6fop - Revision history

OCA at 06:03, 10 October 2019

OCA — Thu, 10 Oct 2019 06:03:33 GMT

←Older revision		Revision as of 06:03, 10 October 2019
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	<StructureSection load='6fop' size='340' side='right'caption='[[6fop]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='6fop' size='340' side='right'caption='[[6fop]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6fop]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FOP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FOP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6fop]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FOP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FOP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cthe_0660 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=203119 Clostridium thermocellum])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fop OCA], [http://pdbe.org/6fop PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fop RCSB], [http://www.ebi.ac.uk/pdbsum/6fop PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fop ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fop OCA], [http://pdbe.org/6fop PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fop RCSB], [http://www.ebi.ac.uk/pdbsum/6fop PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fop ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The family 81 glycoside hydrolase (GH81) from Clostridium thermocellum is a beta-1,3-glucanase belonging to cellulosomal complex. The gene encoding GH81 from Clostridium thermocellum (CtLam81A) was cloned and expressed displaying a molecular mass of ~82kDa. CtLam81A showed maximum activity against laminarin (100U/mg), followed by curdlan (65U/mg), at pH7.0 and 75 degrees C. CtLam81A displayed Km, 2.1+/-0.12mg/ml and Vmax, 109+/-1.8U/mg, against laminarin under optimized conditions. CtLam81A activity was significantly enhanced by Ca(2+) or Mg(2+) ions. Melting curve analysis of CtLam81A showed an increase in melting temperature from 91 degrees C to 96 degrees C by Ca(2+) or Mg(2+) ions and decreased to 82 degrees C by EDTA, indicating that Ca(2+) and Mg(2+) ions may be involved in catalysis and in maintaining structural integrity. TLC and MALDI-TOF analysis of beta-1,3-glucan hydrolysed products released initially, showed beta-1,3-glucan-oligosaccharides degree of polymerization (DP) from DP2 to DP7, confirming an endo-mode of action. The catalytically inactive mutant CtLam81A-E515A generated by site-directed mutagenesis was co-crystallized and tetragonal crystals diffracting up to 1.4A resolution were obtained. CtLam81A-E515A contained 15 alpha-helices and 38 beta-strands forming a four-domain structure viz. a beta-sandwich domain I at N-terminal, an alpha/beta-domain II, an (alpha/alpha)6 barrel domain III, and a small 5-stranded beta-sandwich domain IV.
		+
		+	Novel insights into the degradation of beta-1,3-glucans by the cellulosome of Clostridium thermocellum revealed by structure and function studies of a family 81 glycoside hydrolase.,Kumar K, Correia MAS, Pires VMR, Dhillon A, Sharma K, Rajulapati V, Fontes CMGA, Carvalho AL, Goyal A Int J Biol Macromol. 2018 Oct 1;117:890-901. doi: 10.1016/j.ijbiomac.2018.06.003., Epub 2018 Jun 2. PMID:29870811<ref>PMID:29870811</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6fop" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Clostridium thermocellum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Carvalho, A L]]		[[Category: Carvalho, A L]]
	[[Category: Correia, M A.S C]]		[[Category: Correia, M A.S C]]
-	[[Category: Clostridium thermocellum]]
	[[Category: Gh81]]		[[Category: Gh81]]
	[[Category: Glucanase]]		[[Category: Glucanase]]

OCA at 11:59, 13 March 2019

OCA — Wed, 13 Mar 2019 11:59:10 GMT

←Older revision		Revision as of 11:59, 13 March 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6fop is ~~ON HOLD~~	+	==Glycoside hydrolase family 81 from Clostridium thermocellum (CtLam81A), Mutant E515A==
-		+	<StructureSection load='6fop' size='340' side='right'caption='[[6fop]], [[Resolution\|resolution]] 1.40Å' scene=''>
-	~~Authors~~: ~~Correia~~, M.A.S.C., ~~Carvalho~~, A.L.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6fop]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FOP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FOP FirstGlance]. <br>
-	~~Description: Glycoside hydrolase family 81 from Clostridium thermocellum (CtLam81A), Mutant E515A~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fop OCA], [http://pdbe.org/6fop PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fop RCSB], [http://www.ebi.ac.uk/pdbsum/6fop PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fop ProSAT]</span></td></tr>
-	[[Category: Correia, M.A.S.C]]	+	</table>
-	[[Category: ~~Carvalho, A.L~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Carvalho, A L]]
		+	[[Category: Correia, M A.S C]]
		+	[[Category: Clostridium thermocellum]]
		+	[[Category: Gh81]]
		+	[[Category: Glucanase]]
		+	[[Category: Glycoside hydrolase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Laminarin]]

OCA at 05:48, 14 June 2018

OCA — Thu, 14 Jun 2018 05:48:58 GMT

←Older revision		Revision as of 05:48, 14 June 2018
Line 3:		Line 3:
	The entry 6fop is ON HOLD		The entry 6fop is ON HOLD

-	Authors: Correia, M.A.S., Carvalho, A.L.	+	Authors: Correia, M.A.S.C., Carvalho, A.L.

	Description: Glycoside hydrolase family 81 from Clostridium thermocellum (CtLam81A), Mutant E515A		Description: Glycoside hydrolase family 81 from Clostridium thermocellum (CtLam81A), Mutant E515A
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Correia, M.A.S]]	+	[[Category: Correia, M.A.S.C]]
	[[Category: Carvalho, A.L]]		[[Category: Carvalho, A.L]]

OCA: Protected "6fop" [edit=sysop:move=sysop]

OCA — Thu, 15 Feb 2018 05:30:45 GMT

Protected "6fop" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:30, 15 February 2018

OCA: New page: '''Unreleased structure''' The entry 6fop is ON HOLD Authors: Correia, M.A.S., Carvalho, A.L. Description: Glycoside hydrolase family 81 from Clostridium thermocellum (CtLam81A), Mutan...

OCA — Thu, 15 Feb 2018 05:30:44 GMT

New page: '''Unreleased structure''' The entry 6fop is ON HOLD Authors: Correia, M.A.S., Carvalho, A.L. Description: Glycoside hydrolase family 81 from Clostridium thermocellum (CtLam81A), Mutan...

New page

'''Unreleased structure'''

The entry 6fop is ON HOLD

Authors: Correia, M.A.S., Carvalho, A.L.

Description: Glycoside hydrolase family 81 from Clostridium thermocellum (CtLam81A), Mutant E515A
[[Category: Unreleased Structures]]
[[Category: Correia, M.A.S]]
[[Category: Carvalho, A.L]]