6gfg - Revision history

OCA at 07:18, 31 October 2018

OCA — Wed, 31 Oct 2018 07:18:29 GMT

←Older revision		Revision as of 07:18, 31 October 2018
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	<StructureSection load='6gfg' size='340' side='right' caption='[[6gfg]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='6gfg' size='340' side='right' caption='[[6gfg]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6gfg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GFG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GFG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6gfg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GFG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GFG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=KGN:D-chiro+inositol+hexakisphosphate'>KGN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=KGN:D-chiro+inositol+hexakisphosphate'>KGN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AXX17_At5g40720 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inositol-pentakisphosphate_2-kinase Inositol-pentakisphosphate 2-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.158 2.7.1.158] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inositol-pentakisphosphate_2-kinase Inositol-pentakisphosphate 2-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.158 2.7.1.158] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gfg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gfg OCA], [http://pdbe.org/6gfg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gfg RCSB], [http://www.ebi.ac.uk/pdbsum/6gfg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gfg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gfg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gfg OCA], [http://pdbe.org/6gfg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gfg RCSB], [http://www.ebi.ac.uk/pdbsum/6gfg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gfg ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arath]]
	[[Category: Inositol-pentakisphosphate 2-kinase]]		[[Category: Inositol-pentakisphosphate 2-kinase]]
	[[Category: Brearley, C A]]		[[Category: Brearley, C A]]

OCA at 07:39, 12 September 2018

OCA — Wed, 12 Sep 2018 07:39:36 GMT

←Older revision		Revision as of 07:39, 12 September 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6gfg is ~~ON HOLD~~	+	==Inositol 1,3,4,5,6-pentakisphosphate 2-kinase from A. thaliana in complex with D-chiro-IP6 and ADP==
		+	<StructureSection load='6gfg' size='340' side='right' caption='[[6gfg]], [[Resolution\|resolution]] 3.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6gfg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GFG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GFG FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=KGN:D-chiro+inositol+hexakisphosphate'>KGN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inositol-pentakisphosphate_2-kinase Inositol-pentakisphosphate 2-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.158 2.7.1.158] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gfg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gfg OCA], [http://pdbe.org/6gfg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gfg RCSB], [http://www.ebi.ac.uk/pdbsum/6gfg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gfg ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/A0A178UAB5_ARATH A0A178UAB5_ARATH]] Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate).[RuleBase:RU364126]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Inositol pentakisphosphate 2-kinase catalyzes the phosphorylation of the axial 2-OH of myo-inositol 1,3,4,5,6-pentakisphosphate for de novo synthesis of myo-inositol hexakisphosphate. Disruption of inositol pentakisphosphate 2-kinase profoundly influences cellular processes; from nuclear mRNA export and phosphate homeostasis in yeast and plants, to establishment of left-right asymmetry in zebra fish. We elaborate an active site fluorescent probe that allows high throughput screening of Arabidopsis inositol pentakisphosphate 2-kinase. We show that the probe has a binding constant comparable to the Km values of inositol phosphate substrates of this enzyme, and can be used to prospect for novel substrates and inhibitors of inositol phosphate kinases. We identify several micromolar Ki inhibitors and validate this approach by solving the crystal structure of protein in complex with purpurogallin. We additionally solve structures of protein in complexes with epimeric higher inositol phosphates. This probe may find utility in characterization of a wide family of inositol phosphate kinases.

-	~~Authors: Whitfield~~, H~~.L.~~, ~~Brearley~~, ~~C.A.~~, Hemmings, A.M.	+	A Fluorescent Probe Identifies Active Site Ligands of Inositol Pentakisphosphate 2-Kinase.,Whitfield H, Gilmartin M, Baker K, Riley AM, Godage HY, Potter BVL, Hemmings AM, Brearley CA J Med Chem. 2018 Aug 30. doi: 10.1021/acs.jmedchem.8b01022. PMID:30160967<ref>PMID:30160967</ref>

-	~~Description: Inositol 1~~,~~3,4,5,6-pentakisphosphate 2-kinase from A~~. ~~thaliana in complex with D~~-~~chiro~~-~~IP6 and ADP~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Brearley, C.A]]	+	<div class="pdbe-citations 6gfg" style="background-color:#fffaf0;"></div>
-	[[Category: Hemmings, A.M]]	+	== References ==
-	[[Category: Whitfield, H.L]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Inositol-pentakisphosphate 2-kinase]]
		+	[[Category: Brearley, C A]]
		+	[[Category: Hemmings, A M]]
		+	[[Category: Whitfield, H L]]
		+	[[Category: Ipk1]]
		+	[[Category: Myo-ip5]]
		+	[[Category: Transferase]]

OCA: Protected "6gfg" [edit=sysop:move=sysop]

OCA — Wed, 09 May 2018 05:05:33 GMT

Protected "6gfg" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:05, 9 May 2018

OCA: New page: '''Unreleased structure''' The entry 6gfg is ON HOLD Authors: Whitfield, H.L., Brearley, C.A., Hemmings, A.M. Description: Inositol 1,3,4,5,6-pentakisphosphate 2-kinase from A. thalian...

OCA — Wed, 09 May 2018 05:05:31 GMT

New page: '''Unreleased structure''' The entry 6gfg is ON HOLD Authors: Whitfield, H.L., Brearley, C.A., Hemmings, A.M. Description: Inositol 1,3,4,5,6-pentakisphosphate 2-kinase from A. thalian...

New page

'''Unreleased structure'''

The entry 6gfg is ON HOLD

Authors: Whitfield, H.L., Brearley, C.A., Hemmings, A.M.

Description: Inositol 1,3,4,5,6-pentakisphosphate 2-kinase from A. thaliana in complex with D-chiro-IP6 and ADP
[[Category: Unreleased Structures]]
[[Category: Brearley, C.A]]
[[Category: Hemmings, A.M]]
[[Category: Whitfield, H.L]]