6gsc - Revision history

OCA at 06:12, 12 June 2019

OCA — Wed, 12 Jun 2019 06:12:12 GMT

←Older revision		Revision as of 06:12, 12 June 2019
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	==Sphingobacterium sp. T2 manganese superoxide dismutase catalyses the oxidative demethylation of polymeric lignin via generation of hydroxyl radical==		==Sphingobacterium sp. T2 manganese superoxide dismutase catalyses the oxidative demethylation of polymeric lignin via generation of hydroxyl radical==
-	<StructureSection load='6gsc' size='340' side='right' caption='[[6gsc]], [[Resolution\|resolution]] 1.32Å' scene=''>	+	<StructureSection load='6gsc' size='340' side='right'caption='[[6gsc]], [[Resolution\|resolution]] 1.32Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6gsc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33861 Atcc 33861]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GSC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GSC FirstGlance]. <br>		<table><tr><td colspan='2'>[[6gsc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33861 Atcc 33861]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GSC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GSC FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Atcc 33861]]		[[Category: Atcc 33861]]
		+	[[Category: Large Structures]]
	[[Category: Superoxide dismutase]]		[[Category: Superoxide dismutase]]
-	[[Category: ~~BAUMBERGER~~, S]]	+	[[Category: Baumberger, S]]
-	[[Category: ~~BUGG~~, T D.H]]	+	[[Category: Bugg, T D.H]]
-	[[Category: ~~COTTYN~~, B]]	+	[[Category: Cottyn, B]]
	[[Category: Fulop, V]]		[[Category: Fulop, V]]
-	[[Category: ~~RASHID~~, G M.M]]	+	[[Category: Rashid, G M.M]]
	[[Category: Wilkinson, R C]]		[[Category: Wilkinson, R C]]
-	[[Category: ~~ZHANG~~, X]]	+	[[Category: Zhang, X]]
	[[Category: Lignin oxidation]]		[[Category: Lignin oxidation]]
	[[Category: Lignin valorisation]]		[[Category: Lignin valorisation]]

OCA at 07:19, 31 October 2018

OCA — Wed, 31 Oct 2018 07:19:58 GMT

←Older revision		Revision as of 07:19, 31 October 2018
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	<StructureSection load='6gsc' size='340' side='right' caption='[[6gsc]], [[Resolution\|resolution]] 1.32Å' scene=''>		<StructureSection load='6gsc' size='340' side='right' caption='[[6gsc]], [[Resolution\|resolution]] 1.32Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6gsc]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GSC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GSC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6gsc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33861 Atcc 33861]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GSC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GSC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1] </span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 33861]]
	[[Category: Superoxide dismutase]]		[[Category: Superoxide dismutase]]
	[[Category: BAUMBERGER, S]]		[[Category: BAUMBERGER, S]]

OCA at 07:54, 3 October 2018

OCA — Wed, 03 Oct 2018 07:54:03 GMT

←Older revision		Revision as of 07:54, 3 October 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6gsc is ~~ON HOLD until Paper~~ Publication	+	==Sphingobacterium sp. T2 manganese superoxide dismutase catalyses the oxidative demethylation of polymeric lignin via generation of hydroxyl radical==
		+	<StructureSection load='6gsc' size='340' side='right' caption='[[6gsc]], [[Resolution\|resolution]] 1.32Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6gsc]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GSC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GSC FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gsc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gsc OCA], [http://pdbe.org/6gsc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gsc RCSB], [http://www.ebi.ac.uk/pdbsum/6gsc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gsc ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/A0A0M3KL50_9SPHI A0A0M3KL50_9SPHI]] Destroys radicals which are normally produced within the cells and which are toxic to biological systems.[RuleBase:RU000414]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Sphingobacterium sp. T2 contains two extracellular manganese superoxide dismutase enzymes which exhibit unprecedented activity for lignin oxidation, but via an unknown mechanism. Enzymatic treatment of lignin model compounds gave products whose structures were indicative of aryl-C oxidative cleavage and demethylation, as well as alkene dihydroxylation and alcohol oxidation. 18O labelling studies on the SpMnSOD-catalysed oxidation of lignin model compound guiaiacylglycerol--guaiacyl ether indicated that the oxygen atom inserted by the enzyme is derived from superoxide or peroxide. Analysis of an alkali lignin treated by SpMnSOD1 by quantitative 31P NMR spectroscopy demonstrated 20-40% increases in phenolic and aliphatic OH content, consistent with lignin demethylation and some internal oxidative cleavage reactions. Assay for hydroxyl radical generation using a fluorometric hydroxyphenylfluorescein assay revealed the release of approximately 1 molar equivalent of hydroxyl radical by SpMnSOD1. Four amino acid replacements in SpMnSOD1 were investigated, and A31H or Y27H site-directed mutant enzymes were found to show no lignin demethylation activity according to 31P NMR analysis. Structure determination of the A31H and Y27H mutant enzymes reveals the repositioning of an N-terminal protein loop, leading to widening of a solvent channel at the dimer interface, which would provide increased solvent access to the Mn centre for hydroxyl radical generation.

-	~~Authors: RASHID, G.M~~.M., ~~ZHANG~~, X., Wilkinson~~, R.C.~~, Fulop, V~~., COTTYN~~, B~~., BAUMBERGER~~, S., ~~BUGG, T~~.D.H.	+	Sphingobacterium sp. T2 manganese superoxide dismutase catalyses the oxidative demethylation of polymeric lignin via generation of hydroxyl radical.,Rashid GMM, Zhang X, Wilkinson RC, Fulop V, Cottyn B, Baumberger S, Bugg TDH ACS Chem Biol. 2018 Sep 24. doi: 10.1021/acschembio.8b00557. PMID:30247873<ref>PMID:30247873</ref>

-	~~Description: Sphingobacterium sp. T2 manganese superoxide dismutase catalyses~~ the ~~oxidative demethylation~~ of ~~polymeric lignin via generation of hydroxyl radical~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Cottyn~~, B]]	+	<div class="pdbe-citations 6gsc" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Rashid~~, G.~~M.M~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Superoxide dismutase]]
		+	[[Category: BAUMBERGER, S]]
		+	[[Category: BUGG, T D.H]]
		+	[[Category: COTTYN, B]]
	[[Category: Fulop, V]]		[[Category: Fulop, V]]
-	[[Category: Wilkinson, R.C]]	+	[[Category: RASHID, G M.M]]
-	[[Category: ~~Bugg~~, ~~T.D.H~~]]	+	[[Category: Wilkinson, R C]]
-	[[Category: ~~Baumberger, S~~]]	+	[[Category: ZHANG, X]]
-	[[Category: ~~Zhang, X~~]]	+	[[Category: Lignin oxidation]]
		+	[[Category: Lignin valorisation]]
		+	[[Category: Manganese keywds 2 superoxide dismutase]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Sphingobacterium]]

OCA at 04:10, 27 June 2018

OCA — Wed, 27 Jun 2018 04:10:46 GMT

←Older revision		Revision as of 04:10, 27 June 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6gsc is ON HOLD	+	The entry 6gsc is ON HOLD until Paper Publication

-	Authors: ~~Fulop~~, V., Wilkinson, R.C., Fulop, V., ~~Wilkinson~~, R.C.	+	Authors: RASHID, G.M.M., ZHANG, X., Wilkinson, R.C., Fulop, V., COTTYN, B., BAUMBERGER, S., BUGG, T.D.H.

	Description: Sphingobacterium sp. T2 manganese superoxide dismutase catalyses the oxidative demethylation of polymeric lignin via generation of hydroxyl radical		Description: Sphingobacterium sp. T2 manganese superoxide dismutase catalyses the oxidative demethylation of polymeric lignin via generation of hydroxyl radical
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: ~~Wilkinson~~, R.C]]	+	[[Category: Cottyn, B]]
		+	[[Category: Rashid, G.M.M]]
	[[Category: Fulop, V]]		[[Category: Fulop, V]]
		+	[[Category: Wilkinson, R.C]]
		+	[[Category: Bugg, T.D.H]]
		+	[[Category: Baumberger, S]]
		+	[[Category: Zhang, X]]

OCA: Protected "6gsc" [edit=sysop:move=sysop]

OCA — Wed, 20 Jun 2018 04:25:44 GMT

Protected "6gsc" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:25, 20 June 2018

OCA: New page: '''Unreleased structure''' The entry 6gsc is ON HOLD Authors: Fulop, V., Wilkinson, R.C., Fulop, V., Wilkinson, R.C. Description: Sphingobacterium sp. T2 manganese superoxide dismutase...

OCA — Wed, 20 Jun 2018 04:25:43 GMT

New page: '''Unreleased structure''' The entry 6gsc is ON HOLD Authors: Fulop, V., Wilkinson, R.C., Fulop, V., Wilkinson, R.C. Description: Sphingobacterium sp. T2 manganese superoxide dismutase...

New page

'''Unreleased structure'''

The entry 6gsc is ON HOLD

Authors: Fulop, V., Wilkinson, R.C., Fulop, V., Wilkinson, R.C.

Description: Sphingobacterium sp. T2 manganese superoxide dismutase catalyses the oxidative demethylation of polymeric lignin via generation of hydroxyl radical
[[Category: Unreleased Structures]]
[[Category: Wilkinson, R.C]]
[[Category: Fulop, V]]