OCA at 07:44, 21 March 2018

OCA — Wed, 21 Mar 2018 07:44:56 GMT

←Older revision		Revision as of 07:44, 21 March 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6fkw is ~~ON HOLD~~	+	==Europium-containing methanol dehydrogenase==
		+	<StructureSection load='6fkw' size='340' side='right' caption='[[6fkw]], [[Resolution\|resolution]] 1.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6fkw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Methylacidiphilum_fumariolicum_solv Methylacidiphilum fumariolicum solv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FKW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FKW FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EU:EUROPIUM+ION'>EU</scene>, <scene name='pdbligand=PQQ:PYRROLOQUINOLINE+QUINONE'>PQQ</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fkw OCA], [http://pdbe.org/6fkw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fkw RCSB], [http://www.ebi.ac.uk/pdbsum/6fkw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fkw ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Since the discovery of the biological relevance of rare earth elements (REE) for numerous different bacteria, the questions of the advantage of REE in the active site of methanol dehydrogenase (MDH) over calcium(II) and why bacteria prefer light REE have been a subject of debate. Here we report the cultivation and purification of the strictly REE-dependent methanotrophic bacterium Methyl-acidiphilum fumariolicum SolV with europium(III) as well as structural and kinetic analyses of the first Eu-substituted methanol dehydrogenase. Crystal structure determination of the Eu-MDH demonstrated that overall no major structural changes were induced by converting to this REE. Circular Dichroism (CD) measurements were used to determine optimal conditions for kinetic assays and inductively-coupled plasma mass-spectrometry (ICP-MS) showed 70% incorporation of Eu in the enzyme. Our studies explain why bacterial growth of SolV with Eu3+ is significantly slower than with La3+/Ce3+/Pr3+: Eu-MDH possesses a decreased catalytic efficiency and affinity for the substrate. Although rare earth elements have similar properties, the differences in ionic radii and coordination numbers across the series significantly impact MDH efficiency.

-	~~Authors~~: ~~Barends~~, T., Dietl, A.	+	Similar but not the same: First Kinetic and Structural Analyses of a Methanol Dehydrogenase Containing a Europium Ion in the Active Site.,Jahn B, Pol A, Lumpe H, Barends T, Dietl A, Hogendoorn C, Op den Camp H, Daumann L Chembiochem. 2018 Mar 10. doi: 10.1002/cbic.201800130. PMID:29524328<ref>PMID:29524328</ref>

-	~~Description: Europium-containing methanol dehydrogenase~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Dietl, A~~]]	+	<div class="pdbe-citations 6fkw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Methylacidiphilum fumariolicum solv]]
	[[Category: Barends, T]]		[[Category: Barends, T]]
		+	[[Category: Dietl, A]]
		+	[[Category: Methanol dehydrogenase]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Pqq]]
		+	[[Category: Rare earth element]]

OCA: Protected "6fkw" [edit=sysop:move=sysop]

OCA — Wed, 31 Jan 2018 04:59:04 GMT

Protected "6fkw" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:59, 31 January 2018

OCA: New page: '''Unreleased structure''' The entry 6fkw is ON HOLD Authors: Barends, T., Dietl, A. Description: Europium-containing methanol dehydrogenase Category: Unreleased Structures [[Categ...

OCA — Wed, 31 Jan 2018 04:59:03 GMT

New page: '''Unreleased structure''' The entry 6fkw is ON HOLD Authors: Barends, T., Dietl, A. Description: Europium-containing methanol dehydrogenase Category: Unreleased Structures [[Categ...

New page

'''Unreleased structure'''

The entry 6fkw is ON HOLD

Authors: Barends, T., Dietl, A.

Description: Europium-containing methanol dehydrogenase
[[Category: Unreleased Structures]]
[[Category: Dietl, A]]
[[Category: Barends, T]]

6fkw - Revision history

OCA at 07:44, 21 March 2018

OCA: Protected "6fkw" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6fkw is ON HOLD Authors: Barends, T., Dietl, A. Description: Europium-containing methanol dehydrogenase Category: Unreleased Structures [[Categ...