6g09 - Revision history

OCA at 08:39, 17 October 2018

OCA — Wed, 17 Oct 2018 08:39:52 GMT

←Older revision		Revision as of 08:39, 17 October 2018
Line 3:		Line 3:
	<StructureSection load='6g09' size='340' side='right' caption='[[6g09]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='6g09' size='340' side='right' caption='[[6g09]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6g09]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G09 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G09 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6g09]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Tertt Tertt]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G09 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G09 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BXP:4-O-BETA-D-XYLOPYRANOSYL-BETA-D-XYLOPYRANOSE'>BXP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BXP:4-O-BETA-D-XYLOPYRANOSYL-BETA-D-XYLOPYRANOSE'>BXP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6g00\|6g00]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6g00\|6g00]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TERTU_4506 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=377629 TERTT])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g09 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g09 OCA], [http://pdbe.org/6g09 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g09 RCSB], [http://www.ebi.ac.uk/pdbsum/6g09 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g09 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g09 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g09 OCA], [http://pdbe.org/6g09 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g09 RCSB], [http://www.ebi.ac.uk/pdbsum/6g09 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g09 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The biological conversion of lignocellulosic matter into high-value chemicals or biofuels is of increasing industrial importance as the sector slowly transitions away from nonrenewable sources. Many industrial processes involve the use of cellulolytic enzyme cocktails - a selection of glycoside hydrolases and, increasingly, polysaccharide oxygenases - to break down recalcitrant plant polysaccharides. ORFs from the genome of Teredinibacter turnerae, a symbiont hosted within the gills of marine shipworms, were identified in order to search for enzymes with desirable traits. Here, a putative T. turnerae glycoside hydrolase from family 8, hereafter referred to as TtGH8, is analysed. The enzyme is shown to be active against beta-1,4-xylan and mixed-linkage (beta-1,3,beta-1,4) marine xylan. Kinetic parameters, obtained using high-performance anion-exchange chromatography with pulsed amperometric detection and 3,5-dinitrosalicyclic acid reducing-sugar assays, show that TtGH8 catalyses the hydrolysis of beta-1,4-xylohexaose with a kcat/Km of 7.5 x 10(7) M(-1) min(-1) but displays maximal activity against mixed-linkage polymeric xylans, hinting at a primary role in the degradation of marine polysaccharides. The three-dimensional structure of TtGH8 was solved in uncomplexed and xylobiose-, xylotriose- and xylohexaose-bound forms at approximately 1.5 A resolution; the latter was consistent with the greater kcat/Km for hexasaccharide substrates. A (2,5)B boat conformation observed in the -1 position of bound xylotriose is consistent with the proposed conformational itinerary for this class of enzyme. This work shows TtGH8 to be effective at the degradation of xylan-based substrates, notably marine xylan, further exemplifying the potential of T. turnerae for effective and diverse biomass degradation.
		+
		+	Structure and function of a glycoside hydrolase family 8 endoxylanase from Teredinibacter turnerae.,Fowler CA, Hemsworth GR, Cuskin F, Hart S, Turkenburg J, Gilbert HJ, Walton PH, Davies GJ Acta Crystallogr D Struct Biol. 2018 Oct 1;74(Pt 10):946-955. doi:, 10.1107/S2059798318009737. Epub 2018 Oct 2. PMID:30289404<ref>PMID:30289404</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6g09" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Tertt]]
	[[Category: Davies, G J]]		[[Category: Davies, G J]]
	[[Category: Fowler, C A]]		[[Category: Fowler, C A]]

OCA at 08:12, 10 October 2018

OCA — Wed, 10 Oct 2018 08:12:30 GMT

←Older revision		Revision as of 08:12, 10 October 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6g09 is ~~ON HOLD~~	+	==Crystal Structure of a GH8 xylobiose complex from Teredinibacter turnerae==
-		+	<StructureSection load='6g09' size='340' side='right' caption='[[6g09]], [[Resolution\|resolution]] 1.40Å' scene=''>
-	~~Authors~~: ~~Fowler~~, C.A., ~~Davies~~, G.J., ~~Walton~~, P.H.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6g09]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G09 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G09 FirstGlance]. <br>
-	~~Description: Crystal Structure of a GH8 xylobiose complex from Teredinibacter turnerae~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BXP:4-O-BETA-D-XYLOPYRANOSYL-BETA-D-XYLOPYRANOSE'>BXP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6g00\|6g00]]</td></tr>
-	[[Category: Fowler, C.A]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g09 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g09 OCA], [http://pdbe.org/6g09 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g09 RCSB], [http://www.ebi.ac.uk/pdbsum/6g09 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g09 ProSAT]</span></td></tr>
-	[[Category: ~~Davies~~, ~~G.J~~]]	+	</table>
-	[[Category: ~~Walton, P.H~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Davies, G J]]
		+	[[Category: Fowler, C A]]
		+	[[Category: Walton, P H]]
		+	[[Category: Carbohydrate]]
		+	[[Category: Hydrolase]]
		+	[[Category: Native]]
		+	[[Category: Xylanse]]

OCA: Protected "6g09" [edit=sysop:move=sysop]

OCA — Wed, 28 Mar 2018 05:26:28 GMT

Protected "6g09" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:26, 28 March 2018

OCA: New page: '''Unreleased structure''' The entry 6g09 is ON HOLD Authors: Fowler, C.A., Davies, G.J., Walton, P.H. Description: Crystal Structure of a GH8 xylobiose complex from Teredinibacter tur...

OCA — Wed, 28 Mar 2018 05:26:27 GMT

New page: '''Unreleased structure''' The entry 6g09 is ON HOLD Authors: Fowler, C.A., Davies, G.J., Walton, P.H. Description: Crystal Structure of a GH8 xylobiose complex from Teredinibacter tur...

New page

'''Unreleased structure'''

The entry 6g09 is ON HOLD

Authors: Fowler, C.A., Davies, G.J., Walton, P.H.

Description: Crystal Structure of a GH8 xylobiose complex from Teredinibacter turnerae
[[Category: Unreleased Structures]]
[[Category: Fowler, C.A]]
[[Category: Davies, G.J]]
[[Category: Walton, P.H]]