6fhy - Revision history

OCA at 09:28, 30 October 2019

OCA — Wed, 30 Oct 2019 09:28:18 GMT

←Older revision		Revision as of 09:28, 30 October 2019
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	==Photorhabdus asymbiotica lectin (PHL) in complex with synthetic C-fucoside==		==Photorhabdus asymbiotica lectin (PHL) in complex with synthetic C-fucoside==
-	<StructureSection load='6fhy' size='340' side='right' caption='[[6fhy]], [[Resolution\|resolution]] 1.86Å' scene=''>	+	<StructureSection load='6fhy' size='340' side='right'caption='[[6fhy]], [[Resolution\|resolution]] 1.86Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6fhy]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FHY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6fhy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43950 Atcc 43950]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FHY FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DS8:(2~{S},3~{S},4~{R},5~{S},6~{S})-2-[[(2~{S},4~{R},6~{R})-2-(hydroxymethyl)-6-oxidanyl-oxan-4-yl]methyl]-6-methyl-oxane-3,4,5-triol'>DS8</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DS8:(2~{S},3~{S},4~{R},5~{S},6~{S})-2-[[(2~{S},4~{R},6~{R})-2-(hydroxymethyl)-6-oxidanyl-oxan-4-yl]methyl]-6-methyl-oxane-3,4,5-triol'>DS8</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PAU_00698 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=291112 ATCC 43950])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fhy OCA], [http://pdbe.org/6fhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fhy RCSB], [http://www.ebi.ac.uk/pdbsum/6fhy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fhy ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fhy OCA], [http://pdbe.org/6fhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fhy RCSB], [http://www.ebi.ac.uk/pdbsum/6fhy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fhy ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A recently described bangle lectin (PHL) from the bacterium Photorhabdus asymbiotica was identified as a mainly fucose-binding protein that could play an important role in the host-pathogen interaction and in the modulation of host immune response. Structural studies showed that PHL is a homo-dimer that contains up to seven L-fucose-specific binding sites per monomer. For these reasons, potential ligands of the PHL lectin: alpha-L-fucopyranosyl-containing mono-, di-, tetra-, hexa- and dodecavalent ligands were tested. Two types of polyvalent structures were investigated - calix[4]arenes and dendrimers. The shared feature of all these structures was a C-glycosidic bond instead of the more common but physiologically unstable O-glycosidic bond. The inhibition potential of the tested structures was assessed using different techniques - hemagglutination, surface plasmon resonance, isothermal titration calorimetry, and cell cross-linking. All the ligands proved to be better than free L-fucose. The most active hexavalent dendrimer exhibited affinity three orders of magnitude higher than that of standard L-fucose. To determine the binding mode of some ligands, crystal complex PHL/fucosides 2 - 4 were prepared and studied using X-ray crystallography. The electron density in complexes proved the presence of the compounds in 6 out of 7 fucose-binding sites.
		+
		+	Fucosylated inhibitors of recently identified bangle lectin from Photorhabdus asymbiotica.,Paulikova G, Houser J, Kasakova M, Oroszova B, Bertolotti B, Parkan K, Moravcova J, Wimmerova M Sci Rep. 2019 Oct 17;9(1):14904. doi: 10.1038/s41598-019-51357-9. PMID:31624296<ref>PMID:31624296</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6fhy" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 43950]]
		+	[[Category: Large Structures]]
	[[Category: Houser, J]]		[[Category: Houser, J]]
	[[Category: Jancarikova, G]]		[[Category: Jancarikova, G]]

OCA at 08:22, 30 January 2019

OCA — Wed, 30 Jan 2019 08:22:31 GMT

←Older revision		Revision as of 08:22, 30 January 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6fhy is ~~ON HOLD~~	+	==Photorhabdus asymbiotica lectin (PHL) in complex with synthetic C-fucoside==
-		+	<StructureSection load='6fhy' size='340' side='right' caption='[[6fhy]], [[Resolution\|resolution]] 1.86Å' scene=''>
-	~~Authors~~: ~~Houser~~, J., ~~Jancarikova~~, G., ~~Wimmerova~~, M.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6fhy]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FHY FirstGlance]. <br>
-	~~Description~~: ~~Photorhabdus asymbiotica lectin (PHL) in complex with synthetic C-fucoside~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DS8:(2~{S},3~{S},4~{R},5~{S},6~{S})-2-[[(2~{S},4~{R},6~{R})-2-(hydroxymethyl)-6-oxidanyl-oxan-4-yl]methyl]-6-methyl-oxane-3,4,5-triol'>DS8</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fhy OCA], [http://pdbe.org/6fhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fhy RCSB], [http://www.ebi.ac.uk/pdbsum/6fhy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fhy ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Houser, J]]
		+	[[Category: Jancarikova, G]]
	[[Category: Wimmerova, M]]		[[Category: Wimmerova, M]]
-	[[Category: ~~Jancarikova, G~~]]	+	[[Category: C-fucoside]]
-	[[Category: ~~Houser, J~~]]	+	[[Category: Lectin]]
		+	[[Category: Seven-bladed beta-propeller]]
		+	[[Category: Sugar binding protein]]

OCA at 05:15, 7 February 2018

OCA — Wed, 07 Feb 2018 05:15:45 GMT

←Older revision		Revision as of 05:15, 7 February 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6fhy is ON HOLD ~~until sometime in the future~~	+	The entry 6fhy is ON HOLD

-	Authors: Houser, J., Jancarikova, G~~., Kasakova, M~~., Wimmerova, M~~., Moravcova, J~~.	+	Authors: Houser, J., Jancarikova, G., Wimmerova, M.

	Description: Photorhabdus asymbiotica lectin (PHL) in complex with synthetic C-fucoside		Description: Photorhabdus asymbiotica lectin (PHL) in complex with synthetic C-fucoside
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	[[Category: Wimmerova, M]]		[[Category: Wimmerova, M]]
	[[Category: Jancarikova, G]]		[[Category: Jancarikova, G]]
-	[[Category: Moravcova, J]]
	[[Category: Houser, J]]		[[Category: Houser, J]]
-	[[Category: Kasakova, M]]

OCA: Protected "6fhy" [edit=sysop:move=sysop]

OCA — Wed, 24 Jan 2018 17:28:15 GMT

Protected "6fhy" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:28, 24 January 2018

OCA: New page: '''Unreleased structure''' The entry 6fhy is ON HOLD until sometime in the future Authors: Houser, J., Jancarikova, G., Kasakova, M., Wimmerova, M., Moravcova, J. Description: Photorha...

OCA — Wed, 24 Jan 2018 17:28:14 GMT

New page: '''Unreleased structure''' The entry 6fhy is ON HOLD until sometime in the future Authors: Houser, J., Jancarikova, G., Kasakova, M., Wimmerova, M., Moravcova, J. Description: Photorha...

New page

'''Unreleased structure'''

The entry 6fhy is ON HOLD until sometime in the future

Authors: Houser, J., Jancarikova, G., Kasakova, M., Wimmerova, M., Moravcova, J.

Description: Photorhabdus asymbiotica lectin (PHL) in complex with synthetic C-fucoside
[[Category: Unreleased Structures]]
[[Category: Wimmerova, M]]
[[Category: Jancarikova, G]]
[[Category: Moravcova, J]]
[[Category: Houser, J]]
[[Category: Kasakova, M]]