OCA at 08:19, 26 December 2018

OCA — Wed, 26 Dec 2018 08:19:32 GMT

←Older revision		Revision as of 08:19, 26 December 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6ghf is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of a GST variant==
		+	<StructureSection load='6ghf' size='340' side='right' caption='[[6ghf]], [[Resolution\|resolution]] 3.52Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6ghf]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GHF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GHF FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ghf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ghf OCA], [http://pdbe.org/6ghf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ghf RCSB], [http://www.ebi.ac.uk/pdbsum/6ghf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ghf ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Glutathione transferases (GSTs, EC. 2.5.1.18) are inducible multifunctional enzymes that are essential in the detoxification and degradation of toxic compounds. GSTs have considerable biotechnological potential. In the present work, a new method for the generation of synthetic GSTs was developed. Abiotic stress treatment of Phaseolus vulgaris and Glycine max plants led to the induction of total GST activity and allowed the creation of a GST-enriched cDNA library using degenerated GST-specific primers and reverse transcription-PCR. This library was further diversified by employing directed evolution through DNA shuffling. Activity screening of the evolved library led to the identification of a novel tau class GST enzyme (PvGmGSTUG). The enzyme was purified by affinity chromatography, characterized by kinetic analysis, and its structure was determined by X-ray crystallography. Interestingly, PvGmGSTUG displayed enhanced glutathione hydroperoxidase activity, which was significantly greater than that reported so far for natural tau class GSTs. In addition, the enzyme displayed unusual cooperative kinetics toward 1-chloro-2,4-dinitrochlorobenzene (CDNB) but not toward glutathione. The present work provides an easy approach for the simultaneous shuffling of GST genes from different plants, thus allowing the directed evolution of plants GSTome. This may permit the generation of new synthetic enzymes with interesting properties that are valuable in biotechnology.

-	~~Authors~~: ~~Papageorgiou, A.C~~., Chronopoulou, ~~E.G.~~, ~~Labrou~~, N.E.	+	Expanding the Plant GSTome Through Directed Evolution: DNA Shuffling for the Generation of New Synthetic Enzymes With Engineered Catalytic and Binding Properties.,Chronopoulou EG, Papageorgiou AC, Ataya F, Nianiou-Obeidat I, Madesis P, Labrou NE Front Plant Sci. 2018 Nov 30;9:1737. doi: 10.3389/fpls.2018.01737. eCollection, 2018. PMID:30555496<ref>PMID:30555496</ref>

-	~~Description: Crystal structure of~~ a ~~GST variant~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Papageorgiou, A.C]]	+	<div class="pdbe-citations 6ghf" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Chronopoulou, E.G~~]]	+	== References ==
-	[[Category: ~~Labrou, N.E~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chronopoulou, E G]]
		+	[[Category: Labrou, N E]]
		+	[[Category: Papageorgiou, A C]]
		+	[[Category: Biosynthetic protein]]
		+	[[Category: Gene shuffling]]
		+	[[Category: Transferase]]

OCA at 06:08, 23 May 2018

OCA — Wed, 23 May 2018 06:08:39 GMT

←Older revision		Revision as of 06:08, 23 May 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ghf is ON HOLD	+	The entry 6ghf is ON HOLD until Paper Publication

	Authors: Papageorgiou, A.C., Chronopoulou, E.G., Labrou, N.E.		Authors: Papageorgiou, A.C., Chronopoulou, E.G., Labrou, N.E.
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	Description: Crystal structure of a GST variant		Description: Crystal structure of a GST variant
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Papageorgiou, A.C]]
	[[Category: Chronopoulou, E.G]]		[[Category: Chronopoulou, E.G]]
	[[Category: Labrou, N.E]]		[[Category: Labrou, N.E]]
-	[[Category: Papageorgiou, A.C]]

OCA: Protected "6ghf" [edit=sysop:move=sysop]

OCA — Wed, 16 May 2018 04:14:10 GMT

Protected "6ghf" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:14, 16 May 2018

OCA: New page: '''Unreleased structure''' The entry 6ghf is ON HOLD Authors: Papageorgiou, A.C., Chronopoulou, E.G., Labrou, N.E. Description: Crystal structure of a GST variant [[Category: Unrelease...

OCA — Wed, 16 May 2018 04:14:09 GMT

New page: '''Unreleased structure''' The entry 6ghf is ON HOLD Authors: Papageorgiou, A.C., Chronopoulou, E.G., Labrou, N.E. Description: Crystal structure of a GST variant [[Category: Unrelease...

New page

'''Unreleased structure'''

The entry 6ghf is ON HOLD

Authors: Papageorgiou, A.C., Chronopoulou, E.G., Labrou, N.E.

Description: Crystal structure of a GST variant
[[Category: Unreleased Structures]]
[[Category: Chronopoulou, E.G]]
[[Category: Labrou, N.E]]
[[Category: Papageorgiou, A.C]]

6ghf - Revision history

OCA at 08:19, 26 December 2018

OCA at 06:08, 23 May 2018

OCA: Protected "6ghf" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6ghf is ON HOLD Authors: Papageorgiou, A.C., Chronopoulou, E.G., Labrou, N.E. Description: Crystal structure of a GST variant [[Category: Unrelease...