6grg - Revision history

OCA at 08:24, 6 March 2019

OCA — Wed, 06 Mar 2019 08:24:10 GMT

←Older revision		Revision as of 08:24, 6 March 2019
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	==E. coli Microcin synthetase McbBCD complex with pro-MccB17, ADP and phosphate bound==		==E. coli Microcin synthetase McbBCD complex with pro-MccB17, ADP and phosphate bound==
-	<StructureSection load='6grg' size='340' side='right' caption='[[6grg]], [[Resolution\|resolution]] 2.35Å' scene=''>	+	<StructureSection load='6grg' size='340' side='right' caption='[[6grg]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6grg]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GRG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GRG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6grg]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_mg1655 Escherichia coli mg1655]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GRG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GRG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=F6N:'>F6N</scene>, <scene name='pdbligand=F75:'>F75</scene>, <scene name='pdbligand=OTZ:'>OTZ</scene>, <scene name='pdbligand=TOZ:'>TOZ</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=F6N:'>F6N</scene>, <scene name='pdbligand=F75:'>F75</scene>, <scene name='pdbligand=OTZ:'>OTZ</scene>, <scene name='pdbligand=TOZ:'>TOZ</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mcbA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511145 Escherichia coli MG1655]), mcbB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511145 Escherichia coli MG1655]), mcbC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511145 Escherichia coli MG1655]), mcbD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511145 Escherichia coli MG1655])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6grg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6grg OCA], [http://pdbe.org/6grg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6grg RCSB], [http://www.ebi.ac.uk/pdbsum/6grg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6grg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6grg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6grg OCA], [http://pdbe.org/6grg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6grg RCSB], [http://www.ebi.ac.uk/pdbsum/6grg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6grg ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli mg1655]]
	[[Category: Ghilarov, D]]		[[Category: Ghilarov, D]]
	[[Category: Lawson, D M]]		[[Category: Lawson, D M]]

OCA at 08:25, 30 January 2019

OCA — Wed, 30 Jan 2019 08:25:47 GMT

←Older revision		Revision as of 08:25, 30 January 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6grg is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==E. coli Microcin synthetase McbBCD complex with pro-MccB17, ADP and phosphate bound==
		+	<StructureSection load='6grg' size='340' side='right' caption='[[6grg]], [[Resolution\|resolution]] 2.35Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6grg]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GRG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GRG FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=F6N:'>F6N</scene>, <scene name='pdbligand=F75:'>F75</scene>, <scene name='pdbligand=OTZ:'>OTZ</scene>, <scene name='pdbligand=TOZ:'>TOZ</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6grg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6grg OCA], [http://pdbe.org/6grg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6grg RCSB], [http://www.ebi.ac.uk/pdbsum/6grg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6grg ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MCBD_ECOLX MCBD_ECOLX]] Necessary to process the inactive microcin B17 (McbA) precursor into the active peptide. [[http://www.uniprot.org/uniprot/MCBA_ECOLX MCBA_ECOLX]] This glycine-rich peptide antibiotic inhibits DNA replication in many enteric bacteria, that leads to induction of the SOS repair system, massive DNA degradation and cell death. B17 inhibits type II topoisomerase by trapping an enzyme - DNA cleavable complex.<ref>PMID:1846808</ref> [[http://www.uniprot.org/uniprot/MCBC_ECOLX MCBC_ECOLX]] Necessary to process the inactive microcin B17 (McbA) precursor into the active peptide. [[http://www.uniprot.org/uniprot/MCBB_ECOLX MCBB_ECOLX]] Necessary to process the inactive microcin B17 (McbA) precursor into the active peptide.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The introduction of azole heterocycles into a peptide backbone is the principal step in the biosynthesis of numerous compounds with therapeutic potential. One of them is microcin B17, a bacterial topoisomerase inhibitor whose activity depends on the conversion of selected serine and cysteine residues of the precursor peptide to oxazoles and thiazoles by the McbBCD synthetase complex. Crystal structures of McbBCD reveal an octameric B4C2D2 complex with two bound substrate peptides. Each McbB dimer clamps the N-terminal recognition sequence, while the C-terminal heterocycle of the modified peptide is trapped in the active site of McbC. The McbD and McbC active sites are distant from each other, which necessitates alternate shuttling of the peptide substrate between them, while remaining tethered to the McbB dimer. An atomic-level view of the azole synthetase is a starting point for deeper understanding and control of biosynthesis of a large group of ribosomally synthesized natural products.

-	~~Authors~~:	+	Architecture of Microcin B17 Synthetase: An Octameric Protein Complex Converting a Ribosomally Synthesized Peptide into a DNA Gyrase Poison.,Ghilarov D, Stevenson CEM, Travin DY, Piskunova J, Serebryakova M, Maxwell A, Lawson DM, Severinov K Mol Cell. 2019 Jan 16. pii: S1097-2765(18)31002-5. doi:, 10.1016/j.molcel.2018.11.032. PMID:30661981<ref>PMID:30661981</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6grg" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Ghilarov, D]]
		+	[[Category: Lawson, D M]]
		+	[[Category: Maxwell, A]]
		+	[[Category: Piskunova, J]]
		+	[[Category: Serebryakova, M]]
		+	[[Category: Severinov, K]]
		+	[[Category: Stevenson, C E.M]]
		+	[[Category: Travin, D Y]]
		+	[[Category: Biosynthetic protein]]
		+	[[Category: Dna gyrase]]
		+	[[Category: Heterocyclization]]
		+	[[Category: Microcin]]
		+	[[Category: Posttranslational modification]]

OCA: Protected "6grg" [edit=sysop:move=sysop]

OCA — Wed, 20 Jun 2018 04:23:04 GMT

Protected "6grg" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:23, 20 June 2018

OCA: New page: '''Unreleased structure''' The entry 6grg is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 20 Jun 2018 04:23:02 GMT

New page: '''Unreleased structure''' The entry 6grg is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6grg is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]