6gh7 - Revision history

OCA at 05:58, 22 April 2020

OCA — Wed, 22 Apr 2020 05:58:32 GMT

←Older revision		Revision as of 05:58, 22 April 2020
Line 1:		Line 1:

-	==WILDTYPE CORE-STREPTAVIDIN WITH ~~BIOTIN A with~~ a ~~catalyst~~==	+	==WILDTYPE CORE-STREPTAVIDIN WITH a conjugated BIOTINYLATED PYRROLIDINE==
-	<StructureSection load='6gh7' size='340' side='right' caption='[[6gh7]], [[Resolution\|resolution]] 1.08Å' scene=''>	+	<StructureSection load='6gh7' size='340' side='right'caption='[[6gh7]], [[Resolution\|resolution]] 1.08Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6gh7]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GH7 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6GH7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6gh7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GH7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6GH7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EYW:5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-[(3~{R})-pyrrolidin-3-yl]pentanamide'>EYW</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EYW:5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-[(3~{R})-pyrrolidin-3-yl]pentanamide'>EYW</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6gh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gh7 OCA], [http://pdbe.org/6gh7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gh7 RCSB], [http://www.ebi.ac.uk/pdbsum/6gh7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gh7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6gh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gh7 OCA], [http://pdbe.org/6gh7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gh7 RCSB], [http://www.ebi.ac.uk/pdbsum/6gh7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gh7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).		[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	There has been growing interest in performing organocatalysis within a supramolecular system as a means of controlling reaction reactivity and stereoselectivity. Here, a protein is used as a host for iminium catalysis. A pyrrolidine moiety is covalently linked to biotin and introduced to the protein host streptavidin for organocatalytic activity. Whereas in traditional systems stereoselectivity is largely controlled by the substituents added to the organocatalyst, enantiomeric enrichment by the reported supramolecular system is completely controlled by the host. Also, the yield of the model reaction increases over 10-fold when streptavidin is included. A 1.1 A crystal structure of the protein-catalyst complex and molecular simulations of a key intermediate reveal the chiral scaffold surrounding the organocatalytic reaction site. This work illustrates that proteins can be an excellent supramolecular host for driving stereoselective secondary amine organocatalysis.
		+
		+	Reactivity and Selectivity of Iminium Organocatalysis Improved by a Protein Host.,Nodling AR, Swiderek K, Castillo R, Hall JW, Angelastro A, Morrill LC, Jin Y, Tsai YH, Moliner V, Luk LYP Angew Chem Int Ed Engl. 2018 Sep 17;57(38):12478-12482. doi:, 10.1002/anie.201806850. Epub 2018 Aug 23. PMID:30027571<ref>PMID:30027571</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6gh7" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Avidin 3D structures\|Avidin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: As 4 1583]]
		+	[[Category: Large Structures]]
	[[Category: Jin, Y]]		[[Category: Jin, Y]]
	[[Category: Luk, L Y.P]]		[[Category: Luk, L Y.P]]

OCA at 08:14, 10 October 2018

OCA — Wed, 10 Oct 2018 08:14:04 GMT

←Older revision		Revision as of 08:14, 10 October 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6gh7 ~~is ON HOLD until Paper Publication~~	+	==WILDTYPE CORE-STREPTAVIDIN WITH BIOTIN A with a catalyst==
-		+	<StructureSection load='6gh7' size='340' side='right' caption='[[6gh7]], [[Resolution\|resolution]] 1.08Å' scene=''>
-	~~Authors~~: ~~Nodling, A~~.R., ~~Tsai~~, Y.H., ~~Luk, L~~.Y.P., ~~Rizkallah~~, P., ~~Jin~~, Y.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6gh7]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GH7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GH7 FirstGlance]. <br>
-	~~Description~~: ~~WILDTYPE CORE~~-~~STREPTAVIDIN WITH BIOTIN A~~ with ~~a catalyst~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EYW:5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-[(3~{R})-pyrrolidin-3-yl]pentanamide'>EYW</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gh7 OCA], [http://pdbe.org/6gh7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gh7 RCSB], [http://www.ebi.ac.uk/pdbsum/6gh7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gh7 ProSAT]</span></td></tr>
-	[[Category: ~~Tsai~~, Y.H]]	+	</table>
-	[[Category: Nodling, A.R]]	+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Jin, Y]]
		+	[[Category: Luk, L Y.P]]
		+	[[Category: Nodling, A R]]
	[[Category: Rizkallah, P]]		[[Category: Rizkallah, P]]
-	[[Category: ~~Luk~~, L.Y.P]]	+	[[Category: Tsai, Y H]]
-	[[Category: ~~Jin, Y~~]]	+	[[Category: Artificial enzyme]]
		+	[[Category: Biotin-binding protein]]
		+	[[Category: Organocatalysis]]
		+	[[Category: Secondary amine]]
		+	[[Category: Supramolecular chemistry]]

OCA: Protected "6gh7" [edit=sysop:move=sysop]

OCA — Wed, 16 May 2018 04:13:13 GMT

Protected "6gh7" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:13, 16 May 2018

OCA: New page: '''Unreleased structure''' The entry 6gh7 is ON HOLD until Paper Publication Authors: Nodling, A.R., Tsai, Y.H., Luk, L.Y.P., Rizkallah, P., Jin, Y. Description: WILDTYPE CORE-STREPTAV...

OCA — Wed, 16 May 2018 04:13:12 GMT

New page: '''Unreleased structure''' The entry 6gh7 is ON HOLD until Paper Publication Authors: Nodling, A.R., Tsai, Y.H., Luk, L.Y.P., Rizkallah, P., Jin, Y. Description: WILDTYPE CORE-STREPTAV...

New page

'''Unreleased structure'''

The entry 6gh7 is ON HOLD until Paper Publication

Authors: Nodling, A.R., Tsai, Y.H., Luk, L.Y.P., Rizkallah, P., Jin, Y.

Description: WILDTYPE CORE-STREPTAVIDIN WITH BIOTIN A with a catalyst
[[Category: Unreleased Structures]]
[[Category: Tsai, Y.H]]
[[Category: Nodling, A.R]]
[[Category: Rizkallah, P]]
[[Category: Luk, L.Y.P]]
[[Category: Jin, Y]]