OCA at 09:28, 30 January 2019

OCA — Wed, 30 Jan 2019 09:28:03 GMT

←Older revision		Revision as of 09:28, 30 January 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6gkb is ~~ON HOLD~~	+	==Iron soak structure of Y40F SynFtn==
		+	<StructureSection load='6gkb' size='340' side='right' caption='[[6gkb]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6gkb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Syns3 Syns3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GKB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GKB FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ouw\|5ouw]], [[5ouz\|5ouz]], [[6gkc\|6gkc]], [[6gka\|6gka]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sync_1539 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=64471 SYNS3])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bacterial_non-heme_ferritin Bacterial non-heme ferritin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.2 1.16.3.2] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gkb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gkb OCA], [http://pdbe.org/6gkb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gkb RCSB], [http://www.ebi.ac.uk/pdbsum/6gkb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gkb ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q0I9X8_SYNS3 Q0I9X8_SYNS3]] Iron-storage protein.[RuleBase:RU361145]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The gene encoding the cyanobacterial ferritin SynFtn is up-regulated in response to copper stress. Here, we show that, while SynFtn does not interact directly with copper, it is highly unusual in several ways. First, its catalytic diiron ferroxidase center is unlike those of all other characterized prokaryotic ferritins and instead resembles an animal H-chain ferritin center. Second, as demonstrated by kinetic, spectroscopic, and high-resolution X-ray crystallographic data, reaction of O2 with the di-Fe(2+) center results in a direct, one-electron oxidation to a mixed-valent Fe(2+)/Fe(3+) form. Iron-O2 chemistry of this type is currently unknown among the growing family of proteins that bind a diiron site within a four alpha-helical bundle in general and ferritins in particular. The mixed-valent form, which slowly oxidized to the more usual di-Fe(3+) form, is an intermediate that is continually generated during mineralization. Peroxide, rather than superoxide, is shown to be the product of O2 reduction, implying that ferroxidase centers function in pairs via long-range electron transfer through the protein resulting in reduction of O2 bound at only one of the centers. We show that electron transfer is mediated by the transient formation of a radical on Tyr40, which lies approximately 4 A from the diiron center. As well as demonstrating an expansion of the iron-O2 chemistry known to occur in nature, these data are also highly relevant to the question of whether all ferritins mineralize iron via a common mechanism, providing unequivocal proof that they do not.

-	~~Authors:~~ Hemmings, A.M., ~~Bradley, J~~.M.	+	Reaction of O2 with a diiron protein generates a mixed-valent Fe(2+)/Fe(3+) center and peroxide.,Bradley JM, Svistunenko DA, Pullin J, Hill N, Stuart RK, Palenik B, Wilson MT, Hemmings AM, Moore GR, Le Brun NE Proc Natl Acad Sci U S A. 2019 Jan 18. pii: 1809913116. doi:, 10.1073/pnas.1809913116. PMID:30659147<ref>PMID:30659147</ref>

-	~~Description: Iron soak structure~~ of ~~Y40F SynFtn~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Bradley, J.M]]	+	<div class="pdbe-citations 6gkb" style="background-color:#fffaf0;"></div>
-	[[Category: Hemmings, A.M]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bacterial non-heme ferritin]]
		+	[[Category: Syns3]]
		+	[[Category: Bradley, J M]]
		+	[[Category: Hemmings, A M]]
		+	[[Category: Ferritin]]
		+	[[Category: Metal binding protein]]

OCA: Protected "6gkb" [edit=sysop:move=sysop]

OCA — Wed, 30 May 2018 04:16:32 GMT

Protected "6gkb" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:16, 30 May 2018

OCA: New page: '''Unreleased structure''' The entry 6gkb is ON HOLD Authors: Hemmings, A.M., Bradley, J.M. Description: Iron soak structure of Y40F SynFtn Category: Unreleased Structures [[Catego...

OCA — Wed, 30 May 2018 04:16:31 GMT

New page: '''Unreleased structure''' The entry 6gkb is ON HOLD Authors: Hemmings, A.M., Bradley, J.M. Description: Iron soak structure of Y40F SynFtn Category: Unreleased Structures [[Catego...

New page

'''Unreleased structure'''

The entry 6gkb is ON HOLD

Authors: Hemmings, A.M., Bradley, J.M.

Description: Iron soak structure of Y40F SynFtn
[[Category: Unreleased Structures]]
[[Category: Bradley, J.M]]
[[Category: Hemmings, A.M]]

6gkb - Revision history

OCA at 09:28, 30 January 2019

OCA: Protected "6gkb" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6gkb is ON HOLD Authors: Hemmings, A.M., Bradley, J.M. Description: Iron soak structure of Y40F SynFtn Category: Unreleased Structures [[Catego...