6gv4 - Revision history

OCA at 10:08, 22 May 2024

OCA — Wed, 22 May 2024 10:08:27 GMT

←Older revision		Revision as of 10:08, 22 May 2024
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	<SX load='6gv4' size='340' side='right' viewer='molstar' caption='[[6gv4]], [[Resolution\|resolution]] 2.80Å' scene=''>		<SX load='6gv4' size='340' side='right' viewer='molstar' caption='[[6gv4]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6gv4]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~] and [~~http~~://en.wikipedia.org/wiki/Human_parechovirus_3 Human parechovirus 3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GV4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6GV4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6gv4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Human_parechovirus_3 Human parechovirus 3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GV4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GV4 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6gv4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gv4 OCA], [~~http~~://pdbe.org/6gv4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6gv4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6gv4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6gv4 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.8Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gv4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gv4 OCA], [https://pdbe.org/6gv4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gv4 RCSB], [https://www.ebi.ac.uk/pdbsum/6gv4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gv4 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q8BES5_9PICO Q8BES5_9PICO]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</SX>		</SX>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Human parechovirus 3]]		[[Category: Human parechovirus 3]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Butcher~~, S J~~]]	+	[[Category: Butcher SJ]]
-	[[Category: Domanska, A]]	+	[[Category: Domanska A]]
-	[[Category: Flatt~~, J W~~]]	+	[[Category: Flatt JW]]
-	[[Category: Geraets~~, J A~~]]	+	[[Category: Geraets JA]]
-	[[Category: Jukonen~~, J J.J]]~~	+	[[Category: Jukonen JJJ]]
-	~~[[Category: Antibody]]~~	+
-	~~[[Category: Human parechovirus]]~~	+
-	~~[[Category: Rna]]~~	+
-	~~[[Category: Virus~~]]	+

OCA at 11:19, 29 July 2020

OCA — Wed, 29 Jul 2020 11:19:33 GMT

←Older revision		Revision as of 11:19, 29 July 2020
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	==See Also==		==See Also==
	*[[Antibody 3D structures\|Antibody 3D structures]]		*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[3D structures of human antibody\|3D structures of human antibody]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 01:06, 11 April 2020

OCA — Sat, 11 Apr 2020 01:06:05 GMT

←Older revision		Revision as of 01:06, 11 April 2020
Line 3:		Line 3:
	<SX load='6gv4' size='340' side='right' viewer='molstar' caption='[[6gv4]], [[Resolution\|resolution]] 2.80Å' scene=''>		<SX load='6gv4' size='340' side='right' viewer='molstar' caption='[[6gv4]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6gv4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Human_parechovirus_3 Human parechovirus 3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GV4 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6GV4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6gv4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Human_parechovirus_3 Human parechovirus 3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GV4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6GV4 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6gv4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gv4 OCA], [http://pdbe.org/6gv4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gv4 RCSB], [http://www.ebi.ac.uk/pdbsum/6gv4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gv4 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6gv4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gv4 OCA], [http://pdbe.org/6gv4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gv4 RCSB], [http://www.ebi.ac.uk/pdbsum/6gv4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gv4 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 21:32, 6 March 2020

OCA — Fri, 06 Mar 2020 21:32:54 GMT

←Older revision		Revision as of 21:32, 6 March 2020
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	==High-resolution Cryo-EM of Fab-labeled human parechovirus 3==		==High-resolution Cryo-EM of Fab-labeled human parechovirus 3==
-	<~~StructureSection~~ load='6gv4' size='340' side='right'caption='[[6gv4]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<SX load='6gv4' size='340' side='right' viewer='molstar' caption='[[6gv4]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6gv4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Human_parechovirus_3 Human parechovirus 3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GV4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GV4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6gv4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Human_parechovirus_3 Human parechovirus 3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GV4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GV4 FirstGlance]. <br>
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	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Human]]		[[Category: Human]]
	[[Category: Human parechovirus 3]]		[[Category: Human parechovirus 3]]

OCA at 08:15, 3 April 2019

OCA — Wed, 03 Apr 2019 08:15:39 GMT

←Older revision		Revision as of 08:15, 3 April 2019
Line 1:		Line 1:

	==High-resolution Cryo-EM of Fab-labeled human parechovirus 3==		==High-resolution Cryo-EM of Fab-labeled human parechovirus 3==
-	<StructureSection load='6gv4' size='340' side='right' caption='[[6gv4]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='6gv4' size='340' side='right'caption='[[6gv4]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6gv4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Human_parechovirus_3 Human parechovirus 3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GV4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GV4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6gv4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Human_parechovirus_3 Human parechovirus 3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GV4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GV4 FirstGlance]. <br>
Line 15:		Line 15:
	</div>		</div>
	<div class="pdbe-citations 6gv4" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6gv4" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 21:		Line 24:
	[[Category: Human]]		[[Category: Human]]
	[[Category: Human parechovirus 3]]		[[Category: Human parechovirus 3]]
		+	[[Category: Large Structures]]
	[[Category: Butcher, S J]]		[[Category: Butcher, S J]]
	[[Category: Domanska, A]]		[[Category: Domanska, A]]

OCA at 07:06, 5 December 2018

OCA — Wed, 05 Dec 2018 07:06:45 GMT

←Older revision		Revision as of 07:06, 5 December 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6gv4 is ~~ON HOLD until Paper~~ Publication	+	==High-resolution Cryo-EM of Fab-labeled human parechovirus 3==
		+	<StructureSection load='6gv4' size='340' side='right' caption='[[6gv4]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6gv4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Human_parechovirus_3 Human parechovirus 3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GV4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GV4 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gv4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gv4 OCA], [http://pdbe.org/6gv4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gv4 RCSB], [http://www.ebi.ac.uk/pdbsum/6gv4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gv4 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Human parechovirus 3 (HPeV3) infection is associated with sepsis in neonates characterized by significant immune activation and subsequent tissue damage. Strategies to limit infection have been unsuccessful due to inadequate molecular diagnostic tools for early detection and lack of a vaccine or specific antiviral therapy. Towards the latter, we present a 2.8 A-resolution structure of HPeV3 in complex with fragments from a neutralizing human monoclonal antibody AT12-015 using cryo-EM and image reconstruction. Modeling revealed that the epitope extends across neighboring asymmetric units with contributions from capsid proteins VP0, VP1, and VP3. Antibody decoration was found to block binding of HPeV3 to cultured cells. Additionally at high-resolution, it was possible to model a stretch of RNA inside the virion and from this identify the key features that drive and stabilize protein-RNA association during assembly.IMPORTANCE HPeV3 is receiving increasing attention as a prevalent cause of sepsis-like symptoms in neonates, which despite the severity of disease, there are no effective treatments available. Structural and molecular insights into virus neutralization are urgently needed, especially as clinical cases are on the rise. Towards this goal, we present the first structure of HPeV3 in complex with fragments from a neutralizing monoclonal antibody. At high-resolution it was possible to precisely define the epitope that when targeted, prevents virions from binding to cells. Such an atomic-level description is useful for understanding host-pathogen interaction, viral pathogenesis mechanisms, and for finding potential cures for infection and disease.

-	~~Authors: Domanska,~~ A., Flatt~~, J.W.~~, Jukonen, J.J.J.~~, Geraets, J~~.A.~~, Butcher, S~~.J.	+	2.8 A resolution cryo-EM structure of human parechovirus 3 in complex with Fab from a neutralizing antibody.,Domanska A, Flatt JW, Jukonen JJJ, Geraets JA, Butcher SJ J Virol. 2018 Nov 21. pii: JVI.01597-18. doi: 10.1128/JVI.01597-18. PMID:30463974<ref>PMID:30463974</ref>

-	~~Description: High-resolution Cryo-EM~~ of ~~Fab~~-~~labeled human parechovirus 3~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Geraets~~, J.A]]	+	<div class="pdbe-citations 6gv4" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Human parechovirus 3]]
		+	[[Category: Butcher, S J]]
	[[Category: Domanska, A]]		[[Category: Domanska, A]]
-	[[Category: ~~Butcher~~, S.J]]	+	[[Category: Flatt, J W]]
-	[[Category: ~~Flatt~~, J.W]]	+	[[Category: Geraets, J A]]
-	[[Category: Jukonen, J.J.J]]	+	[[Category: Jukonen, J J.J]]
		+	[[Category: Antibody]]
		+	[[Category: Human parechovirus]]
		+	[[Category: Rna]]
		+	[[Category: Virus]]

OCA at 05:45, 18 July 2018

OCA — Wed, 18 Jul 2018 05:45:57 GMT

←Older revision		Revision as of 05:45, 18 July 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6gv4 is ON HOLD	+	The entry 6gv4 is ON HOLD until Paper Publication

	Authors: Domanska, A., Flatt, J.W., Jukonen, J.J.J., Geraets, J.A., Butcher, S.J.		Authors: Domanska, A., Flatt, J.W., Jukonen, J.J.J., Geraets, J.A., Butcher, S.J.
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	[[Category: Domanska, A]]		[[Category: Domanska, A]]
	[[Category: Butcher, S.J]]		[[Category: Butcher, S.J]]
-	[[Category: Jukonen, J.J.J]]
	[[Category: Flatt, J.W]]		[[Category: Flatt, J.W]]
		+	[[Category: Jukonen, J.J.J]]

OCA: Protected "6gv4" [edit=sysop:move=sysop]

OCA — Wed, 27 Jun 2018 04:16:19 GMT

Protected "6gv4" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:16, 27 June 2018

OCA: New page: '''Unreleased structure''' The entry 6gv4 is ON HOLD Authors: Domanska, A., Flatt, J.W., Jukonen, J.J.J., Geraets, J.A., Butcher, S.J. Description: High-resolution Cryo-EM of Fab-label...

OCA — Wed, 27 Jun 2018 04:16:18 GMT

New page: '''Unreleased structure''' The entry 6gv4 is ON HOLD Authors: Domanska, A., Flatt, J.W., Jukonen, J.J.J., Geraets, J.A., Butcher, S.J. Description: High-resolution Cryo-EM of Fab-label...

New page

'''Unreleased structure'''

The entry 6gv4 is ON HOLD

Authors: Domanska, A., Flatt, J.W., Jukonen, J.J.J., Geraets, J.A., Butcher, S.J.

Description: High-resolution Cryo-EM of Fab-labeled human parechovirus 3
[[Category: Unreleased Structures]]
[[Category: Geraets, J.A]]
[[Category: Domanska, A]]
[[Category: Butcher, S.J]]
[[Category: Jukonen, J.J.J]]
[[Category: Flatt, J.W]]