6gfk - Revision history

OCA at 08:54, 3 October 2018

OCA — Wed, 03 Oct 2018 08:54:26 GMT

←Older revision		Revision as of 08:54, 3 October 2018
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	<StructureSection load='6gfk' size='340' side='right' caption='[[6gfk]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='6gfk' size='340' side='right' caption='[[6gfk]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6gfk]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GFK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GFK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6gfk]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GFK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GFK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">METTL16, METT10D ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gfk OCA], [http://pdbe.org/6gfk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gfk RCSB], [http://www.ebi.ac.uk/pdbsum/6gfk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gfk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gfk OCA], [http://pdbe.org/6gfk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gfk RCSB], [http://www.ebi.ac.uk/pdbsum/6gfk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gfk ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Chen, K M]]		[[Category: Chen, K M]]
	[[Category: Homolka, D]]		[[Category: Homolka, D]]

OCA at 19:56, 19 September 2018

OCA — Wed, 19 Sep 2018 19:56:43 GMT

←Older revision		Revision as of 19:56, 19 September 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6gfk is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==delta-N METTL16 MTase domain==
		+	<StructureSection load='6gfk' size='340' side='right' caption='[[6gfk]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6gfk]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GFK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GFK FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gfk OCA], [http://pdbe.org/6gfk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gfk RCSB], [http://www.ebi.ac.uk/pdbsum/6gfk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gfk ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MET16_HUMAN MET16_HUMAN]] RNA N6-methyltransferase that methylates adenosine residues of a subset of RNAs and plays a key role in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts (PubMed:28525753). Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs) (PubMed:28525753). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure (PubMed:28525753). In presence of S-adenosyl-L-methionine, binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, impairing MAT2A expression (PubMed:28525753). In S-adenosyl-L-methionine-limiting conditions, binds the 3'-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, preventing N6-methylation and promoting expression of MAT2A (PubMed:28525753). In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA): specifically N6-methylates adenine in position 43 of U6 snRNAs (PubMed:28525753, PubMed:29051200). Also able to bind various lncRNAs (PubMed:29051200). Specifically binds the 3'-end of the MALAT1 long non-coding RNA (PubMed:27872311).<ref>PMID:27872311</ref> <ref>PMID:28525753</ref> <ref>PMID:29051200</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Internal modification of RNAs with N(6)-methyladenosine (m(6)A) is a highly conserved means of gene expression control. While the METTL3/METTL14 heterodimer adds this mark on thousands of transcripts in a single-stranded context, the substrate requirements and physiological roles of the second m(6)A writer METTL16 remain unknown. Here we describe the crystal structure of human METTL16 to reveal a methyltransferase domain furnished with an extra N-terminal module, which together form a deep-cut groove that is essential for RNA binding. When presented with a random pool of RNAs, METTL16 selects for methylation-structured RNAs where the critical adenosine is present in a bulge. Mouse 16-cell embryos lacking Mettl16 display reduced mRNA levels of its methylation target, the SAM synthetase Mat2a. The consequence is massive transcriptome dysregulation in approximately 64-cell blastocysts that are unfit for further development. This highlights the role of an m(6)A RNA methyltransferase in facilitating early development via regulation of SAM availability.

-	~~Authors: Chen, K.M~~., Mendel, M., ~~Homolka~~, D., McCarthy, A.A., ~~Pillai, R~~.S.	+	Methylation of Structured RNA by the m(6)A Writer METTL16 Is Essential for Mouse Embryonic Development.,Mendel M, Chen KM, Homolka D, Gos P, Pandey RR, McCarthy AA, Pillai RS Mol Cell. 2018 Sep 5. pii: S1097-2765(18)30638-5. doi:, 10.1016/j.molcel.2018.08.004. PMID:30197299<ref>PMID:30197299</ref>

-	~~Description: delta-N METTL16 MTase domain~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category: Chen~~, K.~~M]]~~	+	<div class="pdbe-citations 6gfk" style="background-color:#fffaf0;"></div>
-	~~[[Category: Pillai, R~~.~~S]]~~	+	== References ==
-	~~[[Category~~: ~~Mccarthy, A.A]]~~	+	<references/>
-	[[Category: ~~Mendel~~, M]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Chen, K M]]
	[[Category: Homolka, D]]		[[Category: Homolka, D]]
		+	[[Category: McCarthy, A A]]
		+	[[Category: Mendel, M]]
		+	[[Category: Pillai, R S]]
		+	[[Category: Sam methyltransferase]]
		+	[[Category: Transferase]]

OCA at 05:50, 4 July 2018

OCA — Wed, 04 Jul 2018 05:50:53 GMT

←Older revision		Revision as of 05:50, 4 July 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6gfk is ON HOLD	+	The entry 6gfk is ON HOLD until Paper Publication

	Authors: Chen, K.M., Mendel, M., Homolka, D., McCarthy, A.A., Pillai, R.S.		Authors: Chen, K.M., Mendel, M., Homolka, D., McCarthy, A.A., Pillai, R.S.

OCA: Protected "6gfk" [edit=sysop:move=sysop]

OCA — Wed, 09 May 2018 05:05:52 GMT

Protected "6gfk" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:05, 9 May 2018

OCA: New page: '''Unreleased structure''' The entry 6gfk is ON HOLD Authors: Chen, K.M., Mendel, M., Homolka, D., McCarthy, A.A., Pillai, R.S. Description: delta-N METTL16 MTase domain [[Category: Un...

OCA — Wed, 09 May 2018 05:05:51 GMT

New page: '''Unreleased structure''' The entry 6gfk is ON HOLD Authors: Chen, K.M., Mendel, M., Homolka, D., McCarthy, A.A., Pillai, R.S. Description: delta-N METTL16 MTase domain [[Category: Un...

New page

'''Unreleased structure'''

The entry 6gfk is ON HOLD

Authors: Chen, K.M., Mendel, M., Homolka, D., McCarthy, A.A., Pillai, R.S.

Description: delta-N METTL16 MTase domain
[[Category: Unreleased Structures]]
[[Category: Chen, K.M]]
[[Category: Pillai, R.S]]
[[Category: Mccarthy, A.A]]
[[Category: Mendel, M]]
[[Category: Homolka, D]]