6gua - Revision history

OCA at 06:27, 19 June 2019

OCA — Wed, 19 Jun 2019 06:27:29 GMT

←Older revision		Revision as of 06:27, 19 June 2019
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	<StructureSection load='6gua' size='340' side='right'caption='[[6gua]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='6gua' size='340' side='right'caption='[[6gua]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6gua]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GUA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GUA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6gua]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Lacla Lacla]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GUA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GUA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LL1502, L138230 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272623 LACLA])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gua OCA], [http://pdbe.org/6gua PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gua RCSB], [http://www.ebi.ac.uk/pdbsum/6gua PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gua ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gua OCA], [http://pdbe.org/6gua PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gua RCSB], [http://www.ebi.ac.uk/pdbsum/6gua PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gua ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Lacla]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Scheidig, A J]]		[[Category: Scheidig, A J]]

OCA at 06:47, 23 May 2019

OCA — Thu, 23 May 2019 06:47:07 GMT

←Older revision		Revision as of 06:47, 23 May 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6gua is ~~ON HOLD until Paper~~ Publication	+	==Xylulose 5-phosphate phosphoketolase from Lactococcus lactis==
		+	<StructureSection load='6gua' size='340' side='right'caption='[[6gua]], [[Resolution\|resolution]] 1.95Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6gua]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GUA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GUA FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gua OCA], [http://pdbe.org/6gua PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gua RCSB], [http://www.ebi.ac.uk/pdbsum/6gua PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gua ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Phosphoketolases (PK) are TPP-dependent enzymes which play essential roles in carbohydrate metabolism of numerous bacteria. Depending on the substrate specificity PKs can be subdivided into xylulose 5-phosphate (X5P) specific PKs (XPKs) and PKs which accept both X5P and fructose 6-phosphate (F6P) (XFPKs). Despite their key metabolic importance, so far only the crystal structures of two XFPKs have been reported. There are no reported structures for any XPKs and for any complexes between PK and substrate. One of the major unknowns concerning PKs mechanism of action is related to the structural determinants of PKs substrate specificity for X5P or F6P. We report here the crystal structure of XPK from Lactococcus lactis (XPK-Ll) at 2.1A resolution. Using small angle X-ray scattering (SAXS) we proved that XPK-Ll is a dimer in solution. Towards better understanding of PKs substrate specificity, we performed flexible docking of TPP-X5P and TPP-F6P on crystal structures of XPK-Ll, two XFPKs and transketolase (TK). Calculated structure-based binding energies consistently support XPK-Ll preference for X5P. Analysis of structural models thus obtained show that substrates adopt moderately different conformation in PKs active sites following distinct networks of polar interactions. Based on the here reported structure of XPK-Ll we propose the most probable amino acid residues involved in the catalytic steps of reaction mechanism. Altogether our results suggest that PKs substrate preference for X5P or F6P is the outcome of a fine balance between specific binding network and dissimilar catalytic residues depending on the enzyme (XPK or XFPK) - substrate (X5P or F6P) couples.

-	~~Authors~~:	+	Crystal structure of a xylulose 5-phosphate phosphoketolase. Insights into the substrate specificity for xylulose 5-phosphate.,Scheidig AJ, Horvath D, Szedlacsek SE J Struct Biol. 2019 May 3. pii: S1047-8477(19)30093-0. doi:, 10.1016/j.jsb.2019.04.017. PMID:31059775<ref>PMID:31059775</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6gua" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Scheidig, A J]]
		+	[[Category: Szedlacsek, S E]]
		+	[[Category: Alpha-beta fold]]
		+	[[Category: Homodimer]]
		+	[[Category: Lyase]]
		+	[[Category: Thiamine diphosphate-dependent enzyme]]

OCA at 18:06, 19 September 2018

OCA — Wed, 19 Sep 2018 18:06:41 GMT

←Older revision		Revision as of 18:06, 19 September 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6gua is ON HOLD	+	The entry 6gua is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6gua" [edit=sysop:move=sysop]

OCA — Wed, 27 Jun 2018 04:14:06 GMT

Protected "6gua" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:14, 27 June 2018

OCA: New page: '''Unreleased structure''' The entry 6gua is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Jun 2018 04:14:05 GMT

New page: '''Unreleased structure''' The entry 6gua is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6gua is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]