6geb - Revision history

OCA at 06:19, 11 July 2018

OCA — Wed, 11 Jul 2018 06:19:42 GMT

←Older revision		Revision as of 06:19, 11 July 2018
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	<StructureSection load='6geb' size='340' side='right' caption='[[6geb]], [[Resolution\|resolution]] 3.19Å' scene=''>		<StructureSection load='6geb' size='340' side='right' caption='[[6geb]], [[Resolution\|resolution]] 3.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6geb]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GEB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GEB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6geb]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33152 Atcc 33152]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GEB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GEB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dotB, yggR, ERS240541_01758, ERS240560_02271, ERS253249_00407 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=446 ATCC 33152])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6geb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6geb OCA], [http://pdbe.org/6geb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6geb RCSB], [http://www.ebi.ac.uk/pdbsum/6geb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6geb ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6geb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6geb OCA], [http://pdbe.org/6geb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6geb RCSB], [http://www.ebi.ac.uk/pdbsum/6geb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6geb ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 33152]]
	[[Category: Prevost, M S]]		[[Category: Prevost, M S]]
	[[Category: Waksman, G]]		[[Category: Waksman, G]]
	[[Category: Aaa+ atpase type ivb secretion]]		[[Category: Aaa+ atpase type ivb secretion]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 05:40, 30 May 2018

OCA — Wed, 30 May 2018 05:40:51 GMT

←Older revision		Revision as of 05:40, 30 May 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6geb is ~~ON HOLD~~	+	==X-ray structure of the Legionella pneumophila ATPase DotB==
		+	<StructureSection load='6geb' size='340' side='right' caption='[[6geb]], [[Resolution\|resolution]] 3.19Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6geb]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GEB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GEB FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6geb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6geb OCA], [http://pdbe.org/6geb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6geb RCSB], [http://www.ebi.ac.uk/pdbsum/6geb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6geb ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Human infections by the intracellular bacterial pathogen Legionella pneumophila result in a severe form of pneumonia, the Legionnaire's disease. L. pneumophila utilises a type IVb secretion (T4bS) system termed "dot/icm" to secrete protein effectors to the host cytoplasm. The dot/icm system is powered at least in part by a functionally critical AAA+ ATPase, a protein called DotB, thought to belong to the VirB11 family of proteins. Here we present the crystal structure of DotB at 3.19 A resolution, in its hexameric form. We observe that DotB is in fact a structural intermediate between VirB11 and PilT family proteins, with a PAS-like N-terminal domain coupled to a RecA-like C-terminal domain. It also shares critical structural elements only found in PilT. The structure also reveals two conformers, termed alpha and beta, with an alphabetaalphabetaalphabeta configuration. The existence of alpha and beta conformers in this class of proteins was confirmed by solving the structure of DotB from another bacterial pathogen, Yersinia, where, intriguingly, we observed an alphaalphabetaalphaalphabeta configuration. The two conformers co-exist regardless of the nucleotide-bound states of the proteins. Our investigation therefore reveals that these ATPases can adopt a wider range of conformational states than was known before, shedding new light on the extraordinary spectrum of conformations these ATPases can access to carry out their function. Overall, the structure of DotB provides a template for further rational drug-design to develop more specific antibiotics to tackle Legionnaire's disease. This article is protected by copyright. All rights reserved.

-	~~Authors: Prevost, M.S~~., ~~Waksman~~, G.	+	X-ray Crystal Structures of the Type IVb Secretion System DotB ATPases.,Prevost MS, Waksman G Protein Sci. 2018 May 17. doi: 10.1002/pro.3439. PMID:29770512<ref>PMID:29770512</ref>

-	~~Description: X-ray structure~~ of the ~~Legionella pneumophila ATPase DotB~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: Prevost, M.S]]	+	<div class="pdbe-citations 6geb" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Prevost, M S]]
	[[Category: Waksman, G]]		[[Category: Waksman, G]]
		+	[[Category: Aaa+ atpase type ivb secretion]]
		+	[[Category: Hydrolase]]

OCA: Protected "6geb" [edit=sysop:move=sysop]

OCA — Wed, 02 May 2018 06:56:11 GMT

Protected "6geb" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:56, 2 May 2018

OCA: New page: '''Unreleased structure''' The entry 6geb is ON HOLD Authors: Prevost, M.S., Waksman, G. Description: X-ray structure of the Legionella pneumophila ATPase DotB [[Category: Unreleased S...

OCA — Wed, 02 May 2018 06:56:09 GMT

New page: '''Unreleased structure''' The entry 6geb is ON HOLD Authors: Prevost, M.S., Waksman, G. Description: X-ray structure of the Legionella pneumophila ATPase DotB [[Category: Unreleased S...

New page

'''Unreleased structure'''

The entry 6geb is ON HOLD

Authors: Prevost, M.S., Waksman, G.

Description: X-ray structure of the Legionella pneumophila ATPase DotB
[[Category: Unreleased Structures]]
[[Category: Prevost, M.S]]
[[Category: Waksman, G]]