OCA at 05:51, 21 August 2019

OCA — Wed, 21 Aug 2019 05:51:46 GMT

←Older revision		Revision as of 05:51, 21 August 2019
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-	'''Unreleased structure'''

-	The ~~entry~~ 6gel is ~~ON HOLD until Paper~~ Publication	+	==The structure of TWITCH-2B==
		+	<StructureSection load='6gel' size='340' side='right'caption='[[6gel]], [[Resolution\|resolution]] 2.51Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6gel]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GEL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GEL FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene>, <scene name='pdbligand=CRF:[(4Z)-2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(1H-INDOL-3-YLMETHYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>CRF</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gel OCA], [http://pdbe.org/6gel PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gel RCSB], [http://www.ebi.ac.uk/pdbsum/6gel PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gel ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The quality of genetically encoded calcium indicators (GECIs) has improved dramatically in recent years, but high-performing ratiometric indicators are still rare. Here we describe a series of fluorescence resonance energy transfer (FRET)-based calcium biosensors with a reduced number of calcium binding sites per sensor. These 'Twitch' sensors are based on the C-terminal domain of Opsanus troponin C. Their FRET responses were optimized by a large-scale functional screen in bacterial colonies, refined by a secondary screen in rat hippocampal neuron cultures. We tested the in vivo performance of the most sensitive variants in the brain and lymph nodes of mice. The sensitivity of the Twitch sensors matched that of synthetic calcium dyes and allowed visualization of tonic action potential firing in neurons and high resolution functional tracking of T lymphocytes. Given their ratiometric readout, their brightness, large dynamic range and linear response properties, Twitch sensors represent versatile tools for neuroscience and immunology.

-	~~Authors~~:	+	Optimized ratiometric calcium sensors for functional in vivo imaging of neurons and T lymphocytes.,Thestrup T, Litzlbauer J, Bartholomaus I, Mues M, Russo L, Dana H, Kovalchuk Y, Liang Y, Kalamakis G, Laukat Y, Becker S, Witte G, Geiger A, Allen T, Rome LC, Chen TW, Kim DS, Garaschuk O, Griesinger C, Griesbeck O Nat Methods. 2014 Feb;11(2):175-82. doi: 10.1038/nmeth.2773. Epub 2014 Jan 5. PMID:24390440<ref>PMID:24390440</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6gel" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Becker, S]]
		+	[[Category: Griesbeck, O]]
		+	[[Category: Griesinger, C]]
		+	[[Category: Mourino, P Trigo]]
		+	[[Category: Paulat, M]]
		+	[[Category: Thestrup, T]]
		+	[[Category: Fluorescent protein]]
		+	[[Category: Fret]]
		+	[[Category: Ratiometric biosensor]]
		+	[[Category: Twitch-2b]]

OCA at 05:49, 14 June 2018

OCA — Thu, 14 Jun 2018 05:49:53 GMT

←Older revision		Revision as of 05:49, 14 June 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6gel is ON HOLD	+	The entry 6gel is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6gel" [edit=sysop:move=sysop]

OCA — Wed, 09 May 2018 05:03:26 GMT

Protected "6gel" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:03, 9 May 2018

OCA: New page: '''Unreleased structure''' The entry 6gel is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 09 May 2018 05:03:25 GMT

New page: '''Unreleased structure''' The entry 6gel is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6gel is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6gel - Revision history

OCA at 05:51, 21 August 2019

OCA at 05:49, 14 June 2018

OCA: Protected "6gel" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6gel is ON HOLD Authors: Description: Category: Unreleased Structures