6fam - Revision history

OCA at 08:14, 14 June 2018

OCA — Thu, 14 Jun 2018 08:14:35 GMT

←Older revision		Revision as of 08:14, 14 June 2018
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	<StructureSection load='6fam' size='340' side='right' caption='[[6fam]], [[Resolution\|resolution]] 1.13Å' scene=''>		<StructureSection load='6fam' size='340' side='right' caption='[[6fam]], [[Resolution\|resolution]] 1.13Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6fam]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FAM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FAM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6fam]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteroides_sp._xb1a Bacteroides sp. xb1a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FAM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FAM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=G63:mannose-alpha-1,3-2-aminodeoxymannojirimycin'>G63</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=G63:mannose-alpha-1,3-2-aminodeoxymannojirimycin'>G63</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BXY_34140 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=657309 Bacteroides sp. XB1A])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fam OCA], [http://pdbe.org/6fam PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fam RCSB], [http://www.ebi.ac.uk/pdbsum/6fam PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fam ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fam OCA], [http://pdbe.org/6fam PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fam RCSB], [http://www.ebi.ac.uk/pdbsum/6fam PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fam ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacteroides sp. xb1a]]
	[[Category: Davies, G J]]		[[Category: Davies, G J]]
	[[Category: Fernandes, P Z]]		[[Category: Fernandes, P Z]]

OCA at 07:43, 21 March 2018

OCA — Wed, 21 Mar 2018 07:43:40 GMT

←Older revision		Revision as of 07:43, 21 March 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6fam is ~~ON HOLD until Paper~~ Publication	+	==Structure of the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex with mannose-alpha-1,3-2-aminodeoxymannojirimycin==
		+	<StructureSection load='6fam' size='340' side='right' caption='[[6fam]], [[Resolution\|resolution]] 1.13Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6fam]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FAM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FAM FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=G63:mannose-alpha-1,3-2-aminodeoxymannojirimycin'>G63</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fam OCA], [http://pdbe.org/6fam PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fam RCSB], [http://www.ebi.ac.uk/pdbsum/6fam PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fam ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Endo-alpha-1,2-mannosidases and -mannanases, members of glycoside hydrolase family 99 (GH99), cleave alpha-Glc/Man-1,3-alpha-Man-OR structures within mammalian N-linked glycans and fungal alpha-mannan, respectively. They are proposed to act through a two-step mechanism involving a 1,2-anhydrosugar 'epoxide' intermediate, involving two conserved catalytic residues. In the first step Glu333 acts as general base to deprotonate the 2-hydroxyl group adjacent to the fissile glycosidic bond, while Glu336 provides general acid assistance to departure of the aglycon. We report the synthesis of two inhibitors designed to interact with either the general base (alpha-mannosyl-1,3-(2-aminodeoxymannojirimycin); Man2NH2DMJ) or the general acid (alpha-mannosyl-1,3-mannoimidazole; ManManIm). Modest affinities were observed for an endo-alpha-1,2-mannanase from Bacteroides thetaiotaomicron. Structural studies reveal that Man2NH2DMJ binds like other iminosugar inhibitors, suggesting that the poor inhibition by this compound is not a result of a failure to achieve the expected interaction with the general base, but rather the reduction in basicity of the endocyclic nitrogen caused by introduction of a vicinal, protonated amine at C2. ManManIm binds with the imidazole headgroup distorted downwards, a result of an unfavourable interaction with a conserved active site tyrosine. This study identifies important limitations associated with mechanism-inspired inhibitor design for GH99 enzymes.

-	~~Authors~~:	+	Exploration of strategies for mechanism-based inhibitor design for family GH99 endo-alpha-1,2-mannanases.,Fernandes PZ, Petricevic M, Sobala L, Davies GJ, Williams SJ Chemistry. 2018 Mar 5. doi: 10.1002/chem.201800435. PMID:29508463<ref>PMID:29508463</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6fam" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Davies, G J]]
		+	[[Category: Fernandes, P Z]]
		+	[[Category: Petricevic, M]]
		+	[[Category: Sobala, L F]]
		+	[[Category: Williams, S J]]
		+	[[Category: Complex]]
		+	[[Category: Gh99]]
		+	[[Category: Hydrolase]]

OCA: Protected "6fam" [edit=sysop:move=sysop]

OCA — Wed, 27 Dec 2017 07:29:47 GMT

Protected "6fam" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:29, 27 December 2017

OCA: New page: '''Unreleased structure''' The entry 6fam is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Dec 2017 07:29:46 GMT

New page: '''Unreleased structure''' The entry 6fam is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6fam is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]