f6f]], [[6f6h\|6f6h]], [[6f6k\|6f6k]], [[6f6l\|6f6l]], [[6f6m\|6f6m]]</td></tr>
-	[[Category: Griese, J.J]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonucleoside-diphosphate_reductase Ribonucleoside-diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.4.1 1.17.4.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f6g OCA], [http://pdbe.org/6f6g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f6g RCSB], [http://www.ebi.ac.uk/pdbsum/6f6g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f6g ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Ribonucleoside-diphosphate reductase]]
		+	[[Category: Griese, J J]]
	[[Category: Hogbom, M]]		[[Category: Hogbom, M]]
		+	[[Category: Metalloprotein oxidoreductase]]
		+	[[Category: Mn/fe cofactor]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: R2-like ligand-binding oxidase]]
		+	[[Category: Ribonucleotide reductase r2 subunit fold]]

OCA: Protected "6f6g" [edit=sysop:move=sysop]

OCA — Wed, 13 Dec 2017 06:02:27 GMT

Protected "6f6g" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:02, 13 December 2017

OCA: New page: '''Unreleased structure''' The entry 6f6g is ON HOLD until Paper Publication Authors: Griese, J.J., Hogbom, M. Description: R2-like ligand-binding oxidase V72I mutant with anaerobicall...

OCA — Wed, 13 Dec 2017 06:02:26 GMT

New page: '''Unreleased structure''' The entry 6f6g is ON HOLD until Paper Publication Authors: Griese, J.J., Hogbom, M. Description: R2-like ligand-binding oxidase V72I mutant with anaerobicall...

New page

'''Unreleased structure'''

The entry 6f6g is ON HOLD until Paper Publication

Authors: Griese, J.J., Hogbom, M.

Description: R2-like ligand-binding oxidase V72I mutant with anaerobically reconstituted Mn/Fe cofactor
[[Category: Unreleased Structures]]
[[Category: Griese, J.J]]
[[Category: Hogbom, M]]