6gid - Revision history

OCA at 07:55, 12 September 2018

OCA — Wed, 12 Sep 2018 07:55:03 GMT

←Older revision		Revision as of 07:55, 12 September 2018
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	<StructureSection load='6gid' size='340' side='right' caption='[[6gid]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='6gid' size='340' side='right' caption='[[6gid]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6gid]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GID OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GID FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6gid]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GID OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GID FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MME, EPN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Neprilysin Neprilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.11 3.4.24.11] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Neprilysin Neprilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.11 3.4.24.11] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gid FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gid OCA], [http://pdbe.org/6gid PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gid RCSB], [http://www.ebi.ac.uk/pdbsum/6gid PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gid ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gid FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gid OCA], [http://pdbe.org/6gid PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gid RCSB], [http://www.ebi.ac.uk/pdbsum/6gid PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gid ProSAT]</span></td></tr>
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	</div>		</div>
	<div class="pdbe-citations 6gid" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6gid" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Neprilysin\|Neprilysin]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Neprilysin]]		[[Category: Neprilysin]]
	[[Category: Acharya, K R]]		[[Category: Acharya, K R]]

OCA at 05:41, 27 June 2018

OCA — Wed, 27 Jun 2018 05:41:39 GMT

←Older revision		Revision as of 05:41, 27 June 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6gid is ~~ON HOLD~~	+	==High resolution crystal structure of substrate-free human neprilysin==
		+	<StructureSection load='6gid' size='340' side='right' caption='[[6gid]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6gid]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GID OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GID FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Neprilysin Neprilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.11 3.4.24.11] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gid FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gid OCA], [http://pdbe.org/6gid PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gid RCSB], [http://www.ebi.ac.uk/pdbsum/6gid PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gid ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NEP_HUMAN NEP_HUMAN]] Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.<ref>PMID:2531377</ref> <ref>PMID:15283675</ref> <ref>PMID:20876573</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Neprilysin is a transmembrane M13 zinc metalloprotease responsible for the degradation of several biologically active peptides including insulin, enkephalin, substance P, bradykinin, endothelin-1, neurotensin and amyloid-beta. The protein has received attention for its role in modulating blood pressure responses with its inhibition producing an antihypertensive response. To date, several inhibitor bound crystal structures of the human neprilysin extracellular domain have been determined, but, a structure free of bound inhibitor or substrate has yet to be reported. Here, we report the first crystal structure free of substrate or inhibitor for the extracellular catalytic domain of human neprilysin at 1.9A resolution. This structure will provide a reference point for comparisons to future inhibitor or substrate bound structures. The neprilysin structure also reveals that a closed protein conformation can be adopted in protein crystals absent of bound substrate or inhibitor.

-	~~Authors: Moss~~, S., Subramanian, V., Acharya, K.R.	+	High resolution crystal structure of substrate-free human neprilysin.,Moss S, Subramanian V, Acharya KR J Struct Biol. 2018 Jun 12. pii: S1047-8477(18)30142-4. doi:, 10.1016/j.jsb.2018.06.004. PMID:29906506<ref>PMID:29906506</ref>

-	~~Description: High resolution crystal structure~~ of ~~substrate~~-~~free human neprilysin~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Acharya, K.R]]	+	<div class="pdbe-citations 6gid" style="background-color:#fffaf0;"></div>
-	~~[[Category: Subramanian, V~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Neprilysin]]
		+	[[Category: Acharya, K R]]
	[[Category: Moss, S]]		[[Category: Moss, S]]
		+	[[Category: Subramanian, V]]
		+	[[Category: Hydrolase]]
		+	[[Category: Substrate-free]]

OCA: Protected "6gid" [edit=sysop:move=sysop]

OCA — Wed, 16 May 2018 04:16:59 GMT

Protected "6gid" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:16, 16 May 2018

OCA: New page: '''Unreleased structure''' The entry 6gid is ON HOLD Authors: Moss, S., Subramanian, V., Acharya, K.R. Description: High resolution crystal structure of substrate-free human neprilysin...

OCA — Wed, 16 May 2018 04:16:58 GMT

New page: '''Unreleased structure''' The entry 6gid is ON HOLD Authors: Moss, S., Subramanian, V., Acharya, K.R. Description: High resolution crystal structure of substrate-free human neprilysin...

New page

'''Unreleased structure'''

The entry 6gid is ON HOLD

Authors: Moss, S., Subramanian, V., Acharya, K.R.

Description: High resolution crystal structure of substrate-free human neprilysin
[[Category: Unreleased Structures]]
[[Category: Acharya, K.R]]
[[Category: Subramanian, V]]
[[Category: Moss, S]]