6fgk - Revision history

OCA at 07:10, 15 February 2018

OCA — Thu, 15 Feb 2018 07:10:44 GMT

←Older revision		Revision as of 07:10, 15 February 2018
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	<StructureSection load='6fgk' size='340' side='right' caption='[[6fgk]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='6fgk' size='340' side='right' caption='[[6fgk]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6fgk]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FGK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FGK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6fgk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FGK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FGK FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6fhj\|6fhj]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6fhj\|6fhj]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ywaC, BSU38480, ipa-7d ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/GTP_diphosphokinase GTP diphosphokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.6.5 2.7.6.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/GTP_diphosphokinase GTP diphosphokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.6.5 2.7.6.5] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fgk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fgk OCA], [http://pdbe.org/6fgk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fgk RCSB], [http://www.ebi.ac.uk/pdbsum/6fgk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fgk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fgk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fgk OCA], [http://pdbe.org/6fgk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fgk RCSB], [http://www.ebi.ac.uk/pdbsum/6fgk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fgk ProSAT]</span></td></tr>
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/YWAC_BACSU YWAC_BACSU]] Functions as a (p)ppGpp synthase; GDP can be used instead of GTP, resulting in an increase of (p)ppGpp synthesis (PubMed:18067544). Overexpression in relA mutants (triple relA-yjbM-ywaC deletions and single relA deletions) leads to growth arrest; GTP levels fall drastically, various guanine-related nucleotides are synthesized (ppGp or pGpp), the cellular transcriptional profile changes dramatically and 70S ribosome dimerization occurs (PubMed:22950019). Overexpression in the presence of a wild-type relA gene does not have these effects (PubMed:22950019). In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. activities in response to changes in nutritional abundance. YwaC has probably a minor role in stringent response (PubMed:18067544).<ref>PMID:18067544</ref> <ref>PMID:22950019</ref>		[[http://www.uniprot.org/uniprot/YWAC_BACSU YWAC_BACSU]] Functions as a (p)ppGpp synthase; GDP can be used instead of GTP, resulting in an increase of (p)ppGpp synthesis (PubMed:18067544). Overexpression in relA mutants (triple relA-yjbM-ywaC deletions and single relA deletions) leads to growth arrest; GTP levels fall drastically, various guanine-related nucleotides are synthesized (ppGp or pGpp), the cellular transcriptional profile changes dramatically and 70S ribosome dimerization occurs (PubMed:22950019). Overexpression in the presence of a wild-type relA gene does not have these effects (PubMed:22950019). In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. activities in response to changes in nutritional abundance. YwaC has probably a minor role in stringent response (PubMed:18067544).<ref>PMID:18067544</ref> <ref>PMID:22950019</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The nutritional alarmones ppGpp and pppGpp (collectively: (p)ppGpp) are nucleotide-based second messengers enabling bacteria to respond to environmental and stress conditions. Several bacterial species contain two highly homologous (p)ppGpp synthetases named RelP (SAS2, YwaC) and RelQ (SAS1, YjbM). It is established that RelQ forms homotetramers that are subject to positive allosteric regulation by pppGpp, but structural and mechanistic insights into RelP lack behind. Here we present a structural and mechanistic characterization of RelP. In stark contrast to RelQ, RelP is not allosterically regulated by pppGpp and displays a different enzyme kinetic behavior. This discrepancy is evoked by different conformational properties of the guanosine-substrate binding site (G-Loop) of both proteins. Our study shows how minor structural divergences between close homologues result in new functional features during the course of molecular evolution.
		+
		+	Structural and mechanistic divergence of the small (p)ppGpp synthetases RelP and RelQ.,Steinchen W, Vogt MS, Altegoer F, Giammarinaro PI, Horvatek P, Wolz C, Bange G Sci Rep. 2018 Feb 1;8(1):2195. doi: 10.1038/s41598-018-20634-4. PMID:29391580<ref>PMID:29391580</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6fgk" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacsu]]
	[[Category: GTP diphosphokinase]]		[[Category: GTP diphosphokinase]]
	[[Category: Altegoer, F]]		[[Category: Altegoer, F]]

OCA at 06:24, 7 February 2018

OCA — Wed, 07 Feb 2018 06:24:27 GMT

←Older revision		Revision as of 06:24, 7 February 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6fgk is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of the small alarmone synthethase 2 from Bacillus subtilis==
-		+	<StructureSection load='6fgk' size='340' side='right' caption='[[6fgk]], [[Resolution\|resolution]] 3.20Å' scene=''>
-	~~Authors~~: ~~Bange~~, G., ~~Vogt~~, M., ~~Steinchen~~, W., ~~Altegoer, F~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6fgk]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FGK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FGK FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure~~ of the ~~small alarmone synthethase 2 from Bacillus subtilis~~	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6fhj\|6fhj]]</td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/GTP_diphosphokinase GTP diphosphokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.6.5 2.7.6.5] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fgk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fgk OCA], [http://pdbe.org/6fgk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fgk RCSB], [http://www.ebi.ac.uk/pdbsum/6fgk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fgk ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/YWAC_BACSU YWAC_BACSU]] Functions as a (p)ppGpp synthase; GDP can be used instead of GTP, resulting in an increase of (p)ppGpp synthesis (PubMed:18067544). Overexpression in relA mutants (triple relA-yjbM-ywaC deletions and single relA deletions) leads to growth arrest; GTP levels fall drastically, various guanine-related nucleotides are synthesized (ppGp or pGpp), the cellular transcriptional profile changes dramatically and 70S ribosome dimerization occurs (PubMed:22950019). Overexpression in the presence of a wild-type relA gene does not have these effects (PubMed:22950019). In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. activities in response to changes in nutritional abundance. YwaC has probably a minor role in stringent response (PubMed:18067544).<ref>PMID:18067544</ref> <ref>PMID:22950019</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: GTP diphosphokinase]]
		+	[[Category: Altegoer, F]]
	[[Category: Bange, G]]		[[Category: Bange, G]]
-	[[Category: Altegoer, F]]
	[[Category: Steinchen, W]]		[[Category: Steinchen, W]]
	[[Category: Vogt, M]]		[[Category: Vogt, M]]
		+	[[Category: Alarmone]]
		+	[[Category: Relp]]
		+	[[Category: Sas2]]
		+	[[Category: Stringent response]]
		+	[[Category: Transferase]]

OCA: Protected "6fgk" [edit=sysop:move=sysop]

OCA — Wed, 17 Jan 2018 05:54:35 GMT

Protected "6fgk" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:54, 17 January 2018

OCA: New page: '''Unreleased structure''' The entry 6fgk is ON HOLD until Paper Publication Authors: Bange, G., Vogt, M., Steinchen, W., Altegoer, F. Description: Crystal structure of the small alarm...

OCA — Wed, 17 Jan 2018 05:54:34 GMT

New page: '''Unreleased structure''' The entry 6fgk is ON HOLD until Paper Publication Authors: Bange, G., Vogt, M., Steinchen, W., Altegoer, F. Description: Crystal structure of the small alarm...

New page

'''Unreleased structure'''

The entry 6fgk is ON HOLD until Paper Publication

Authors: Bange, G., Vogt, M., Steinchen, W., Altegoer, F.

Description: Crystal structure of the small alarmone synthethase 2 from Bacillus subtilis
[[Category: Unreleased Structures]]
[[Category: Bange, G]]
[[Category: Altegoer, F]]
[[Category: Steinchen, W]]
[[Category: Vogt, M]]