6gar - Revision history

OCA at 20:22, 19 September 2018

OCA — Wed, 19 Sep 2018 20:22:59 GMT

←Older revision		Revision as of 20:22, 19 September 2018
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	<StructureSection load='6gar' size='340' side='right' caption='[[6gar]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='6gar' size='340' side='right' caption='[[6gar]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6gar]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GAR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GAR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6gar]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baccr Baccr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GAR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GAR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=OXM:OXAMIC+ACID'>OXM</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=OXM:OXAMIC+ACID'>OXM</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BC_0385 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=226900 BACCR])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gar OCA], [http://pdbe.org/6gar PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gar RCSB], [http://www.ebi.ac.uk/pdbsum/6gar PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gar ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gar OCA], [http://pdbe.org/6gar PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gar RCSB], [http://www.ebi.ac.uk/pdbsum/6gar PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gar ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Baccr]]
	[[Category: Gudim, I]]		[[Category: Gudim, I]]
	[[Category: Hersleth, H P]]		[[Category: Hersleth, H P]]

OCA at 10:12, 5 September 2018

OCA — Wed, 05 Sep 2018 10:12:59 GMT

←Older revision		Revision as of 10:12, 5 September 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6gar is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of oxidised ferredoxin/flavodoxin NADP+ oxidoreductase 1 (FNR1) from Bacillus cereus==
		+	<StructureSection load='6gar' size='340' side='right' caption='[[6gar]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6gar]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GAR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GAR FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=OXM:OXAMIC+ACID'>OXM</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gar OCA], [http://pdbe.org/6gar PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gar RCSB], [http://www.ebi.ac.uk/pdbsum/6gar PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gar ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Flavodoxins (Flds) are small, bacterial proteins that transfer electrons to various redox enzymes. Flavodoxins are reduced by ferredoxin/flavodoxin NADP(+) oxidoreductases (FNRs), but little is known of the FNR-Fld interaction. Here, we compare the interactions of two flavodoxins (Fld1-2), one flavodoxin-like protein (NrdI), and three different thioredoxin reductase (TrxR)-like FNRs (FNR1-3), all from Bacillus cereus. Steady-state kinetics shows that the FNR2-Fld2 electron transfer pair is particularly efficient, and redox potential measurements also indicate that this is the most favorable electron donor/acceptor pair. Furthermore, crystal structures of FNR1 and FNR2 show that the proteins have crystallized in different conformations, a closed and an open conformation, respectively. We suggest that a large-scale conformational rearrangement takes place during the FNR catalytic cycle to allow for the binding and reduction of the Fld and, subsequently, the re-reduction of the FNR by NADPH. Finally, inspection of the residues surrounding the FAD cofactor in the FNR active site shows that a key isoalloxazine ring-stacking residue is different in FNR1 and FNR2, which could explain the large difference in catalytic efficiency between the two FNRs. To date, all the characterized TrxR-like FNRs have a residue with aromatic character stacking against the FAD isoalloxazine ring, and this has been thought to be a conserved feature of this class of FNRs. FNR1, however, has a valine in this position. Bioinformatic analysis shows that the TrxR-like FNRs can actually be divided into two groups, one group where the FAD-stacking residue has aromatic character and another group where it is valine.

-	~~Authors: Skramo, S~~., Gudim, I., ~~Hersleth~~, H.-P.	+	Characterization of different flavodoxin reductase-flavodoxin (FNR-Fld) interactions reveals an efficient FNR-Fld redox pair and identifies a novel FNR subclass.,Gudim I, Hammerstad M, Lofstad M, Hersleth HP Biochemistry. 2018 Aug 24. doi: 10.1021/acs.biochem.8b00674. PMID:30142264<ref>PMID:30142264</ref>

-	~~Description: Crystal structure~~ of ~~oxidised ferredoxin~~/~~flavodoxin NADP+ oxidoreductase 1 (FNR1) from Bacillus cereus~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 6gar" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Gudim, I]]		[[Category: Gudim, I]]
		+	[[Category: Hersleth, H P]]
	[[Category: Skramo, S]]		[[Category: Skramo, S]]
-	[[Category: ~~Hersleth, H.-P~~]]	+	[[Category: Electron transfer]]
		+	[[Category: Fad]]
		+	[[Category: Ferredoxin/flavodoxin reductase]]
		+	[[Category: Flavoprotein]]
		+	[[Category: Oxidoreductase]]

OCA at 05:01, 9 May 2018

OCA — Wed, 09 May 2018 05:01:59 GMT

←Older revision		Revision as of 05:01, 9 May 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6gar is ON HOLD	+	The entry 6gar is ON HOLD until Paper Publication

-	Authors: ~~Skraamo~~, S., Gudim, I., Hersleth, H.-P.	+	Authors: Skramo, S., Gudim, I., Hersleth, H.-P.

	Description: Crystal structure of oxidised ferredoxin/flavodoxin NADP+ oxidoreductase 1 (FNR1) from Bacillus cereus		Description: Crystal structure of oxidised ferredoxin/flavodoxin NADP+ oxidoreductase 1 (FNR1) from Bacillus cereus
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Gudim, I]]		[[Category: Gudim, I]]
		+	[[Category: Skramo, S]]
	[[Category: Hersleth, H.-P]]		[[Category: Hersleth, H.-P]]
-	[[Category: Skraamo, S]]

OCA: Protected "6gar" [edit=sysop:move=sysop]

OCA — Wed, 18 Apr 2018 04:34:11 GMT

Protected "6gar" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:34, 18 April 2018

OCA: New page: '''Unreleased structure''' The entry 6gar is ON HOLD Authors: Skraamo, S., Gudim, I., Hersleth, H.-P. Description: Crystal structure of oxidised ferredoxin/flavodoxin NADP+ oxidoreduct...

OCA — Wed, 18 Apr 2018 04:34:09 GMT

New page: '''Unreleased structure''' The entry 6gar is ON HOLD Authors: Skraamo, S., Gudim, I., Hersleth, H.-P. Description: Crystal structure of oxidised ferredoxin/flavodoxin NADP+ oxidoreduct...

New page

'''Unreleased structure'''

The entry 6gar is ON HOLD

Authors: Skraamo, S., Gudim, I., Hersleth, H.-P.

Description: Crystal structure of oxidised ferredoxin/flavodoxin NADP+ oxidoreductase 1 (FNR1) from Bacillus cereus
[[Category: Unreleased Structures]]
[[Category: Gudim, I]]
[[Category: Hersleth, H.-P]]
[[Category: Skraamo, S]]