6fli - Revision history

OCA at 12:29, 9 May 2024

OCA — Thu, 09 May 2024 12:29:29 GMT

←Older revision		Revision as of 12:29, 9 May 2024
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	<StructureSection load='6fli' size='340' side='right'caption='[[6fli]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='6fli' size='340' side='right'caption='[[6fli]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6fli]] is a 5 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Endosymbiont_of_tevnia_jerichonana_~~(~~vent_tica~~) Endosymbiont of ~~tevnia~~ jerichonana (vent ~~tica~~)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FLI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6FLI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6fli]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Endosymbiont_of_Tevnia_jerichonana_(vent_Tica) Endosymbiont of Tevnia jerichonana (vent Tica)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FLI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6FLI FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=BNG:B~~-~~NONYLGLUCOSIDE'>BNG</scene>~~, ~~<scene name='pdbligand=DQZ:~~3~~-(4-bromophenyl)propanoic+acid'>DQZ</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[6fl9\|6fl9]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=DQZ:3-(4-bromophenyl)propanoic+acid'>DQZ</scene></td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~"><~~b>[[Gene\|Gene~~:~~]]</b~~></td><~~td class~~=~~"sblockDat">TevJSym_bc00020 ([http~~:~~//www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1049564 endosymbiont of Tevnia jerichonana~~ (~~vent Tica)]~~)</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6fli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fli OCA], [https://pdbe.org/6fli PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6fli RCSB], [https://www.ebi.ac.uk/pdbsum/6fli PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6fli ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6fli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fli OCA], [~~http~~://pdbe.org/6fli PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6fli RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6fli PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6fli ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G2FID1_9GAMM G2FID1_9GAMM]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6fli" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6fli" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ion channels 3D structures\|Ion channels 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Delarue, M]]	+	[[Category: Delarue M]]
-	[[Category: Hu, H]]	+	[[Category: Hu H]]
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Pentameric ligand-gated ion channel~~]]	+

OCA at 06:10, 12 June 2019

OCA — Wed, 12 Jun 2019 06:10:51 GMT

←Older revision		Revision as of 06:10, 12 June 2019
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	==The active form of a pentameric ion channel (sTeLIC) gated by alkaline pH - Co-crystallization with 4-bromo cinnamic acid==		==The active form of a pentameric ion channel (sTeLIC) gated by alkaline pH - Co-crystallization with 4-bromo cinnamic acid==
-	<StructureSection load='6fli' size='340' side='right' caption='[[6fli]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='6fli' size='340' side='right'caption='[[6fli]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6fli]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FLI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FLI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6fli]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Endosymbiont_of_tevnia_jerichonana_(vent_tica) Endosymbiont of tevnia jerichonana (vent tica)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FLI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FLI FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=DQZ:3-(4-bromophenyl)propanoic+acid'>DQZ</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=DQZ:3-(4-bromophenyl)propanoic+acid'>DQZ</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6fl9\|6fl9]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6fl9\|6fl9]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TevJSym_bc00020 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1049564 endosymbiont of Tevnia jerichonana (vent Tica)])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fli OCA], [http://pdbe.org/6fli PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fli RCSB], [http://www.ebi.ac.uk/pdbsum/6fli PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fli ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fli OCA], [http://pdbe.org/6fli PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fli RCSB], [http://www.ebi.ac.uk/pdbsum/6fli PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fli ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Delarue, M]]		[[Category: Delarue, M]]
-	[[Category: HU, H]]	+	[[Category: Hu, H]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]
	[[Category: Pentameric ligand-gated ion channel]]		[[Category: Pentameric ligand-gated ion channel]]

OCA at 05:36, 16 May 2018

OCA — Wed, 16 May 2018 05:36:35 GMT

←Older revision		Revision as of 05:36, 16 May 2018
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-	'''Unreleased structure'''

-	The ~~entry~~ 6fli is ~~ON HOLD~~	+	==The active form of a pentameric ion channel (sTeLIC) gated by alkaline pH - Co-crystallization with 4-bromo cinnamic acid==
		+	<StructureSection load='6fli' size='340' side='right' caption='[[6fli]], [[Resolution\|resolution]] 3.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6fli]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FLI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FLI FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=DQZ:3-(4-bromophenyl)propanoic+acid'>DQZ</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6fl9\|6fl9]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fli OCA], [http://pdbe.org/6fli PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fli RCSB], [http://www.ebi.ac.uk/pdbsum/6fli PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fli ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Pentameric ligand-gated ion channels (pLGICs) constitute a widespread class of ion channels, present in archaea, bacteria, and eukaryotes. Upon binding of their agonists in the extracellular domain, the transmembrane pore opens, allowing ions to go through, via a gating mechanism that can be modulated by a number of drugs. Even though high-resolution structural information on pLGICs has increased in a spectacular way in recent years, both in bacterial and in eukaryotic systems, the structure of the open channel conformation of some intensively studied receptors whose structures are known in a nonactive (closed) form, such as Erwinia chrysanthemi pLGIC (ELIC), is still lacking. Here we describe a gammaproteobacterial pLGIC from an endo-symbiont of Tevnia jerichonana (sTeLIC), whose sequence is closely related to the pLGIC from ELIC with 28% identity. We provide an X-ray crystallographic structure at 2.3 A in an active conformation, where the pore is found to be more open than any current conformation found for pLGICs. In addition, two charged restriction rings are present in the vestibule. Functional characterization shows sTeLIC to be a cationic channel activated at alkaline pH. It is inhibited by divalent cations, but not by quaternary ammonium ions, such as tetramethylammonium. Additionally, we found that sTeLIC is allosterically potentiated by aromatic amino acids Phe and Trp, as well as their derivatives, such as 4-bromo-cinnamate, whose cocrystal structure reveals a vestibular binding site equivalent to, but more deeply buried than, the one already described for benzodiazepines in ELIC.

-	~~Authors: HU~~, H., ~~Delarue~~, M.	+	Crystal structures of a pentameric ion channel gated by alkaline pH show a widely open pore and identify a cavity for modulation.,Hu H, Nemecz A, Van Renterghem C, Fourati Z, Sauguet L, Corringer PJ, Delarue M Proc Natl Acad Sci U S A. 2018 Apr 24;115(17):E3959-E3968. doi:, 10.1073/pnas.1717700115. Epub 2018 Apr 9. PMID:29632192<ref>PMID:29632192</ref>

-	~~Description: The active form of~~ a ~~pentameric ion channel (sTeLIC) gated by alkaline pH~~ -Co-~~crystallization with 4-bromo cinnamic acid~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 6fli" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Delarue, M]]		[[Category: Delarue, M]]
-	[[Category: Hu, H]]	+	[[Category: HU, H]]
		+	[[Category: Membrane protein]]
		+	[[Category: Pentameric ligand-gated ion channel]]

OCA: Protected "6fli" [edit=sysop:move=sysop]

OCA — Wed, 31 Jan 2018 05:00:22 GMT

Protected "6fli" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:00, 31 January 2018

OCA: New page: '''Unreleased structure''' The entry 6fli is ON HOLD Authors: HU, H., Delarue, M. Description: The active form of a pentameric ion channel (sTeLIC) gated by alkaline pH -Co-crystalliza...

OCA — Wed, 31 Jan 2018 05:00:21 GMT

New page: '''Unreleased structure''' The entry 6fli is ON HOLD Authors: HU, H., Delarue, M. Description: The active form of a pentameric ion channel (sTeLIC) gated by alkaline pH -Co-crystalliza...

New page

'''Unreleased structure'''

The entry 6fli is ON HOLD

Authors: HU, H., Delarue, M.

Description: The active form of a pentameric ion channel (sTeLIC) gated by alkaline pH -Co-crystallization with 4-bromo cinnamic acid
[[Category: Unreleased Structures]]
[[Category: Delarue, M]]
[[Category: Hu, H]]