6g26 - Revision history

OCA at 07:49, 6 November 2019

OCA — Wed, 06 Nov 2019 07:49:38 GMT

←Older revision		Revision as of 07:49, 6 November 2019
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	==The crystal structure of the Burkholderia pseudomallei HicAB complex==		==The crystal structure of the Burkholderia pseudomallei HicAB complex==
-	<StructureSection load='6g26' size='340' side='right' caption='[[6g26]], [[Resolution\|resolution]] 2.49Å' scene=''>	+	<StructureSection load='6g26' size='340' side='right'caption='[[6g26]], [[Resolution\|resolution]] 2.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6g26]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Burps Burps]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G26 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G26 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6g26]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Burps Burps]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G26 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G26 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[gc1n\|gc1n]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BPSS0391 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272560 BURPS]), BPSS0390 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272560 BURPS])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BPSS0391 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272560 BURPS]), BPSS0390 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272560 BURPS])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g26 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g26 OCA], [http://pdbe.org/6g26 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g26 RCSB], [http://www.ebi.ac.uk/pdbsum/6g26 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g26 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g26 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g26 OCA], [http://pdbe.org/6g26 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g26 RCSB], [http://www.ebi.ac.uk/pdbsum/6g26 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g26 ProSAT]</span></td></tr>
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	</StructureSection>		</StructureSection>
	[[Category: Burps]]		[[Category: Burps]]
		+	[[Category: Large Structures]]
	[[Category: Crump, M P]]		[[Category: Crump, M P]]
	[[Category: Isupov, M N]]		[[Category: Isupov, M N]]

OCA at 08:41, 26 December 2018

OCA — Wed, 26 Dec 2018 08:41:35 GMT

←Older revision		Revision as of 08:41, 26 December 2018
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	<StructureSection load='6g26' size='340' side='right' caption='[[6g26]], [[Resolution\|resolution]] 2.49Å' scene=''>		<StructureSection load='6g26' size='340' side='right' caption='[[6g26]], [[Resolution\|resolution]] 2.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6g26]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G26 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G26 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6g26]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Burps Burps]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G26 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G26 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[gc1n\|gc1n]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[gc1n\|gc1n]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BPSS0391 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272560 BURPS]), BPSS0390 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272560 BURPS])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g26 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g26 OCA], [http://pdbe.org/6g26 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g26 RCSB], [http://www.ebi.ac.uk/pdbsum/6g26 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g26 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g26 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g26 OCA], [http://pdbe.org/6g26 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g26 RCSB], [http://www.ebi.ac.uk/pdbsum/6g26 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g26 ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Burps]]
	[[Category: Crump, M P]]		[[Category: Crump, M P]]
	[[Category: Isupov, M N]]		[[Category: Isupov, M N]]

OCA at 06:56, 31 October 2018

OCA — Wed, 31 Oct 2018 06:56:16 GMT

←Older revision		Revision as of 06:56, 31 October 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The ~~entry~~ 6g26 is ~~ON HOLD until Paper~~ Publication	+	==The crystal structure of the Burkholderia pseudomallei HicAB complex==
		+	<StructureSection load='6g26' size='340' side='right' caption='[[6g26]], [[Resolution\|resolution]] 2.49Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6g26]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G26 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G26 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[gc1n\|gc1n]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g26 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g26 OCA], [http://pdbe.org/6g26 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g26 RCSB], [http://www.ebi.ac.uk/pdbsum/6g26 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g26 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Toxin-antitoxin (TA) systems are present in many bacteria and play important roles in bacterial growth, physiology, and pathogenicity. Those that are best studied are the type II TA systems, in which both toxins and antitoxins are proteins. The HicAB system is one of the prototypic TA systems, found in many bacterial species. Complex interactions between the protein toxin (HicA), the protein antitoxin (HicB), and the DNA upstream of the encoding genes regulate the activity of this system, but few structural details are available about how HicA destabilizes the HicB-DNA complex. Here, we determined the X-ray structures of HicB and the HicAB complex to 1.8 and 2.5 A resolution respectively and characterized their DNA interactions. This revealed that HicB forms a tetramer and HicA and HicB form a hetero-octameric complex that involves structural reorganization of the C-terminal (DNA-binding) region of HicB. Our observations indicated that HicA has a profound impact on binding of HicB to DNA sequences upstream of hicAB in a stoichiometric-dependent way. At low ratios of HicA:HicB, there was no effect on DNA binding, but at higher ratios, the affinity for DNA declined cooperatively, driving dissociation of the HicA:HicB:DNA complex.These results reveal the structural mechanisms by which HicA de-represses the HicB-DNA complex.

-	~~Authors~~:	+	The molecular basis of protein toxin HicA-dependent binding of the protein antitoxin HicB to DNA.,Winter AJ, Williams C, Isupov MN, Crocker H, Gromova M, Marsh P, Wilkinson OJ, Dillingham MS, Harmer NJ, Titball RW, Crump MP J Biol Chem. 2018 Oct 18. pii: RA118.005173. doi: 10.1074/jbc.RA118.005173. PMID:30337369<ref>PMID:30337369</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6g26" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Crump, M P]]
		+	[[Category: Isupov, M N]]
		+	[[Category: Williams, C]]
		+	[[Category: Winter, A J]]
		+	[[Category: Antitoxin]]
		+	[[Category: N-terminal domain of the antitoxin hicb which acts as an inhibitor to hica]]

OCA at 04:47, 4 April 2018

OCA — Wed, 04 Apr 2018 04:47:38 GMT

←Older revision		Revision as of 04:47, 4 April 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6g26 is ON HOLD	+	The entry 6g26 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6g26" [edit=sysop:move=sysop]

OCA — Wed, 28 Mar 2018 05:31:36 GMT

Protected "6g26" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:31, 28 March 2018

OCA: New page: '''Unreleased structure''' The entry 6g26 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 28 Mar 2018 05:31:35 GMT

New page: '''Unreleased structure''' The entry 6g26 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6g26 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]