6ghq - Revision history

OCA at 08:33, 24 July 2019

OCA — Wed, 24 Jul 2019 08:33:52 GMT

←Older revision		Revision as of 08:33, 24 July 2019
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/HTXB_PSEST HTXB_PSEST]] Probably forms part of a binding-protein-dependent hypophosphite transporter.		[[http://www.uniprot.org/uniprot/HTXB_PSEST HTXB_PSEST]] Probably forms part of a binding-protein-dependent hypophosphite transporter.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Phosphorus acquisition is critical for life. In low phosphate conditions, some species of bacteria have evolved mechanisms to import reduced phosphorus compounds, such as phosphite and hypophosphite, as alternative phosphorus sources. Uptake is facilitated by high-affinity periplasmic binding proteins (PBPs) that bind cargo in the periplasm and shuttle it to an ATP-binding cassette (ABC)-transporter in the bacterial inner membrane. PtxB and HtxB are the PBPs responsible for binding phosphite and hypophosphite, respectively. They recognize the P-H bond of phosphite/hypophosphite via a conserved P-H...pi interaction, which confers nanomolar dissociation constants for their respective ligands. PtxB also has a low-level binding affinity for phosphate and hypophosphite, whilst HtxB can facilitate phosphite uptake in vivo. However, HtxB does not bind phosphate, thus the HtxBCDE transporter has recently been successfully exploited for biocontainment of genetically modified organisms by phosphite-dependent growth. Here we use a combination of X-ray crystallography, NMR and Microscale Thermophoresis to show that phosphite binding to HtxB depends on the protonation state of the ligand, suggesting that pH may effect the efficiency of phosphite uptake by HtxB in biotechnology applications.
		+
		+	Phosphite binding by the HtxB periplasmic binding protein depends on the protonation state of the ligand.,Adams NBP, Robertson AJ, Hunter CN, Hitchcock A, Bisson C Sci Rep. 2019 Jul 15;9(1):10231. doi: 10.1038/s41598-019-46557-2. PMID:31308436<ref>PMID:31308436</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6ghq" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:24, 26 June 2019

OCA — Wed, 26 Jun 2019 07:24:07 GMT

←Older revision		Revision as of 07:24, 26 June 2019
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	<StructureSection load='6ghq' size='340' side='right'caption='[[6ghq]], [[Resolution\|resolution]] 1.53Å' scene=''>		<StructureSection load='6ghq' size='340' side='right'caption='[[6ghq]], [[Resolution\|resolution]] 1.53Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ghq]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GHQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GHQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ghq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"achromobacter_sewerinii"_bergey_et_al._1923 "achromobacter sewerinii" bergey et al. 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GHQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GHQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6emn\|6emn]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6emn\|6emn]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">htxB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 "Achromobacter sewerinii" Bergey et al. 1923])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ghq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ghq OCA], [http://pdbe.org/6ghq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ghq RCSB], [http://www.ebi.ac.uk/pdbsum/6ghq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ghq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ghq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ghq OCA], [http://pdbe.org/6ghq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ghq RCSB], [http://www.ebi.ac.uk/pdbsum/6ghq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ghq ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Achromobacter sewerinii bergey et al. 1923]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Adams, N B]]		[[Category: Adams, N B]]

OCA at 06:46, 23 May 2019

OCA — Thu, 23 May 2019 06:46:27 GMT

←Older revision		Revision as of 06:46, 23 May 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6ghq is ~~ON HOLD~~	+	==HtxB D206N protein variant from Pseudomonas stutzeri in a partially open conformation to 1.53 A resolution==
-		+	<StructureSection load='6ghq' size='340' side='right'caption='[[6ghq]], [[Resolution\|resolution]] 1.53Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6ghq]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GHQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GHQ FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6emn\|6emn]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ghq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ghq OCA], [http://pdbe.org/6ghq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ghq RCSB], [http://www.ebi.ac.uk/pdbsum/6ghq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ghq ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HTXB_PSEST HTXB_PSEST]] Probably forms part of a binding-protein-dependent hypophosphite transporter.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Adams, N B]]
		+	[[Category: Bisson, C]]
		+	[[Category: Hitchcock, A]]
		+	[[Category: Robertson, A J]]
		+	[[Category: Abc-transporter]]
		+	[[Category: Hypophosphite]]
		+	[[Category: Periplasmic binding protein]]
		+	[[Category: Phosphite]]
		+	[[Category: Transport protein]]

OCA at 05:59, 30 January 2019

OCA — Wed, 30 Jan 2019 05:59:18 GMT

←Older revision		Revision as of 05:59, 30 January 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ghq is ON HOLD ~~until Paper Publication~~	+	The entry 6ghq is ON HOLD

	Authors:		Authors:

OCA: Protected "6ghq" [edit=sysop:move=sysop]

OCA — Wed, 16 May 2018 04:15:35 GMT

Protected "6ghq" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:15, 16 May 2018

OCA: New page: '''Unreleased structure''' The entry 6ghq is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 16 May 2018 04:15:34 GMT

New page: '''Unreleased structure''' The entry 6ghq is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6ghq is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]