6gtx - Revision history

OCA at 09:12, 1 May 2019

OCA — Wed, 01 May 2019 09:12:25 GMT

←Older revision		Revision as of 09:12, 1 May 2019
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	==Crystal structure of the FimH lectin domain from E.coli K12 in complex with the dimannoside Man(alpha1-2)Man==		==Crystal structure of the FimH lectin domain from E.coli K12 in complex with the dimannoside Man(alpha1-2)Man==
-	<StructureSection load='6gtx' size='340' side='right' caption='[[6gtx]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='6gtx' size='340' side='right'caption='[[6gtx]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6gtx]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GTX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GTX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6gtx]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GTX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GTX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fimH, b4320, JW4283 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gtx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gtx OCA], [http://pdbe.org/6gtx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gtx RCSB], [http://www.ebi.ac.uk/pdbsum/6gtx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gtx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gtx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gtx OCA], [http://pdbe.org/6gtx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gtx RCSB], [http://www.ebi.ac.uk/pdbsum/6gtx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gtx ProSAT]</span></td></tr>
	</table>		</table>
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	</div>		</div>
	<div class="pdbe-citations 6gtx" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6gtx" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Adhesin 3D structures\|Adhesin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Canonica, F]]		[[Category: Canonica, F]]
	[[Category: Ernst, B]]		[[Category: Ernst, B]]

OCA at 12:08, 16 January 2019

OCA — Wed, 16 Jan 2019 12:08:29 GMT

←Older revision		Revision as of 12:08, 16 January 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6gtx is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of the FimH lectin domain from E.coli K12 in complex with the dimannoside Man(alpha1-2)Man==
		+	<StructureSection load='6gtx' size='340' side='right' caption='[[6gtx]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6gtx]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GTX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GTX FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gtx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gtx OCA], [http://pdbe.org/6gtx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gtx RCSB], [http://www.ebi.ac.uk/pdbsum/6gtx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gtx ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FIMH_ECOLI FIMH_ECOLI]] Involved in regulation of length and mediation of adhesion of type 1 fimbriae (but not necessary for the production of fimbriae). Adhesin responsible for the binding to D-mannose. It is laterally positioned at intervals in the structure of the type 1 fimbriae. In order to integrate FimH in the fimbriae FimF and FimG are needed.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Multivalent carbohydrate-lectin interactions at host-pathogen interfaces play a crucial role in the establishment of infections. Although competitive antagonists that prevent pathogen adhesion are promising anti-microbial drugs, the molecular mechanisms underlying these complex adhesion processes are still poorly understood. Here, we characterize the interactions between the fimbrial adhesin FimH from uropathogenic Escherichia coli strains and its natural high-mannose type N-glycan binding epitopes on uroepithelial glycoproteins. Crystal structures and a detailed kinetic characterization of ligand-binding and dissociation revealed that the binding pocket of FimH evolved such that it recognizes the terminal alpha(1-2)-, alpha(1-3)- and alpha(1-6)-linked mannosides of natural high-mannose type N-glycans with similar affinity. We demonstrate that the 2,000-fold higher affinity of the domain-separated state of FimH compared to its domain-associated state is ligand-independent and consistent with a thermodynamic cycle in which ligand-binding shifts the association equilibrium between the FimH lectin and the FimH pilin domain. Moreover, we show that a single N-glycan can bind up to three molecules of FimH, albeit with negative cooperativity, so that a molar excess of accessible N-glycans over FimH on the cell surface favors monovalent FimH binding. Our data provide pivotal insights into the adhesion properties of uropathogenic Escherichia coli strains to their target receptors and a solid basis for the development of effective FimH antagonists.

-	~~Authors~~:	+	Binding of the bacterial adhesin FimH to its natural, multivalent high-mannose type glycan targets.,Sauer MM, Jakob RP, Luber T, Canonica F, Navarra G, Ernst B, Unverzagt C, Maier T, Glockshuber R J Am Chem Soc. 2018 Dec 13. doi: 10.1021/jacs.8b10736. PMID:30543411<ref>PMID:30543411</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6gtx" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Canonica, F]]
		+	[[Category: Ernst, B]]
		+	[[Category: Glockshuber, R]]
		+	[[Category: Jakob, R P]]
		+	[[Category: Luber, T]]
		+	[[Category: Maier, T]]
		+	[[Category: Navarra, G]]
		+	[[Category: Sauer, M M]]
		+	[[Category: Unverzagt, C]]
		+	[[Category: Catch-bond]]
		+	[[Category: Cell adhesion]]
		+	[[Category: Infection]]
		+	[[Category: Lectin]]
		+	[[Category: Mannose]]
		+	[[Category: Type i pilus]]
		+	[[Category: Upec]]

OCA at 05:45, 18 July 2018

OCA — Wed, 18 Jul 2018 05:45:33 GMT

←Older revision		Revision as of 05:45, 18 July 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6gtx is ON HOLD	+	The entry 6gtx is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6gtx" [edit=sysop:move=sysop]

OCA — Wed, 27 Jun 2018 04:13:02 GMT

Protected "6gtx" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:13, 27 June 2018

OCA: New page: '''Unreleased structure''' The entry 6gtx is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Jun 2018 04:13:01 GMT

New page: '''Unreleased structure''' The entry 6gtx is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6gtx is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]