OCA at 08:18, 26 December 2018

OCA — Wed, 26 Dec 2018 08:18:58 GMT

←Older revision		Revision as of 08:18, 26 December 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6fcg is ~~ON HOLD~~	+	==Crystal structure of an endo-laminarinase from Formosa Hel1_33_131==
		+	<StructureSection load='6fcg' size='340' side='right' caption='[[6fcg]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6fcg]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FCG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FCG FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fcg OCA], [http://pdbe.org/6fcg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fcg RCSB], [http://www.ebi.ac.uk/pdbsum/6fcg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fcg ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Polysaccharide degradation by heterotrophic microbes is a key process within Earth's carbon cycle. Here, we use environmental proteomics and metagenomics in combination with cultivation experiments and biochemical characterizations to investigate the molecular details of in situ polysaccharide degradation mechanisms during microalgal blooms. For this, we use laminarin as a model polysaccharide. Laminarin is a ubiquitous marine storage polymer of marine microalgae and is particularly abundant during phytoplankton blooms. In this study, we show that highly specialized bacterial strains of the Bacteroidetes phylum repeatedly reached high abundances during North Sea algal blooms and dominated laminarin turnover. These genomically streamlined bacteria of the genus Formosa have an expanded set of laminarin hydrolases and transporters that belonged to the most abundant proteins in the environmental samples. In vitro experiments with cultured isolates allowed us to determine the functions of in situ expressed key enzymes and to confirm their role in laminarin utilization. It is shown that laminarin consumption of Formosa spp. is paralleled by enhanced uptake of diatom-derived peptides. This study reveals that genome reduction, enzyme fusions, transporters, and enzyme expansion as well as a tight coupling of carbon and nitrogen metabolism provide the tools, which make Formosa spp. so competitive during microalgal blooms.

-	~~Authors: Becker~~, S., Robb, C.S., ~~Hehemann~~, J.H.	+	Adaptive mechanisms that provide competitive advantages to marine bacteroidetes during microalgal blooms.,Unfried F, Becker S, Robb CS, Hehemann JH, Markert S, Heiden SE, Hinzke T, Becher D, Reintjes G, Kruger K, Avci B, Kappelmann L, Hahnke RL, Fischer T, Harder J, Teeling H, Fuchs B, Barbeyron T, Amann RI, Schweder T ISME J. 2018 Dec;12(12):2894-2906. doi: 10.1038/s41396-018-0243-5. Epub 2018 Jul , 30. PMID:30061707<ref>PMID:30061707</ref>

-	~~Description: Crystal structure~~ of ~~an endo-laminarinase from Formosa Hel1_33_131~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 6fcg" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Becker, S]]		[[Category: Becker, S]]
-	[[Category: Hehemann, J.H]]	+	[[Category: Hehemann, J H]]
-	[[Category: Robb, C.S]]	+	[[Category: Robb, C S]]
		+	[[Category: Bacteria]]
		+	[[Category: Gh17]]
		+	[[Category: Glycoside hydrolase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Laminarinase]]

OCA: Protected "6fcg" [edit=sysop:move=sysop]

OCA — Wed, 27 Dec 2017 07:34:15 GMT

Protected "6fcg" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:34, 27 December 2017

OCA: New page: '''Unreleased structure''' The entry 6fcg is ON HOLD Authors: Becker, S., Robb, C.S., Hehemann, J.H. Description: Crystal structure of an endo-laminarinase from Formosa Hel1_33_131 [[C...

OCA — Wed, 27 Dec 2017 07:34:14 GMT

New page: '''Unreleased structure''' The entry 6fcg is ON HOLD Authors: Becker, S., Robb, C.S., Hehemann, J.H. Description: Crystal structure of an endo-laminarinase from Formosa Hel1_33_131 [[C...

New page

'''Unreleased structure'''

The entry 6fcg is ON HOLD

Authors: Becker, S., Robb, C.S., Hehemann, J.H.

Description: Crystal structure of an endo-laminarinase from Formosa Hel1_33_131
[[Category: Unreleased Structures]]
[[Category: Becker, S]]
[[Category: Hehemann, J.H]]
[[Category: Robb, C.S]]

6fcg - Revision history

OCA at 08:18, 26 December 2018

OCA: Protected "6fcg" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6fcg is ON HOLD Authors: Becker, S., Robb, C.S., Hehemann, J.H. Description: Crystal structure of an endo-laminarinase from Formosa Hel1_33_131 [[C...