6gbp - Revision history

OCA at 12:35, 9 May 2024

OCA — Thu, 09 May 2024 12:35:04 GMT

←Older revision		Revision as of 12:35, 9 May 2024
Line 1:		Line 1:

	==Crystal Structure of the oligomerization domain of VP35 from Ebola virus, mercury derivative==		==Crystal Structure of the oligomerization domain of VP35 from Ebola virus, mercury derivative==
-	<StructureSection load='6gbp' size='340' side='right' caption='[[6gbp]], [[Resolution\|resolution]] 3.49Å' scene=''>	+	<StructureSection load='6gbp' size='340' side='right'caption='[[6gbp]], [[Resolution\|resolution]] 3.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6gbp]] is a 12 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Zebov Zebov~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GBP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6GBP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6gbp]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Zaire_ebolavirus Zaire ebolavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GBP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GBP FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=HG:MERCURY+(II)+ION'>HG</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.49Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~VP35 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=186538 ZEBOV]~~)</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6gbp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gbp OCA], [~~http~~://pdbe.org/6gbp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6gbp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6gbp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6gbp ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gbp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gbp OCA], [https://pdbe.org/6gbp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gbp RCSB], [https://www.ebi.ac.uk/pdbsum/6gbp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gbp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/VP35_EBOZM VP35_EBOZM]] Acsts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. The mechanism by which this blockage occurs remains incompletely defined, a hypothesis suggests that VP35 dsRNA-binding activity prevents activation of IRF3 by sequestering dsRNA. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR.<ref>PMID:9971816</ref> <ref>PMID:11027311</ref> <ref>PMID:12829834</ref> <ref>PMID:16495261</ref> <ref>PMID:17065211</ref>	+	[https://www.uniprot.org/uniprot/VP35_EBOZM VP35_EBOZM] Acsts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. The mechanism by which this blockage occurs remains incompletely defined, a hypothesis suggests that VP35 dsRNA-binding activity prevents activation of IRF3 by sequestering dsRNA. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR.<ref>PMID:9971816</ref> <ref>PMID:11027311</ref> <ref>PMID:12829834</ref> <ref>PMID:16495261</ref> <ref>PMID:17065211</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Zebov~~]]	+	[[Category: Large Structures]]
-	[[Category: Baumeister, W]]	+	[[Category: Zaire ebolavirus]]
-	[[Category: Bracher, A]]	+	[[Category: Baumeister W]]
-	[[Category: Nagy, I]]	+	[[Category: Bracher A]]
-	[[Category: Orsini, M]]	+	[[Category: Nagy I]]
-	[[Category: Weyher-Stingl, E]]	+	[[Category: Orsini M]]
-	[[Category: Zinzula, L]]	+	[[Category: Weyher-Stingl E]]
-	~~[[Category: Coiled-coil]]~~	+	[[Category: Zinzula L]]
-	~~[[Category: Viral protein~~]]	+

OCA at 07:17, 12 December 2018

OCA — Wed, 12 Dec 2018 07:17:22 GMT

←Older revision		Revision as of 07:17, 12 December 2018
Line 3:		Line 3:
	<StructureSection load='6gbp' size='340' side='right' caption='[[6gbp]], [[Resolution\|resolution]] 3.49Å' scene=''>		<StructureSection load='6gbp' size='340' side='right' caption='[[6gbp]], [[Resolution\|resolution]] 3.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6gbp]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GBP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GBP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6gbp]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Zebov Zebov]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GBP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GBP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VP35 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186538 ZEBOV])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gbp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gbp OCA], [http://pdbe.org/6gbp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gbp RCSB], [http://www.ebi.ac.uk/pdbsum/6gbp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gbp ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gbp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gbp OCA], [http://pdbe.org/6gbp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gbp RCSB], [http://www.ebi.ac.uk/pdbsum/6gbp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gbp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/VP35_EBOZM VP35_EBOZM]] Acsts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. The mechanism by which this blockage occurs remains incompletely defined, a hypothesis suggests that VP35 dsRNA-binding activity prevents activation of IRF3 by sequestering dsRNA. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR.<ref>PMID:9971816</ref> <ref>PMID:11027311</ref> <ref>PMID:12829834</ref> <ref>PMID:16495261</ref> <ref>PMID:17065211</ref>		[[http://www.uniprot.org/uniprot/VP35_EBOZM VP35_EBOZM]] Acsts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. The mechanism by which this blockage occurs remains incompletely defined, a hypothesis suggests that VP35 dsRNA-binding activity prevents activation of IRF3 by sequestering dsRNA. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR.<ref>PMID:9971816</ref> <ref>PMID:11027311</ref> <ref>PMID:12829834</ref> <ref>PMID:16495261</ref> <ref>PMID:17065211</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The multifunctional virion protein 35 (VP35) of ebolaviruses is a critical determinant of virulence and pathogenesis indispensable for viral replication and host innate immune evasion. Essential for VP35 function is homo-oligomerization via a coiled-coil motif. Here we report crystal structures of VP35 oligomerization domains from the prototypic Ebola virus (EBOV) and the non-pathogenic Reston virus (RESTV), together with a comparative biophysical characterization of the domains from all known species of the Ebolavirus genus. EBOV and RESTV VP35 oligomerization domains form bipartite parallel helix bundles with a canonical coiled coil in the N-terminal half and increased plasticity in the highly conserved C-terminal half. The domain assembles into trimers and tetramers in EBOV, whereas it exclusively forms tetramers in all other ebolavirus species. Substitution of coiled-coil leucine residues critical for immune antagonism leads to aberrant oligomerization. A conserved arginine involved in inter-chain salt bridges stabilizes the VP35 oligomerization domain and modulates between coiled-coil oligomeric states.
		+
		+	Structures of Ebola and Reston Virus VP35 Oligomerization Domains and Comparative Biophysical Characterization in All Ebolavirus Species.,Zinzula L, Nagy I, Orsini M, Weyher-Stingl E, Bracher A, Baumeister W Structure. 2018 Oct 5. pii: S0969-2126(18)30335-6. doi:, 10.1016/j.str.2018.09.009. PMID:30482729<ref>PMID:30482729</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6gbp" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Zebov]]
	[[Category: Baumeister, W]]		[[Category: Baumeister, W]]
	[[Category: Bracher, A]]		[[Category: Bracher, A]]

OCA at 08:13, 10 October 2018

OCA — Wed, 10 Oct 2018 08:13:15 GMT

←Older revision		Revision as of 08:13, 10 October 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6gbp is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Crystal Structure of the oligomerization domain of VP35 from Ebola virus, mercury derivative==
-		+	<StructureSection load='6gbp' size='340' side='right' caption='[[6gbp]], [[Resolution\|resolution]] 3.49Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6gbp]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GBP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GBP FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gbp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gbp OCA], [http://pdbe.org/6gbp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gbp RCSB], [http://www.ebi.ac.uk/pdbsum/6gbp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gbp ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/VP35_EBOZM VP35_EBOZM]] Acsts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. The mechanism by which this blockage occurs remains incompletely defined, a hypothesis suggests that VP35 dsRNA-binding activity prevents activation of IRF3 by sequestering dsRNA. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR.<ref>PMID:9971816</ref> <ref>PMID:11027311</ref> <ref>PMID:12829834</ref> <ref>PMID:16495261</ref> <ref>PMID:17065211</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Baumeister, W]]
		+	[[Category: Bracher, A]]
		+	[[Category: Nagy, I]]
		+	[[Category: Orsini, M]]
		+	[[Category: Weyher-Stingl, E]]
		+	[[Category: Zinzula, L]]
		+	[[Category: Coiled-coil]]
		+	[[Category: Viral protein]]

OCA: Protected "6gbp" [edit=sysop:move=sysop]

OCA — Wed, 25 Apr 2018 04:19:44 GMT

Protected "6gbp" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:19, 25 April 2018

OCA: New page: '''Unreleased structure''' The entry 6gbp is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 25 Apr 2018 04:19:42 GMT

New page: '''Unreleased structure''' The entry 6gbp is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6gbp is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]