6fhf - Revision history

OCA at 19:10, 1 August 2018

OCA — Wed, 01 Aug 2018 19:10:39 GMT

←Older revision		Revision as of 19:10, 1 August 2018
Line 3:		Line 3:
	<StructureSection load='6fhf' size='340' side='right' caption='[[6fhf]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='6fhf' size='340' side='right' caption='[[6fhf]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6fhf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FHF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FHF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6fhf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FHF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FHF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6fhe\|6fhe]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6fhe\|6fhe]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fhf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fhf OCA], [http://pdbe.org/6fhf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fhf RCSB], [http://www.ebi.ac.uk/pdbsum/6fhf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fhf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fhf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fhf OCA], [http://pdbe.org/6fhf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fhf RCSB], [http://www.ebi.ac.uk/pdbsum/6fhf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fhf ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Automated design of enzymes with wild-type-like catalytic properties has been a long-standing but elusive goal. Here, we present a general, automated method for enzyme design through combinatorial backbone assembly. Starting from a set of homologous yet structurally diverse enzyme structures, the method assembles new backbone combinations and uses Rosetta to optimize the amino acid sequence, while conserving key catalytic residues. We apply this method to two unrelated enzyme families with TIM-barrel folds, glycoside hydrolase 10 (GH10) xylanases and phosphotriesterase-like lactonases (PLLs), designing 43 and 34 proteins, respectively. Twenty-one GH10 and seven PLL designs are active, including designs derived from templates with <25% sequence identity. Moreover, four designs are as active as natural enzymes in these families. Atomic accuracy in a high-activity GH10 design is further confirmed by crystallographic analysis. Thus, combinatorial-backbone assembly and design may be used to generate stable, active, and structurally diverse enzymes with altered selectivity or activity.
		+
		+	Highly active enzymes by automated combinatorial backbone assembly and sequence design.,Lapidoth G, Khersonsky O, Lipsh R, Dym O, Albeck S, Rogotner S, Fleishman SJ Nat Commun. 2018 Jul 17;9(1):2780. doi: 10.1038/s41467-018-05205-5. PMID:30018322<ref>PMID:30018322</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6fhf" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
	[[Category: Albeck, S]]		[[Category: Albeck, S]]
	[[Category: Dym, O]]		[[Category: Dym, O]]

OCA at 07:21, 25 July 2018

OCA — Wed, 25 Jul 2018 07:21:59 GMT

←Older revision		Revision as of 07:21, 25 July 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6fhf is ~~ON HOLD~~	+	==Highly active enzymes by automated modular backbone assembly and sequence design==
-		+	<StructureSection load='6fhf' size='340' side='right' caption='[[6fhf]], [[Resolution\|resolution]] 1.85Å' scene=''>
-	~~Authors~~: ~~Lapidot~~, G.~~, Khersonsky, O~~., ~~Lipsh~~, R., ~~Dym, O~~., Albeck, S~~., Rogotner, S.~~, Fleishman, J.S.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6fhf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FHF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FHF FirstGlance]. <br>
-	~~Description: Highly active enzymes by automated modular backbone assembly and sequence design~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6fhe\|6fhe]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fhf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fhf OCA], [http://pdbe.org/6fhf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fhf RCSB], [http://www.ebi.ac.uk/pdbsum/6fhf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fhf ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Albeck, S]]
		+	[[Category: Dym, O]]
		+	[[Category: Fleishman, J S]]
		+	[[Category: Khersonsky, O]]
	[[Category: Lapidot, G]]		[[Category: Lapidot, G]]
-	[[Category: Albeck, S]]
	[[Category: Lipsh, R]]		[[Category: Lipsh, R]]
	[[Category: Rogotner, S]]		[[Category: Rogotner, S]]
-	[[Category: ~~Fleishman, J.S~~]]	+	[[Category: Artificial enzyme]]
-	[[Category: ~~Dym, O]]~~	+	[[Category: Design]]
-	~~[[Category: Khersonsky, O~~]]	+

OCA: Protected "6fhf" [edit=sysop:move=sysop]

OCA — Wed, 24 Jan 2018 17:26:38 GMT

Protected "6fhf" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:26, 24 January 2018

OCA: New page: '''Unreleased structure''' The entry 6fhf is ON HOLD Authors: Lapidot, G., Khersonsky, O., Lipsh, R., Dym, O., Albeck, S., Rogotner, S., Fleishman, J.S. Description: Highly active enzy...

OCA — Wed, 24 Jan 2018 17:26:36 GMT

New page: '''Unreleased structure''' The entry 6fhf is ON HOLD Authors: Lapidot, G., Khersonsky, O., Lipsh, R., Dym, O., Albeck, S., Rogotner, S., Fleishman, J.S. Description: Highly active enzy...

New page

'''Unreleased structure'''

The entry 6fhf is ON HOLD

Authors: Lapidot, G., Khersonsky, O., Lipsh, R., Dym, O., Albeck, S., Rogotner, S., Fleishman, J.S.

Description: Highly active enzymes by automated modular backbone assembly and sequence design
[[Category: Unreleased Structures]]
[[Category: Lapidot, G]]
[[Category: Albeck, S]]
[[Category: Lipsh, R]]
[[Category: Rogotner, S]]
[[Category: Fleishman, J.S]]
[[Category: Dym, O]]
[[Category: Khersonsky, O]]