OCA at 08:19, 26 December 2018

OCA — Wed, 26 Dec 2018 08:19:39 GMT

←Older revision		Revision as of 08:19, 26 December 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6gn6 is ~~ON HOLD until Paper~~ Publication	+	==Alpha-L-fucosidase isoenzyme 1 from Paenibacillus thiaminolyticus==
		+	<StructureSection load='6gn6' size='340' side='right' caption='[[6gn6]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6gn6]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GN6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GN6 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MAL:MALTOSE'>MAL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-L-fucosidase Alpha-L-fucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.51 3.2.1.51] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gn6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gn6 OCA], [http://pdbe.org/6gn6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gn6 RCSB], [http://www.ebi.ac.uk/pdbsum/6gn6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gn6 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	alpha-l-Fucosidase isoenzyme 1 from bacterium Paenibacillus thiaminolyticus is a member of the glycoside hydrolase family GH29 capable of cleaving l-fucose from nonreducing termini of oligosaccharides and glycoconjugates. Here we present the first crystal structure of this protein revealing a novel quaternary state within this family. The protein is in a unique hexameric assembly revealing the first observed case of active site complementation by a residue from an adjacent monomer in this family. Mutation of the complementing tryptophan residue caused changes in the catalytic properties including a shift of the pH optimum, a change of affinity to an artificial chromogenic substrate and a decreased reaction rate for a natural substrate. The wild-type enzyme was active on most of the tested naturally occurring oligosaccharides and capable of transglycosylation on a variety of acceptor molecules, including saccharides, alcohols or chromogenic substrates. Mutation of the complementing residue changed neither substrate specificity nor the preference for the type of transglycosylation acceptor molecule; however, the yields of the reactions were lower in both cases. Maltose molecules bound to the enzyme in the crystal structure identified surface carbohydrate-binding sites, possibly participating in binding of larger oligosaccharides.

-	~~Authors~~:	+	Active site complementation and hexameric arrangement in the GH family 29; a structure-function study of alpha-l-fucosidase isoenzyme 1 from Paenibacillus thiaminolyticus.,Kovalova T, Koval T, Benesova E, Vodickova P, Spiwok V, Lipovova P, Dohnalek J Glycobiology. 2019 Jan 1;29(1):59-73. doi: 10.1093/glycob/cwy078. PMID:30544181<ref>PMID:30544181</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6gn6" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Alpha-L-fucosidase]]
		+	[[Category: Dohnalek, J]]
		+	[[Category: Koval, T]]
		+	[[Category: Kovalova, T]]
		+	[[Category: Lipovova, P]]
		+	[[Category: Active site complementation]]
		+	[[Category: Alpha-l-fucosidase]]
		+	[[Category: Gh29]]
		+	[[Category: Hexamer]]
		+	[[Category: Hydrolase]]

OCA at 04:18, 20 June 2018

OCA — Wed, 20 Jun 2018 04:18:51 GMT

←Older revision		Revision as of 04:18, 20 June 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6gn6 is ON HOLD	+	The entry 6gn6 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6gn6" [edit=sysop:move=sysop]

OCA — Wed, 06 Jun 2018 04:30:58 GMT

Protected "6gn6" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:30, 6 June 2018

OCA: New page: '''Unreleased structure''' The entry 6gn6 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 06 Jun 2018 04:30:57 GMT

New page: '''Unreleased structure''' The entry 6gn6 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6gn6 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6gn6 - Revision history

OCA at 08:19, 26 December 2018

OCA at 04:18, 20 June 2018

OCA: Protected "6gn6" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6gn6 is ON HOLD Authors: Description: Category: Unreleased Structures