6gm6 - Revision history

OCA at 07:50, 6 November 2019

OCA — Wed, 06 Nov 2019 07:50:43 GMT

←Older revision		Revision as of 07:50, 6 November 2019
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	==[FeFe]-hydrogenase HydA1 from Chlamydomonas reinhardtii,variant E141Q==		==[FeFe]-hydrogenase HydA1 from Chlamydomonas reinhardtii,variant E141Q==
-	<StructureSection load='6gm6' size='340' side='right' caption='[[6gm6]], [[Resolution\|resolution]] 1.61Å' scene=''>	+	<StructureSection load='6gm6' size='340' side='right'caption='[[6gm6]], [[Resolution\|resolution]] 1.61Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6gm6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GM6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GM6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6gm6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GM6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GM6 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3lx4\|3lx4]], [[4r0v\|4r0v]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hyd1, HYD1, hydA, hydA1, CHLRE_03g199800v5, CHLREDRAFT_183963 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3055 CHLRE])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferredoxin_hydrogenase Ferredoxin hydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.12.7.2 1.12.7.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferredoxin_hydrogenase Ferredoxin hydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.12.7.2 1.12.7.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gm6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gm6 OCA], [http://pdbe.org/6gm6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gm6 RCSB], [http://www.ebi.ac.uk/pdbsum/6gm6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gm6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gm6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gm6 OCA], [http://pdbe.org/6gm6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gm6 RCSB], [http://www.ebi.ac.uk/pdbsum/6gm6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gm6 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The unmatched catalytic turnover rates of [FeFe]-hydrogenases require an exceptionally efficient proton-transfer (PT) pathway to shuttle protons as substrates or products between bulk water and catalytic center. For clostridial [FeFe]-hydrogenase CpI such a pathway has been proposed and analyzed, but mainly on a theoretical basis. Here, eleven enzyme variants of two different [FeFe]-hydrogenases (CpI and HydA1) with substitutions in the presumptive PT-pathway are examined kinetically, spectroscopically, and crystallographically to provide solid experimental proof for its role in hydrogen-turnover. Targeting key residues of the PT-pathway by site directed mutagenesis significantly alters the pH-activity profile of these variants and in presence of H2 their cofactor is trapped in an intermediate state indicative of precluded proton-transfer. Furthermore, crystal structures coherently explain the individual levels of residual activity, demonstrating e.g. how trapped H2O molecules rescue the interrupted PT-pathway. These features provide conclusive evidence that the targeted positions are indeed vital for catalytic proton-transfer.
		+
		+	Crystallographic and spectroscopic assignment of the proton transfer pathway in [FeFe]-hydrogenases.,Duan J, Senger M, Esselborn J, Engelbrecht V, Wittkamp F, Apfel UP, Hofmann E, Stripp ST, Happe T, Winkler M Nat Commun. 2018 Nov 9;9(1):4726. doi: 10.1038/s41467-018-07140-x. PMID:30413719<ref>PMID:30413719</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6gm6" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chlre]]
	[[Category: Ferredoxin hydrogenase]]		[[Category: Ferredoxin hydrogenase]]
		+	[[Category: Large Structures]]
	[[Category: Duan, J]]		[[Category: Duan, J]]
	[[Category: Engelbrecht, V]]		[[Category: Engelbrecht, V]]

OCA at 12:19, 7 November 2018

OCA — Wed, 07 Nov 2018 12:19:55 GMT

←Older revision		Revision as of 12:19, 7 November 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6gm6 ~~is ON HOLD~~	+	==[FeFe]-hydrogenase HydA1 from Chlamydomonas reinhardtii,variant E141Q==
-		+	<StructureSection load='6gm6' size='340' side='right' caption='[[6gm6]], [[Resolution\|resolution]] 1.61Å' scene=''>
-	~~Authors~~: ~~Duan, J~~., ~~Engelbrecht~~, V., ~~Esselborn~~, J., ~~Hofmann~~, E., ~~Winkler, M~~., ~~Happe, T~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6gm6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GM6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GM6 FirstGlance]. <br>
-	~~Description: [FeFe]-hydrogenase HydA1 from Chlamydomonas reinhardtii,variant E141Q~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferredoxin_hydrogenase Ferredoxin hydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.12.7.2 1.12.7.2] </span></td></tr>
-	[[Category: ~~Winkler~~, M]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gm6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gm6 OCA], [http://pdbe.org/6gm6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gm6 RCSB], [http://www.ebi.ac.uk/pdbsum/6gm6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gm6 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Ferredoxin hydrogenase]]
		+	[[Category: Duan, J]]
	[[Category: Engelbrecht, V]]		[[Category: Engelbrecht, V]]
	[[Category: Esselborn, J]]		[[Category: Esselborn, J]]
-	[[Category: Duan, J]]
	[[Category: Happe, T]]		[[Category: Happe, T]]
	[[Category: Hofmann, E]]		[[Category: Hofmann, E]]
		+	[[Category: Winkler, M]]
		+	[[Category: Apo-form]]
		+	[[Category: H-cluster]]
		+	[[Category: Hydrogenase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Oxidoreductase]]

OCA: Protected "6gm6" [edit=sysop:move=sysop]

OCA — Wed, 30 May 2018 04:22:22 GMT

Protected "6gm6" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:22, 30 May 2018

OCA: New page: '''Unreleased structure''' The entry 6gm6 is ON HOLD Authors: Duan, J., Engelbrecht, V., Esselborn, J., Hofmann, E., Winkler, M., Happe, T. Description: [FeFe]-hydrogenase HydA1 from C...

OCA — Wed, 30 May 2018 04:22:21 GMT

New page: '''Unreleased structure''' The entry 6gm6 is ON HOLD Authors: Duan, J., Engelbrecht, V., Esselborn, J., Hofmann, E., Winkler, M., Happe, T. Description: [FeFe]-hydrogenase HydA1 from C...

New page

'''Unreleased structure'''

The entry 6gm6 is ON HOLD

Authors: Duan, J., Engelbrecht, V., Esselborn, J., Hofmann, E., Winkler, M., Happe, T.

Description: [FeFe]-hydrogenase HydA1 from Chlamydomonas reinhardtii,variant E141Q
[[Category: Unreleased Structures]]
[[Category: Winkler, M]]
[[Category: Engelbrecht, V]]
[[Category: Esselborn, J]]
[[Category: Duan, J]]
[[Category: Happe, T]]
[[Category: Hofmann, E]]