6fe4 - Revision history

OCA at 07:05, 14 March 2018

OCA — Wed, 14 Mar 2018 07:05:24 GMT

←Older revision		Revision as of 07:05, 14 March 2018
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	<StructureSection load='6fe4' size='340' side='right' caption='[[6fe4]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='6fe4' size='340' side='right' caption='[[6fe4]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6fe4]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FE4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FE4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6fe4]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Alpaca Alpaca] and [http://en.wikipedia.org/wiki/Bp933 Bp933]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FE4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FE4 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fe4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fe4 OCA], [http://pdbe.org/6fe4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fe4 RCSB], [http://www.ebi.ac.uk/pdbsum/6fe4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fe4 ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">stxB2, stx2B, L0104 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10730 BP933])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fe4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fe4 OCA], [http://pdbe.org/6fe4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fe4 RCSB], [http://www.ebi.ac.uk/pdbsum/6fe4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fe4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/STXB_BP933 STXB_BP933]] The B subunit is responsible for the binding of the holotoxin to specific receptors on the target cell surface, such as globotriaosylceramide (Gb3) in human intestinal microvilli.		[[http://www.uniprot.org/uniprot/STXB_BP933 STXB_BP933]] The B subunit is responsible for the binding of the holotoxin to specific receptors on the target cell surface, such as globotriaosylceramide (Gb3) in human intestinal microvilli.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: Shiga toxin-producing Escherichia coli (STEC) are a subset of pathogens leading to illnesses such as diarrhea, hemolytic uremic syndrome and even death. The Shiga toxins are the main virulence factors and divided in two groups: Stx1 and Stx2, of which the latter is more frequently associated with severe pathologies in humans. RESULTS: An immune library of nanobodies (Nbs) was constructed after immunizing an alpaca with recombinant Shiga toxin-2a B subunit (rStx2aB), to retrieve multiple rStx2aB-specific Nbs. The specificity of five Nbs towards rStx2aB was confirmed in ELISA and Western blot. Nb113 had the highest affinity (9.6 nM) and its bivalent construct exhibited a 100-fold higher functional affinity. The structure of the Nb113 in complex with rStx2aB was determined via X-ray crystallography. The crystal structure of the Nb113-rStx2aB complex revealed that five copies of Nb113 bind to the rStx2aB pentamer and that the Nb113 epitope overlaps with the Gb3 binding site, thereby providing a structural basis for the neutralization of Stx2a by Nb113 that was observed on Vero cells. Finally, the tandem-repeated, bivalent Nb113(2) exhibits a higher toxin neutralization capacity compared to monovalent Nb113. CONCLUSIONS: The Nb of highest affinity for rStx2aB is also the best Stx2a and Stx2c toxin neutralizing Nb, especially in a bivalent format. This lead Nb neutralizes Stx2a by competing for the Gb3 receptor. The fusion of the bivalent Nb113(2) with a serum albumin specific Nb is expected to combine high toxin neutralization potential with prolonged blood circulation.
		+
		+	Structural Basis for the Specific Neutralization of Stx2a with a Camelid Single Domain Antibody Fragment.,Bernedo-Navarro RA, Romao E, Yano T, Pinto J, De Greve H, Sterckx YG, Muyldermans S Toxins (Basel). 2018 Mar 1;10(3). pii: toxins10030108. doi:, 10.3390/toxins10030108. PMID:29494518<ref>PMID:29494518</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6fe4" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Alpaca]]
		+	[[Category: Bp933]]
	[[Category: Bernedo, R]]		[[Category: Bernedo, R]]
	[[Category: Muyldermans, S]]		[[Category: Muyldermans, S]]

OCA at 05:11, 8 March 2018

OCA — Thu, 08 Mar 2018 05:11:15 GMT

←Older revision		Revision as of 05:11, 8 March 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6fe4 ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of the complex between Shiga toxin Stx2 B subunit and neutralising Nb113==
-		+	<StructureSection load='6fe4' size='340' side='right' caption='[[6fe4]], [[Resolution\|resolution]] 3.00Å' scene=''>
-	~~Authors~~: ~~Bernedo~~, R., ~~Muyldermans, S~~., ~~Sterckx~~, Y.G.J.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6fe4]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FE4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FE4 FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of the complex between Shiga toxin Stx2~~ B subunit ~~and neutralising Nb113~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fe4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fe4 OCA], [http://pdbe.org/6fe4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fe4 RCSB], [http://www.ebi.ac.uk/pdbsum/6fe4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fe4 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/STXB_BP933 STXB_BP933]] The B subunit is responsible for the binding of the holotoxin to specific receptors on the target cell surface, such as globotriaosylceramide (Gb3) in human intestinal microvilli.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bernedo, R]]
	[[Category: Muyldermans, S]]		[[Category: Muyldermans, S]]
-	[[Category: Sterckx, Y.G.J]]	+	[[Category: Sterckx, Y G.J]]
-	[[Category: ~~Bernedo, R~~]]	+	[[Category: Complex]]
		+	[[Category: Nanobody]]
		+	[[Category: Shiga]]
		+	[[Category: Toxin]]

OCA at 07:17, 10 January 2018

OCA — Wed, 10 Jan 2018 07:17:48 GMT

←Older revision		Revision as of 07:17, 10 January 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6fe4 is ON HOLD	+	The entry 6fe4 is ON HOLD until Paper Publication

	Authors: Bernedo, R., Muyldermans, S., Sterckx, Y.G.J.		Authors: Bernedo, R., Muyldermans, S., Sterckx, Y.G.J.

OCA: Protected "6fe4" [edit=sysop:move=sysop]

OCA — Wed, 03 Jan 2018 04:58:56 GMT

Protected "6fe4" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:58, 3 January 2018

OCA: New page: '''Unreleased structure''' The entry 6fe4 is ON HOLD Authors: Bernedo, R., Muyldermans, S., Sterckx, Y.G.J. Description: Crystal structure of the complex between Shiga toxin Stx2 B sub...

OCA — Wed, 03 Jan 2018 04:58:55 GMT

New page: '''Unreleased structure''' The entry 6fe4 is ON HOLD Authors: Bernedo, R., Muyldermans, S., Sterckx, Y.G.J. Description: Crystal structure of the complex between Shiga toxin Stx2 B sub...

New page

'''Unreleased structure'''

The entry 6fe4 is ON HOLD

Authors: Bernedo, R., Muyldermans, S., Sterckx, Y.G.J.

Description: Crystal structure of the complex between Shiga toxin Stx2 B subunit and neutralising Nb113
[[Category: Unreleased Structures]]
[[Category: Muyldermans, S]]
[[Category: Sterckx, Y.G.J]]
[[Category: Bernedo, R]]