OCA at 07:18, 10 April 2019

OCA — Wed, 10 Apr 2019 07:18:49 GMT

←Older revision		Revision as of 07:18, 10 April 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6gmz is ~~ON HOLD~~	+	==Staphylopine dehydrogenase in complex with Histidine tag and citrate==
		+	<StructureSection load='6gmz' size='340' side='right'caption='[[6gmz]], [[Resolution\|resolution]] 2.22Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6gmz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GMZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GMZ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gmz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gmz OCA], [http://pdbe.org/6gmz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gmz RCSB], [http://www.ebi.ac.uk/pdbsum/6gmz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gmz ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Enzymatic regulations are central processes for the adaptation to changing environments. In the particular case of metallophore-dependent metal uptake, there is a need to quickly adjust the production of these metallophores to the metal level outside the cell, to avoid metal shortage or overload, as well as waste of metallophores. In Staphylococcus aureus, CntM catalyzes the last biosynthetic step in the production of staphylopine, a broad-spectrum metallophore, through the reductive condensation of a pathway intermediate (xNA) with pyruvate. Here, we describe the chemical synthesis of this intermediate, which was instrumental in the structural and functional characterization of CntM and confirmed its opine synthase properties. The three-dimensional structure of CntM was obtained in an "open" form, in the apo state or as a complex with substrate or product. The xNA substrate appears mainly stabilized by its imidazole ring through a pi-pi interaction with the side chain of Tyr240. Intriguingly, we found that metals exerted various and sometime antagonistic effects on the reaction catalyzed by CntM: zinc and copper are moderate activators at low concentration and then total inhibitors at higher concentration, whereas manganese is only an activator and cobalt and nickel are only inhibitors. We propose a model in which the relative affinity of a metal toward xNA and an inhibitory binding site on the enzyme controls activation, inhibition, or both as a function of metal concentration. This metal-dependent regulation of a metallophore-producing enzyme might also take place in vivo, which could contribute to the adjustment of metallophore production to the internal metal level.

-	~~Authors:~~ Hajjar, C., ~~Arnoux~~, P.	+	Control by Metals of Staphylopine Dehydrogenase Activity during Metallophore Biosynthesis.,Hajjar C, Fanelli R, Laffont C, Brutesco C, Cullia G, Tribout M, Nurizzo D, Borezee-Durant E, Voulhoux R, Pignol D, Lavergne J, Cavelier F, Arnoux P J Am Chem Soc. 2019 Apr 3;141(13):5555-5562. doi: 10.1021/jacs.9b01676. Epub 2019, Mar 22. PMID:30901200<ref>PMID:30901200</ref>

-	~~Description~~: ~~Staphylopine dehydrogenase in complex with Histidine tag and citrate~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6gmz" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Arnoux, P]]		[[Category: Arnoux, P]]
	[[Category: Hajjar, C]]		[[Category: Hajjar, C]]
		+	[[Category: Biosynthetic protein]]
		+	[[Category: Staphylopine biosynthesis]]

OCA: Protected "6gmz" [edit=sysop:move=sysop]

OCA — Wed, 06 Jun 2018 04:30:03 GMT

Protected "6gmz" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:30, 6 June 2018

OCA: New page: '''Unreleased structure''' The entry 6gmz is ON HOLD Authors: Hajjar, C., Arnoux, P. Description: Staphylopine dehydrogenase in complex with Histidine tag and citrate [[Category: Unrel...

OCA — Wed, 06 Jun 2018 04:30:01 GMT

New page: '''Unreleased structure''' The entry 6gmz is ON HOLD Authors: Hajjar, C., Arnoux, P. Description: Staphylopine dehydrogenase in complex with Histidine tag and citrate [[Category: Unrel...

New page

'''Unreleased structure'''

The entry 6gmz is ON HOLD

Authors: Hajjar, C., Arnoux, P.

Description: Staphylopine dehydrogenase in complex with Histidine tag and citrate
[[Category: Unreleased Structures]]
[[Category: Arnoux, P]]
[[Category: Hajjar, C]]

6gmz - Revision history

OCA at 07:18, 10 April 2019

OCA: Protected "6gmz" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6gmz is ON HOLD Authors: Hajjar, C., Arnoux, P. Description: Staphylopine dehydrogenase in complex with Histidine tag and citrate [[Category: Unrel...