6g2f - Revision history

OCA at 12:33, 9 May 2024

OCA — Thu, 09 May 2024 12:33:12 GMT

←Older revision		Revision as of 12:33, 9 May 2024
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	<StructureSection load='6g2f' size='340' side='right'caption='[[6g2f]], [[Resolution\|resolution]] 1.74Å' scene=''>		<StructureSection load='6g2f' size='340' side='right'caption='[[6g2f]], [[Resolution\|resolution]] 1.74Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6g2f]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G2F OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6G2F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6g2f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G2F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6G2F FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=EHW:4~~-[~~5-(7-fluoranylquinolin-4-yl)-1-methyl-imidazol-4-yl~~]~~-3,5-dimethyl-~~1~~,2-oxazole'>EHW</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.74Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[6g24\|6g24]], [[6g25\|6g25]], [[6g27\|6g27]], [[6g29\|6g29]], [[6g2b\|6g2b]], [[6g2c\|6g2c]], [[6g2e\|6g2e]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EHW:4-[5-(7-fluoranylquinolin-4-yl)-1-methyl-imidazol-4-yl]-3,5-dimethyl-1,2-oxazole'>EHW</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[http~~:~~//en.wikipedia.org/wiki/Histone~~-~~lysine_N~~-~~methyltransferase Histone~~-~~lysine N~~-~~methyltransferase~~], ~~with EC number [http://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=2.~~1~~.1.43~~ 2~~.1.1.43]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6g2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g2f OCA], [https://pdbe.org/6g2f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6g2f RCSB], [https://www.ebi.ac.uk/pdbsum/6g2f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6g2f ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6g2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g2f OCA], [~~http~~://pdbe.org/6g2f PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6g2f RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6g2f PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6g2f ProSAT]</span></td></tr>	+
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/NSD3_HUMAN NSD3_HUMAN]] Note=Defects in WHSC1L1 may be involved in non small cell lung carcinomas (NSCLC). Amplified or overexpressed in NSCLC.<ref>PMID:15983384</ref> Note=A chromosomal aberration involving WHSC1L1 is found in childhood acute myeloid leukemia. Translocation t(8;11)(p11.2;p15) with NUP98.	+	[https://www.uniprot.org/uniprot/NSD3_HUMAN NSD3_HUMAN] Note=Defects in WHSC1L1 may be involved in non small cell lung carcinomas (NSCLC). Amplified or overexpressed in NSCLC.<ref>PMID:15983384</ref> Note=A chromosomal aberration involving WHSC1L1 is found in childhood acute myeloid leukemia. Translocation t(8;11)(p11.2;p15) with NUP98.
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/NSD3_HUMAN NSD3_HUMAN]] Histone methyltransferase. Preferentially methylates 'Lys-4' and 'Lys-27' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression.<ref>PMID:16682010</ref>	+	[https://www.uniprot.org/uniprot/NSD3_HUMAN NSD3_HUMAN] Histone methyltransferase. Preferentially methylates 'Lys-4' and 'Lys-27' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression.<ref>PMID:16682010</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Here, we report the fragment-based discovery of BI-9321, a potent, selective and cellular active antagonist of the NSD3-PWWP1 domain. The human NSD3 protein is encoded by the WHSC1L1 gene located in the 8p11-p12 amplicon, frequently amplified in breast and squamous lung cancer. Recently, it was demonstrated that the PWWP1 domain of NSD3 is required for the viability of acute myeloid leukemia cells. To further elucidate the relevance of NSD3 in cancer biology, we developed a chemical probe, BI-9321, targeting the methyl-lysine binding site of the PWWP1 domain with sub-micromolar in vitro activity and cellular target engagement at 1 microM. As a single agent, BI-9321 downregulates Myc messenger RNA expression and reduces proliferation in MOLM-13 cells. This first-in-class chemical probe BI-9321, together with the negative control BI-9466, will greatly facilitate the elucidation of the underexplored biological function of PWWP domains.
		+
		+	Fragment-based discovery of a chemical probe for the PWWP1 domain of NSD3.,Bottcher J, Dilworth D, Reiser U, Neumuller RA, Schleicher M, Petronczki M, Zeeb M, Mischerikow N, Allali-Hassani A, Szewczyk MM, Li F, Kennedy S, Vedadi M, Barsyte-Lovejoy D, Brown PJ, Huber KVM, Rogers CM, Wells CI, Fedorov O, Rumpel K, Zoephel A, Mayer M, Wunberg T, Bose D, Zahn S, Arnhof H, Berger H, Reiser C, Hormann A, Krammer T, Corcokovic M, Sharps B, Winkler S, Haring D, Cockcroft XL, Fuchs JE, Mullauer B, Weiss-Puxbaum A, Gerstberger T, Boehmelt G, Vakoc CR, Arrowsmith CH, Pearson M, McConnell DB Nat Chem Biol. 2019 Aug;15(8):822-829. doi: 10.1038/s41589-019-0310-x. Epub 2019 , Jul 8. PMID:31285596<ref>PMID:31285596</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6g2f" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Histone methyltransferase 3D structures\|Histone methyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Histone-lysine N-methyltransferase~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Boettcher, J]]	+	[[Category: Boettcher J]]
-	[[Category: Muellauer~~, B J~~]]	+	[[Category: Muellauer BJ]]
-	[[Category: Weiss-Puxbaum, A]]	+	[[Category: Weiss-Puxbaum A]]
-	[[Category: Zoephel, A]]	+	[[Category: Zoephel A]]
-	~~[[Category: Inhibitor]]~~	+
-	~~[[Category: Oncoprotein]]~~	+
-	~~[[Category: Pwwp domain~~]]	+

OCA at 06:45, 26 June 2019

OCA — Wed, 26 Jun 2019 06:45:44 GMT

←Older revision		Revision as of 06:45, 26 June 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6g2f is ~~ON HOLD until Paper Publication~~	+	==X-ray structure of NSD3-PWWP1 in complex with compound 16==
-		+	<StructureSection load='6g2f' size='340' side='right'caption='[[6g2f]], [[Resolution\|resolution]] 1.74Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6g2f]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G2F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G2F FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EHW:4-[5-(7-fluoranylquinolin-4-yl)-1-methyl-imidazol-4-yl]-3,5-dimethyl-1,2-oxazole'>EHW</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6g24\|6g24]], [[6g25\|6g25]], [[6g27\|6g27]], [[6g29\|6g29]], [[6g2b\|6g2b]], [[6g2c\|6g2c]], [[6g2e\|6g2e]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histone-lysine_N-methyltransferase Histone-lysine N-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.43 2.1.1.43] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g2f OCA], [http://pdbe.org/6g2f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g2f RCSB], [http://www.ebi.ac.uk/pdbsum/6g2f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g2f ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/NSD3_HUMAN NSD3_HUMAN]] Note=Defects in WHSC1L1 may be involved in non small cell lung carcinomas (NSCLC). Amplified or overexpressed in NSCLC.<ref>PMID:15983384</ref> Note=A chromosomal aberration involving WHSC1L1 is found in childhood acute myeloid leukemia. Translocation t(8;11)(p11.2;p15) with NUP98.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NSD3_HUMAN NSD3_HUMAN]] Histone methyltransferase. Preferentially methylates 'Lys-4' and 'Lys-27' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression.<ref>PMID:16682010</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Histone-lysine N-methyltransferase]]
		+	[[Category: Large Structures]]
		+	[[Category: Boettcher, J]]
		+	[[Category: Muellauer, B J]]
		+	[[Category: Weiss-Puxbaum, A]]
		+	[[Category: Zoephel, A]]
		+	[[Category: Inhibitor]]
		+	[[Category: Oncoprotein]]
		+	[[Category: Pwwp domain]]

OCA at 04:48, 4 April 2018

OCA — Wed, 04 Apr 2018 04:48:02 GMT

←Older revision		Revision as of 04:48, 4 April 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6g2f is ON HOLD	+	The entry 6g2f is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6g2f" [edit=sysop:move=sysop]

OCA — Wed, 28 Mar 2018 05:32:02 GMT

Protected "6g2f" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:32, 28 March 2018

OCA: New page: '''Unreleased structure''' The entry 6g2f is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 28 Mar 2018 05:32:01 GMT

New page: '''Unreleased structure''' The entry 6g2f is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6g2f is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]