OCA at 07:46, 14 June 2018

OCA — Thu, 14 Jun 2018 07:46:06 GMT

←Older revision		Revision as of 07:46, 14 June 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6gk7 is ~~ON HOLD~~	+	==Crystal structure of anti-tau antibody dmCBTAU-27.1, double mutant (S31Y, T100I) of CBTAU-27.1, in complex with Tau peptide A8119B (residues 299-318)==
		+	<StructureSection load='6gk7' size='340' side='right' caption='[[6gk7]], [[Resolution\|resolution]] 2.95Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6gk7]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GK7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GK7 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6dcv\|6dcv]], [[6dcw\|6dcw]], [[5zv3\|5zv3]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gk7 OCA], [http://pdbe.org/6gk7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gk7 RCSB], [http://www.ebi.ac.uk/pdbsum/6gk7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gk7 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Misfolding and aggregation of tau protein are closely associated with the onset and progression of Alzheimer's Disease (AD). By interrogating IgG(+) memory B cells from asymptomatic donors with tau peptides, we have identified two somatically mutated VH5-51/VL4-1 antibodies. One of these, CBTAU-27.1, binds to the aggregation motif in the R3 repeat domain and blocks the aggregation of tau into paired helical filaments (PHFs) by sequestering monomeric tau. The other, CBTAU-28.1, binds to the N-terminal insert region and inhibits the spreading of tau seeds and mediates the uptake of tau aggregates into microglia by binding PHFs. Crystal structures revealed that the combination of VH5-51 and VL4-1 recognizes a common Pro-Xn-Lys motif driven by germline-encoded hotspot interactions while the specificity and thereby functionality of the antibodies are defined by the CDR3 regions. Affinity improvement led to improvement in functionality, identifying their epitopes as new targets for therapy and prevention of AD.

-	~~Authors: Steinbacher, S~~., Mrosek, M., ~~Juraszek~~, J.	+	A common antigenic motif recognized by naturally occurring human VH5-51/VL4-1 anti-tau antibodies with distinct functionalities.,Apetri A, Crespo R, Juraszek J, Pascual G, Janson R, Zhu X, Zhang H, Keogh E, Holland T, Wadia J, Verveen H, Siregar B, Mrosek M, Taggenbrock R, Ameijde J, Inganas H, van Winsen M, Koldijk MH, Zuijdgeest D, Borgers M, Dockx K, Stoop EJM, Yu W, Brinkman-van der Linden EC, Ummenthum K, van Kolen K, Mercken M, Steinbacher S, de Marco D, Hoozemans JJ, Wilson IA, Koudstaal W, Goudsmit J Acta Neuropathol Commun. 2018 May 31;6(1):43. doi: 10.1186/s40478-018-0543-z. PMID:29855358<ref>PMID:29855358</ref>

-	~~Description: Crystal structure~~ of ~~anti-tau antibody dmCBTAU-27~~.~~1, double mutant (S31Y, T100I)~~ of ~~CBTAU-27~~.~~1, in complex with Tau peptide A8119B (residues 299-318)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Steinbacher, S]]~~	+	<div class="pdbe-citations 6gk7" style="background-color:#fffaf0;"></div>
-	~~[[Category: Mrosek, M]]~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Juraszek, J]]		[[Category: Juraszek, J]]
		+	[[Category: Mrosek, M]]
		+	[[Category: Steinbacher, S]]
		+	[[Category: Antibody]]
		+	[[Category: Complex]]
		+	[[Category: Fab]]
		+	[[Category: Immune system]]
		+	[[Category: Tau]]

OCA: Protected "6gk7" [edit=sysop:move=sysop]

OCA — Wed, 23 May 2018 06:13:45 GMT

Protected "6gk7" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:13, 23 May 2018

OCA: New page: '''Unreleased structure''' The entry 6gk7 is ON HOLD Authors: Steinbacher, S., Mrosek, M., Juraszek, J. Description: Crystal structure of anti-tau antibody dmCBTAU-27.1, double mutant ...

OCA — Wed, 23 May 2018 06:13:44 GMT

New page: '''Unreleased structure''' The entry 6gk7 is ON HOLD Authors: Steinbacher, S., Mrosek, M., Juraszek, J. Description: Crystal structure of anti-tau antibody dmCBTAU-27.1, double mutant ...

New page

'''Unreleased structure'''

The entry 6gk7 is ON HOLD

Authors: Steinbacher, S., Mrosek, M., Juraszek, J.

Description: Crystal structure of anti-tau antibody dmCBTAU-27.1, double mutant (S31Y, T100I) of CBTAU-27.1, in complex with Tau peptide A8119B (residues 299-318)
[[Category: Unreleased Structures]]
[[Category: Steinbacher, S]]
[[Category: Mrosek, M]]
[[Category: Juraszek, J]]

6gk7 - Revision history

OCA at 07:46, 14 June 2018

OCA: Protected "6gk7" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6gk7 is ON HOLD Authors: Steinbacher, S., Mrosek, M., Juraszek, J. Description: Crystal structure of anti-tau antibody dmCBTAU-27.1, double mutant ...