6gaq - Revision history

OCA at 20:22, 19 September 2018

OCA — Wed, 19 Sep 2018 20:22:57 GMT

←Older revision		Revision as of 20:22, 19 September 2018
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	<StructureSection load='6gaq' size='340' side='right' caption='[[6gaq]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='6gaq' size='340' side='right' caption='[[6gaq]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6gaq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GAQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GAQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6gaq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baccr Baccr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GAQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GAQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BC_3541 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=226900 BACCR])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gaq OCA], [http://pdbe.org/6gaq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gaq RCSB], [http://www.ebi.ac.uk/pdbsum/6gaq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gaq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gaq OCA], [http://pdbe.org/6gaq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gaq RCSB], [http://www.ebi.ac.uk/pdbsum/6gaq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gaq ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Baccr]]
	[[Category: Gudim, I]]		[[Category: Gudim, I]]
	[[Category: Hersleth, H P]]		[[Category: Hersleth, H P]]

OCA at 10:12, 5 September 2018

OCA — Wed, 05 Sep 2018 10:12:55 GMT

←Older revision		Revision as of 10:12, 5 September 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6gaq is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of oxidised Flavodoxin 2 from Bacillus cereus==
		+	<StructureSection load='6gaq' size='340' side='right' caption='[[6gaq]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6gaq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GAQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GAQ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gaq OCA], [http://pdbe.org/6gaq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gaq RCSB], [http://www.ebi.ac.uk/pdbsum/6gaq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gaq ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Flavodoxins (Flds) are small, bacterial proteins that transfer electrons to various redox enzymes. Flavodoxins are reduced by ferredoxin/flavodoxin NADP(+) oxidoreductases (FNRs), but little is known of the FNR-Fld interaction. Here, we compare the interactions of two flavodoxins (Fld1-2), one flavodoxin-like protein (NrdI), and three different thioredoxin reductase (TrxR)-like FNRs (FNR1-3), all from Bacillus cereus. Steady-state kinetics shows that the FNR2-Fld2 electron transfer pair is particularly efficient, and redox potential measurements also indicate that this is the most favorable electron donor/acceptor pair. Furthermore, crystal structures of FNR1 and FNR2 show that the proteins have crystallized in different conformations, a closed and an open conformation, respectively. We suggest that a large-scale conformational rearrangement takes place during the FNR catalytic cycle to allow for the binding and reduction of the Fld and, subsequently, the re-reduction of the FNR by NADPH. Finally, inspection of the residues surrounding the FAD cofactor in the FNR active site shows that a key isoalloxazine ring-stacking residue is different in FNR1 and FNR2, which could explain the large difference in catalytic efficiency between the two FNRs. To date, all the characterized TrxR-like FNRs have a residue with aromatic character stacking against the FAD isoalloxazine ring, and this has been thought to be a conserved feature of this class of FNRs. FNR1, however, has a valine in this position. Bioinformatic analysis shows that the TrxR-like FNRs can actually be divided into two groups, one group where the FAD-stacking residue has aromatic character and another group where it is valine.

-	~~Authors: Lofstad, M~~., Gudim, I., ~~Hersleth~~, H.-P.	+	Characterization of different flavodoxin reductase-flavodoxin (FNR-Fld) interactions reveals an efficient FNR-Fld redox pair and identifies a novel FNR subclass.,Gudim I, Hammerstad M, Lofstad M, Hersleth HP Biochemistry. 2018 Aug 24. doi: 10.1021/acs.biochem.8b00674. PMID:30142264<ref>PMID:30142264</ref>

-	~~Description: Crystal structure~~ of ~~oxidised Flavodoxin 2 from Bacillus cereus~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 6gaq" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Gudim, I]]		[[Category: Gudim, I]]
		+	[[Category: Hersleth, H P]]
	[[Category: Lofstad, M]]		[[Category: Lofstad, M]]
-	[[Category: ~~Hersleth, H.-P~~]]	+	[[Category: Electron transfer]]
		+	[[Category: Electron transport]]
		+	[[Category: Flavodoxin]]
		+	[[Category: Fmn]]

OCA at 05:01, 9 May 2018

OCA — Wed, 09 May 2018 05:01:56 GMT

←Older revision		Revision as of 05:01, 9 May 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6gaq is ON HOLD	+	The entry 6gaq is ON HOLD until Paper Publication

	Authors: Lofstad, M., Gudim, I., Hersleth, H.-P.		Authors: Lofstad, M., Gudim, I., Hersleth, H.-P.

OCA: Protected "6gaq" [edit=sysop:move=sysop]

OCA — Wed, 18 Apr 2018 04:34:06 GMT

Protected "6gaq" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:34, 18 April 2018

OCA: New page: '''Unreleased structure''' The entry 6gaq is ON HOLD Authors: Lofstad, M., Gudim, I., Hersleth, H.-P. Description: Crystal structure of oxidised Flavodoxin 2 from Bacillus cereus [[Cat...

OCA — Wed, 18 Apr 2018 04:34:05 GMT

New page: '''Unreleased structure''' The entry 6gaq is ON HOLD Authors: Lofstad, M., Gudim, I., Hersleth, H.-P. Description: Crystal structure of oxidised Flavodoxin 2 from Bacillus cereus [[Cat...

New page

'''Unreleased structure'''

The entry 6gaq is ON HOLD

Authors: Lofstad, M., Gudim, I., Hersleth, H.-P.

Description: Crystal structure of oxidised Flavodoxin 2 from Bacillus cereus
[[Category: Unreleased Structures]]
[[Category: Gudim, I]]
[[Category: Lofstad, M]]
[[Category: Hersleth, H.-P]]