6g6s - Revision history

OCA at 22:14, 9 August 2018

OCA — Thu, 09 Aug 2018 22:14:09 GMT

←Older revision		Revision as of 22:14, 9 August 2018
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	<StructureSection load='6g6s' size='340' side='right' caption='[[6g6s]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='6g6s' size='340' side='right' caption='[[6g6s]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6g6s]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G6S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G6S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6g6s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G6S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G6S FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g6s OCA], [http://pdbe.org/6g6s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g6s RCSB], [http://www.ebi.ac.uk/pdbsum/6g6s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g6s ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACIN1, ACINUS, KIAA0670 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g6s OCA], [http://pdbe.org/6g6s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g6s RCSB], [http://www.ebi.ac.uk/pdbsum/6g6s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g6s ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/ACINU_HUMAN ACINU_HUMAN]] Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets; ACIN1 confers RNA-binding to the complex. The ASAP complex can inhibit RNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function. Induces apoptotic chromatin condensation after activation by CASP3. Regulates cyclin A1, but not cyclin A2, expression in leukemia cells.<ref>PMID:10490026</ref> <ref>PMID:12665594</ref> <ref>PMID:18559500</ref> <ref>PMID:22203037</ref> <ref>PMID:22388736</ref>		[[http://www.uniprot.org/uniprot/ACINU_HUMAN ACINU_HUMAN]] Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets; ACIN1 confers RNA-binding to the complex. The ASAP complex can inhibit RNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function. Induces apoptotic chromatin condensation after activation by CASP3. Regulates cyclin A1, but not cyclin A2, expression in leukemia cells.<ref>PMID:10490026</ref> <ref>PMID:12665594</ref> <ref>PMID:18559500</ref> <ref>PMID:22203037</ref> <ref>PMID:22388736</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Acinus is an abundant nuclear protein involved in apoptosis and splicing. It has been implicated in inducing apoptotic chromatin condensation and DNA fragmentation during programmed cell death. Acinus undergoes activation by proteolytic cleavage that produces a truncated p17 form that comprises only the RNA recognition motif (RRM) domain. We have determined the crystal structure of the human Acinus RRM domain (AcRRM) at 1.65 A resolution. It shows a classical four-stranded antiparallel beta-sheet fold with two flanking alpha-helices and an additional, non-classical alpha-helix at the C-terminus, which harbors the caspase-3 target sequence that is cleaved during Acinus activation. In the structure, the C-terminal alpha-helix partially occludes the potential ligand binding surface of the beta-sheet and hypothetically shields it from non-sequence specific interactions with RNA. Based on the comparison with other RRM-RNA complex structures, it is likely that the C-terminal alpha-helix changes its conformation with respect to the RRM core in order to enable RNA binding by Acinus.
		+
		+	Crystal structure of human Acinus RNA recognition motif domain.,Fernandes H, Czapinska H, Grudziaz K, Bujnicki JM, Nowacka M PeerJ. 2018 Jul 4;6:e5163. doi: 10.7717/peerj.5163. eCollection 2018. PMID:30042883<ref>PMID:30042883</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6g6s" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Bujnicki, J M]]		[[Category: Bujnicki, J M]]
	[[Category: Czapinska, H]]		[[Category: Czapinska, H]]

OCA at 05:40, 27 June 2018

OCA — Wed, 27 Jun 2018 05:40:52 GMT

←Older revision		Revision as of 05:40, 27 June 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6g6s ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of human Acinus RNA recognition motif domain==
-		+	<StructureSection load='6g6s' size='340' side='right' caption='[[6g6s]], [[Resolution\|resolution]] 1.65Å' scene=''>
-	~~Authors~~: ~~Fernandes~~, H., ~~Czapinska, H~~., ~~Grudziaz, K~~., ~~Bujnicki, J~~.M., ~~Nowacka, M~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6g6s]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G6S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G6S FirstGlance]. <br>
-	~~Description~~: ~~Crystal~~ structure of ~~human Acinus~~ RNA ~~recognition motif domain~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g6s OCA], [http://pdbe.org/6g6s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g6s RCSB], [http://www.ebi.ac.uk/pdbsum/6g6s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g6s ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ACINU_HUMAN ACINU_HUMAN]] Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets; ACIN1 confers RNA-binding to the complex. The ASAP complex can inhibit RNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function. Induces apoptotic chromatin condensation after activation by CASP3. Regulates cyclin A1, but not cyclin A2, expression in leukemia cells.<ref>PMID:10490026</ref> <ref>PMID:12665594</ref> <ref>PMID:18559500</ref> <ref>PMID:22203037</ref> <ref>PMID:22388736</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bujnicki, J M]]
		+	[[Category: Czapinska, H]]
	[[Category: Fernandes, H]]		[[Category: Fernandes, H]]
		+	[[Category: Grudziaz, K]]
	[[Category: Nowacka, M]]		[[Category: Nowacka, M]]
-	[[Category: ~~Bujnicki, J.M~~]]	+	[[Category: Acinus]]
-	[[Category: ~~Czapinska, H~~]]	+	[[Category: Apoptosis]]
-	[[Category: ~~Grudziaz, K~~]]	+	[[Category: Rna binding protein]]
		+	[[Category: Rrm domain]]
		+	[[Category: Splicing factor]]

OCA at 04:16, 25 April 2018

OCA — Wed, 25 Apr 2018 04:16:49 GMT

←Older revision		Revision as of 04:16, 25 April 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6g6s is ON HOLD	+	The entry 6g6s is ON HOLD until Paper Publication

	Authors: Fernandes, H., Czapinska, H., Grudziaz, K., Bujnicki, J.M., Nowacka, M.		Authors: Fernandes, H., Czapinska, H., Grudziaz, K., Bujnicki, J.M., Nowacka, M.
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	Description: Crystal structure of human Acinus RNA recognition motif domain		Description: Crystal structure of human Acinus RNA recognition motif domain
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Czapinska, H]]
	[[Category: Fernandes, H]]		[[Category: Fernandes, H]]
	[[Category: Nowacka, M]]		[[Category: Nowacka, M]]
	[[Category: Bujnicki, J.M]]		[[Category: Bujnicki, J.M]]
		+	[[Category: Czapinska, H]]
	[[Category: Grudziaz, K]]		[[Category: Grudziaz, K]]

OCA: Protected "6g6s" [edit=sysop:move=sysop]

OCA — Wed, 11 Apr 2018 12:40:40 GMT

Protected "6g6s" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:40, 11 April 2018

OCA: New page: '''Unreleased structure''' The entry 6g6s is ON HOLD Authors: Fernandes, H., Czapinska, H., Grudziaz, K., Bujnicki, J.M., Nowacka, M. Description: Crystal structure of human Acinus RNA...

OCA — Wed, 11 Apr 2018 12:40:39 GMT

New page: '''Unreleased structure''' The entry 6g6s is ON HOLD Authors: Fernandes, H., Czapinska, H., Grudziaz, K., Bujnicki, J.M., Nowacka, M. Description: Crystal structure of human Acinus RNA...

New page

'''Unreleased structure'''

The entry 6g6s is ON HOLD

Authors: Fernandes, H., Czapinska, H., Grudziaz, K., Bujnicki, J.M., Nowacka, M.

Description: Crystal structure of human Acinus RNA recognition motif domain
[[Category: Unreleased Structures]]
[[Category: Czapinska, H]]
[[Category: Fernandes, H]]
[[Category: Nowacka, M]]
[[Category: Bujnicki, J.M]]
[[Category: Grudziaz, K]]