6fh8 - Revision history

OCA at 12:28, 9 May 2024

OCA — Thu, 09 May 2024 12:28:14 GMT

←Older revision		Revision as of 12:28, 9 May 2024
Line 1:		Line 1:

	==E. coli surface display of streptavidin for directed evolution of an allylic deallocase==		==E. coli surface display of streptavidin for directed evolution of an allylic deallocase==
-	<StructureSection load='6fh8' size='340' side='right' caption='[[6fh8]], [[Resolution\|resolution]] 1.64Å' scene=''>	+	<StructureSection load='6fh8' size='340' side='right'caption='[[6fh8]], [[Resolution\|resolution]] 1.64Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6fh8]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FH8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6FH8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6fh8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FH8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6FH8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=JCT:biotinylated+ruthenium+cyclopentadienide'>JCT</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.64Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6fh8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fh8 OCA], [~~http~~://pdbe.org/6fh8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6fh8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6fh8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6fh8 ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=JCT:biotinylated+ruthenium+cyclopentadienide'>JCT</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6fh8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fh8 OCA], [https://pdbe.org/6fh8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6fh8 RCSB], [https://www.ebi.ac.uk/pdbsum/6fh8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6fh8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).	+	[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 6fh8" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6fh8" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Avidin 3D structures\|Avidin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bernhardes, V Pinheiro~~]]	+	[[Category: Large Structures]]
-	[[Category: Csibra, E]]	+	[[Category: Streptomyces avidinii]]
-	[[Category: Garabedian, B]]	+	[[Category: Csibra E]]
-	[[Category: Heinisch, T]]	+	[[Category: Garabedian B]]
-	[[Category: Jeschek, M]]	+	[[Category: Heinisch T]]
-	[[Category: Marliere, P]]	+	[[Category: Jeschek M]]
-	[[Category: Panke, S]]	+	[[Category: Marliere P]]
-	[[Category: ~~Schwizer, F~~]]	+	[[Category: Panke S]]
-	[[Category: ~~Ward, T R~~]]	+	[[Category: Pinheiro Bernhardes V]]
-	[[Category: ~~Allyl deprotection]]~~	+	[[Category: Schwizer F]]
-	~~[[Category: Artificial metalloenzyme]]~~	+	[[Category: Ward TR]]
-	~~[[Category: Bioorthogonal reaction]]~~	+
-	~~[[Category: Biotin]]~~	+
-	~~[[Category: Biotin binding protein]]~~	+
-	~~[[Category: Directed evolution]]~~	+
-	~~[[Category: E. coli surface display]]~~	+
-	~~[[Category: Streptavidin]]~~	+
-	~~[[Category: Uncaging~~]]	+

OCA at 06:11, 22 August 2018

OCA — Wed, 22 Aug 2018 06:11:15 GMT

←Older revision		Revision as of 06:11, 22 August 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6fh8 is ~~ON HOLD~~	+	==E. coli surface display of streptavidin for directed evolution of an allylic deallocase==
		+	<StructureSection load='6fh8' size='340' side='right' caption='[[6fh8]], [[Resolution\|resolution]] 1.64Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6fh8]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FH8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FH8 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=JCT:biotinylated+ruthenium+cyclopentadienide'>JCT</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fh8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fh8 OCA], [http://pdbe.org/6fh8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fh8 RCSB], [http://www.ebi.ac.uk/pdbsum/6fh8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fh8 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Artificial metalloenzymes (ArMs hereafter) combine attractive features of both homogeneous catalysts and enzymes and offer the potential to implement new-to-nature reactions in living organisms. Herein we present an E. coli surface display platform for streptavidin (Sav hereafter) relying on an Lpp-OmpA anchor. The system was used for the high throughput screening of a bioorthogonal CpRu-based artificial deallylase (ADAse) that uncages an allylcarbamate-protected aminocoumarin 1. Two rounds of directed evolution afforded the double mutant S112M-K121A that displayed a 36-fold increase in surface activity vs. cellular background and a 5.7-fold increased in vitro activity compared to the wild type enzyme. The crystal structure of the best ADAse reveals the importance of mutation S112M to stabilize the cofactor conformation inside the protein.

-	~~Authors:~~ Heinisch, T., Schwizer, F., Garabedian, B., Csibra, E., Jeschek, M., Pinheiro ~~Bernhardes, V.~~, Marliere, P., Panke, S., Ward, ~~T.R~~.	+	E. coli surface display of streptavidin for directed evolution of an allylic deallylase.,Heinisch T, Schwizer F, Garabedian B, Csibra E, Jeschek M, Vallapurackal J, Pinheiro VB, Marliere P, Panke S, Ward TR Chem Sci. 2018 May 24;9(24):5383-5388. doi: 10.1039/c8sc00484f. eCollection 2018 , Jun 28. PMID:30079176<ref>PMID:30079176</ref>

-	~~Description: E~~. ~~coli surface display~~ of ~~streptavidin for directed evolution of an allylic deallocase~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Panke, S]]~~	+	<div class="pdbe-citations 6fh8" style="background-color:#fffaf0;"></div>
-	~~[[Category: Ward, T.R]]~~	+	== References ==
-	~~[[Category: Marliere, P]]~~	+	<references/>
-	[[Category: ~~Jeschek~~, M]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Bernhardes, V Pinheiro]]
	[[Category: Csibra, E]]		[[Category: Csibra, E]]
-	[[Category: Heinisch, T]]
	[[Category: Garabedian, B]]		[[Category: Garabedian, B]]
-	[[Category: ~~Pinheiro Bernhardes~~, V]]	+	[[Category: Heinisch, T]]
		+	[[Category: Jeschek, M]]
		+	[[Category: Marliere, P]]
		+	[[Category: Panke, S]]
	[[Category: Schwizer, F]]		[[Category: Schwizer, F]]
		+	[[Category: Ward, T R]]
		+	[[Category: Allyl deprotection]]
		+	[[Category: Artificial metalloenzyme]]
		+	[[Category: Bioorthogonal reaction]]
		+	[[Category: Biotin]]
		+	[[Category: Biotin binding protein]]
		+	[[Category: Directed evolution]]
		+	[[Category: E. coli surface display]]
		+	[[Category: Streptavidin]]
		+	[[Category: Uncaging]]

OCA: Protected "6fh8" [edit=sysop:move=sysop]

OCA — Wed, 24 Jan 2018 17:25:48 GMT

Protected "6fh8" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:25, 24 January 2018

OCA: New page: '''Unreleased structure''' The entry 6fh8 is ON HOLD Authors: Heinisch, T., Schwizer, F., Garabedian, B., Csibra, E., Jeschek, M., Pinheiro Bernhardes, V., Marliere, P., Panke, S., Ward...

OCA — Wed, 24 Jan 2018 17:25:47 GMT

New page: '''Unreleased structure''' The entry 6fh8 is ON HOLD Authors: Heinisch, T., Schwizer, F., Garabedian, B., Csibra, E., Jeschek, M., Pinheiro Bernhardes, V., Marliere, P., Panke, S., Ward...

New page

'''Unreleased structure'''

The entry 6fh8 is ON HOLD

Authors: Heinisch, T., Schwizer, F., Garabedian, B., Csibra, E., Jeschek, M., Pinheiro Bernhardes, V., Marliere, P., Panke, S., Ward, T.R.

Description: E. coli surface display of streptavidin for directed evolution of an allylic deallocase
[[Category: Unreleased Structures]]
[[Category: Panke, S]]
[[Category: Ward, T.R]]
[[Category: Marliere, P]]
[[Category: Jeschek, M]]
[[Category: Csibra, E]]
[[Category: Heinisch, T]]
[[Category: Garabedian, B]]
[[Category: Pinheiro Bernhardes, V]]
[[Category: Schwizer, F]]