6f9g - Revision history

OCA at 07:50, 23 May 2018

OCA — Wed, 23 May 2018 07:50:10 GMT

←Older revision		Revision as of 07:50, 23 May 2018
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	<StructureSection load='6f9g' size='340' side='right' caption='[[6f9g]], [[Resolution\|resolution]] 2.39Å' scene=''>		<StructureSection load='6f9g' size='340' side='right' caption='[[6f9g]], [[Resolution\|resolution]] 2.39Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6f9g]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F9G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6f9g]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Psepk Psepk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F9G FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PUT:1,4-DIAMINOBUTANE'>PUT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PUT:1,4-DIAMINOBUTANE'>PUT</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mcpU, PP_1228 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160488 PSEPK])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f9g OCA], [http://pdbe.org/6f9g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f9g RCSB], [http://www.ebi.ac.uk/pdbsum/6f9g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f9g ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f9g OCA], [http://pdbe.org/6f9g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f9g RCSB], [http://www.ebi.ac.uk/pdbsum/6f9g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f9g ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/MCPU_PSEPK MCPU_PSEPK]] Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce a signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of methylation. McpU is a chemoreceptor that specifically recognizes polyamines such as putrescine, spermidine and cadaverine.<ref>PMID:26662997</ref>		[[http://www.uniprot.org/uniprot/MCPU_PSEPK MCPU_PSEPK]] Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce a signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of methylation. McpU is a chemoreceptor that specifically recognizes polyamines such as putrescine, spermidine and cadaverine.<ref>PMID:26662997</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Many bacteria can move chemotactically to a variety of compounds and the recognition of chemoeffectors by the chemoreceptor ligand binding domain (LBD) defines the specificity of response. Many chemoreceptors were found to recognize different amino- and organic acids, but the McpU chemoreceptor from Pseudomonas putida was identified as the first chemoreceptor that bound specifically polyamines. We report here the three-dimensional structure of McpU-LBD in complex with putrescine at a resolution of 2.4 A, which fitted well a solution structure generated by Small Angle X-ray Scattering. Putrescine bound to a negatively charged pocket in the membrane distal module of McpU-LBD. Similarities exist in the binding of putrescine to McpU-LBD and taurine to the LBD of the Mlp37 chemoreceptor of Vibrio cholerae. In both structures the primary amino group of the respective ligand is recognized by hydrogen bonds established by two aspartate and a tyrosine side chain. This feature may be used to predict the ligands of chemoreceptors with unknown function. Analytical ultracentrifugation revealed that McpU-LBD is monomeric in solution and that ligand binding does not alter this oligomeric state. This sensing mode thus differs from that of the well-characterised four-helix bundle domains where ligands bind to two sites at the LBD dimer interface. Although there appear to be different sensing modes, results are discussed in the context of data indicating that chemoreceptors employ the same mechanism of transmembrane signaling. This work enhances our understanding of CACHE domains, which are the most abundant sensor domains in bacterial chemoreceptors and sensor kinases.
		+
		+	Structural basis for polyamine binding at the dCACHE domain of the McpU chemoreceptor from Pseudomonas putida.,Gavira JA, Ortega A, Martin-Mora D, Conejero-Muriel MT, Corral-Lugo A, Morel B, Matilla MA, Krell T J Mol Biol. 2018 May 11. pii: S0022-2836(18)30403-0. doi:, 10.1016/j.jmb.2018.05.008. PMID:29758259<ref>PMID:29758259</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6f9g" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Chemotaxis protein\|Chemotaxis protein]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Psepk]]
	[[Category: Conejero-Muriel, M T]]		[[Category: Conejero-Muriel, M T]]
	[[Category: Corral-Lugo, A]]		[[Category: Corral-Lugo, A]]

OCA at 06:41, 28 March 2018

OCA — Wed, 28 Mar 2018 06:41:54 GMT

←Older revision		Revision as of 06:41, 28 March 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6f9g ~~is ON HOLD until Paper Publication~~	+	==Ligand binding domain of P. putida KT2440 polyamine chemorecpetors McpU in complex putrescine.==
-		+	<StructureSection load='6f9g' size='340' side='right' caption='[[6f9g]], [[Resolution\|resolution]] 2.39Å' scene=''>
-	~~Authors~~: ~~Gavira, J~~.A.~~, Conejero~~-~~Muriel, M~~.T., ~~Ortega~~, A., ~~Martin~~-~~Mora, D~~., ~~Corral~~-~~Lugo~~, A., ~~Morel~~, B., ~~Krell, T~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6f9g]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F9G FirstGlance]. <br>
-	~~Description~~: ~~Ligand binding domain~~ of P. ~~putida KT2440 polyamine chemorecpetors~~ McpU ~~in complex~~ putrescine.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PUT:1,4-DIAMINOBUTANE'>PUT</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f9g OCA], [http://pdbe.org/6f9g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f9g RCSB], [http://www.ebi.ac.uk/pdbsum/6f9g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f9g ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MCPU_PSEPK MCPU_PSEPK]] Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce a signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of methylation. McpU is a chemoreceptor that specifically recognizes polyamines such as putrescine, spermidine and cadaverine.<ref>PMID:26662997</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Conejero-Muriel, M T]]
	[[Category: Corral-Lugo, A]]		[[Category: Corral-Lugo, A]]
-	[[Category: ~~Conejero-Muriel~~, M.T]]	+	[[Category: Gavira, J A]]
-	[[Category: ~~Gavira~~, ~~J.A~~]]	+	[[Category: Krell, T]]
		+	[[Category: Martin-Mora, D]]
	[[Category: Morel, B]]		[[Category: Morel, B]]
	[[Category: Ortega, A]]		[[Category: Ortega, A]]
-	[[Category: ~~Martin-Mora, D~~]]	+	[[Category: Chemotactic transducer]]
-	[[Category: ~~Krell, T~~]]	+	[[Category: Ligand binding domain]]
		+	[[Category: Signaling protein]]

OCA at 07:27, 27 December 2017

OCA — Wed, 27 Dec 2017 07:27:42 GMT

←Older revision		Revision as of 07:27, 27 December 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6f9g is ON HOLD	+	The entry 6f9g is ON HOLD until Paper Publication

	Authors: Gavira, J.A., Conejero-Muriel, M.T., Ortega, A., Martin-Mora, D., Corral-Lugo, A., Morel, B., Krell, T.		Authors: Gavira, J.A., Conejero-Muriel, M.T., Ortega, A., Martin-Mora, D., Corral-Lugo, A., Morel, B., Krell, T.

OCA: Protected "6f9g" [edit=sysop:move=sysop]

OCA — Wed, 20 Dec 2017 05:14:30 GMT

Protected "6f9g" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:14, 20 December 2017

OCA: New page: '''Unreleased structure''' The entry 6f9g is ON HOLD Authors: Gavira, J.A., Conejero-Muriel, M.T., Ortega, A., Martin-Mora, D., Corral-Lugo, A., Morel, B., Krell, T. Description: Ligan...

OCA — Wed, 20 Dec 2017 05:14:28 GMT

New page: '''Unreleased structure''' The entry 6f9g is ON HOLD Authors: Gavira, J.A., Conejero-Muriel, M.T., Ortega, A., Martin-Mora, D., Corral-Lugo, A., Morel, B., Krell, T. Description: Ligan...

New page

'''Unreleased structure'''

The entry 6f9g is ON HOLD

Authors: Gavira, J.A., Conejero-Muriel, M.T., Ortega, A., Martin-Mora, D., Corral-Lugo, A., Morel, B., Krell, T.

Description: Ligand binding domain of P. putida KT2440 polyamine chemorecpetors McpU in complex putrescine.
[[Category: Unreleased Structures]]
[[Category: Corral-Lugo, A]]
[[Category: Conejero-Muriel, M.T]]
[[Category: Gavira, J.A]]
[[Category: Morel, B]]
[[Category: Ortega, A]]
[[Category: Martin-Mora, D]]
[[Category: Krell, T]]