6ghw - Revision history

OCA at 07:54, 24 April 2019

OCA — Wed, 24 Apr 2019 07:54:35 GMT

←Older revision		Revision as of 07:54, 24 April 2019
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	==Substituting the prolines of 4-oxalocrotonate tautomerase with non-canonical analogue (2S)-3,4-dehydroproline==		==Substituting the prolines of 4-oxalocrotonate tautomerase with non-canonical analogue (2S)-3,4-dehydroproline==
-	<StructureSection load='6ghw' size='340' side='right' caption='[[6ghw]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='6ghw' size='340' side='right'caption='[[6ghw]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ghw]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GHW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GHW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ghw]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GHW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GHW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=8LJ:'>8LJ</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=8LJ:'>8LJ</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">xylH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 "Bacillus fluorescens putidus" Flugge 1886])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-hydroxymuconate_tautomerase 2-hydroxymuconate tautomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.2.6 5.3.2.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-hydroxymuconate_tautomerase 2-hydroxymuconate tautomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.2.6 5.3.2.6] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ghw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ghw OCA], [http://pdbe.org/6ghw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ghw RCSB], [http://www.ebi.ac.uk/pdbsum/6ghw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ghw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ghw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ghw OCA], [http://pdbe.org/6ghw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ghw RCSB], [http://www.ebi.ac.uk/pdbsum/6ghw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ghw ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus fluorescens putidus flugge 1886]]
	[[Category: 2-hydroxymuconate tautomerase]]		[[Category: 2-hydroxymuconate tautomerase]]
		+	[[Category: Large Structures]]
	[[Category: Gruber, K]]		[[Category: Gruber, K]]
	[[Category: Lukesch, M S]]		[[Category: Lukesch, M S]]

OCA at 07:28, 6 March 2019

OCA — Wed, 06 Mar 2019 07:28:26 GMT

←Older revision		Revision as of 07:28, 6 March 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6ghw is ~~ON HOLD~~	+	==Substituting the prolines of 4-oxalocrotonate tautomerase with non-canonical analogue (2S)-3,4-dehydroproline==
		+	<StructureSection load='6ghw' size='340' side='right' caption='[[6ghw]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6ghw]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GHW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GHW FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=8LJ:'>8LJ</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-hydroxymuconate_tautomerase 2-hydroxymuconate tautomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.2.6 5.3.2.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ghw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ghw OCA], [http://pdbe.org/6ghw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ghw RCSB], [http://www.ebi.ac.uk/pdbsum/6ghw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ghw ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/4OT1_PSEPU 4OT1_PSEPU]] Catalyzes the ketonization of 2-hydroxymuconate stereoselectively to yield 2-oxo-3-hexenedioate.<ref>PMID:1339435</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The enzyme 4-oxalocrotonate tautomerase shows remarkable catalytic versatility due to the secondary amine of its N-terminal proline moiety. In this work, we incorporated a range of proline analogues into the enzyme and examined the effects on structure and activity. While the structure of the enzyme remained unperturbed, its promiscuous Michael-type activity was severely affected. This finding demonstrates how atomic changes in a biocatalytic system can abolish its activity. Our work provides a toolbox for successful generation of enzyme variants with non-canonical catalytic proline analogues.

-	~~Authors~~:	+	Substituting the catalytic proline of 4-oxalocrotonate tautomerase with non-canonical analogues reveals a finely tuned catalytic system.,Lukesch MS, Pavkov-Keller T, Gruber K, Zangger K, Wiltschi B Sci Rep. 2019 Feb 25;9(1):2697. doi: 10.1038/s41598-019-39484-9. PMID:30804446<ref>PMID:30804446</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6ghw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: 2-hydroxymuconate tautomerase]]
		+	[[Category: Gruber, K]]
		+	[[Category: Lukesch, M S]]
		+	[[Category: Pavkov-Keller, T]]
		+	[[Category: Wiltschi, B]]
		+	[[Category: 4-dehydroproline]]
		+	[[Category: Isomerase]]
		+	[[Category: Non-canonical amino acid]]

OCA: Protected "6ghw" [edit=sysop:move=sysop]

OCA — Wed, 16 May 2018 04:15:55 GMT

Protected "6ghw" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:15, 16 May 2018

OCA: New page: '''Unreleased structure''' The entry 6ghw is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 16 May 2018 04:15:54 GMT

New page: '''Unreleased structure''' The entry 6ghw is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6ghw is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]