6gw0 - Revision history

OCA at 12:48, 7 November 2018

OCA — Wed, 07 Nov 2018 12:48:58 GMT

←Older revision		Revision as of 12:48, 7 November 2018
Line 3:		Line 3:
	<StructureSection load='6gw0' size='340' side='right' caption='[[6gw0]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='6gw0' size='340' side='right' caption='[[6gw0]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6gw0]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GW0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6gw0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Norovirus_hu/gii.1/7ek/hawaii/1971/usa Norovirus hu/gii.1/7ek/hawaii/1971/usa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GW0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TUD:TAUROCHENODEOXYCHOLIC+ACID'>TUD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TUD:TAUROCHENODEOXYCHOLIC+ACID'>TUD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6gvz\|6gvz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6gvz\|6gvz]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1208060 Norovirus Hu/GII.1/7EK/Hawaii/1971/USA])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gw0 OCA], [http://pdbe.org/6gw0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gw0 RCSB], [http://www.ebi.ac.uk/pdbsum/6gw0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gw0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gw0 OCA], [http://pdbe.org/6gw0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gw0 RCSB], [http://www.ebi.ac.uk/pdbsum/6gw0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gw0 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A recently developed human norovirus cell culture system revealed that the presence of bile enhanced or was an essential requirement for the growth of certain genotypes. Before this discovery, histo-blood group antigens (HBGAs) were the only well-studied co-factor known for human noroviruses and there was evidence that several genotypes poorly bound HBGAs. Therefore, the purpose of this study was to investigate how human norovirus capsid interact with bile acids. We found that bile acids had low micromolar affinities to GII.1, GII.10, and GII.19 capsids, but did not bind GI.1, GII.3, GII.4, or GII.17. We showed that bile acid bound at a partially conserved pocket on the norovirus capsid-protruding (P) domain using X-ray crystallography. An amino acid sequence alignment and structural analysis delivered an explanation of selective bile acid binding. Intriguingly, we discovered that binding of the bile acid was the critical step to stabilize several P domain loops that optimally placed an essential amino acid side chain (Asp375) to bind HBGAs in an otherwise HBGA non-binder (GII.1). Furthermore, bile acid enhanced HBGA binding for a known HBGA binder (GII.10). Altogether, these new data suggests that bile acid functions as a loop stabilizing regulator and enhancer of HBGA binding for certain norovirus genotypes.IMPORTANCE Given that human norovirus virions likely interact with bile acid during a natural infection and our evidence that an HBGA non-binder (GII.1) can be converted to an HBGA binder after bile acid binding is of major significance. Our data provides direct evidence that like HBGAs, bile acid interaction on the capsid is an important co-factor for certain genotypes. However, more unanswered questions seem to arise from these new discoveries. For example, is there an association between the bile acid requirement and prevalence of certain genotypes? That is, the GII.1 and GII.10 (bile acid binders) genotypes rarely caused outbreaks, whereas the GII.4 and GII.17 genotypes (bile acid non-binders) were responsible for large epidemics. Therefore, it seems plausible that certain genotypes require bile acids, whereas others have modified their bile acid requirements on the capsid.
		+
		+	Structural basis for human norovirus capsid binding to bile acids.,Kilic T, Koromyslova A, Hansman GS J Virol. 2018 Oct 24. pii: JVI.01581-18. doi: 10.1128/JVI.01581-18. PMID:30355683<ref>PMID:30355683</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6gw0" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Monoamine oxidase\|Monoamine oxidase]]		*[[Monoamine oxidase\|Monoamine oxidase]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Norovirus hu/gii 1/7ek/hawaii/1971/usa]]
	[[Category: Hansman, G S]]		[[Category: Hansman, G S]]
	[[Category: Kilic, T]]		[[Category: Kilic, T]]

OCA at 08:09, 17 October 2018

OCA — Wed, 17 Oct 2018 08:09:22 GMT

←Older revision		Revision as of 08:09, 17 October 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6gw0 is ~~ON HOLD until Paper Publication~~	+	==GII.1 human norovirus protruding domain in complex with taurochenodeoxycholate (TCDCA)==
		+	<StructureSection load='6gw0' size='340' side='right' caption='[[6gw0]], [[Resolution\|resolution]] 1.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6gw0]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GW0 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TUD:TAUROCHENODEOXYCHOLIC+ACID'>TUD</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6gvz\|6gvz]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gw0 OCA], [http://pdbe.org/6gw0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gw0 RCSB], [http://www.ebi.ac.uk/pdbsum/6gw0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gw0 ProSAT]</span></td></tr>
		+	</table>

-	~~Authors: Kilic, T., Hansman, G.S.~~	+	==See Also==
-		+	*[[Monoamine oxidase\|Monoamine oxidase]]
-	~~Description: GII.1 human norovirus protruding domain in complex with taurochenodeoxycholate (TCDCA)~~	+	__TOC__
-	[[~~Category: Unreleased Structures~~]]	+	</StructureSection>
-	[[Category: Hansman, G.S]]	+	[[Category: Hansman, G S]]
	[[Category: Kilic, T]]		[[Category: Kilic, T]]
		+	[[Category: Gii 1]]
		+	[[Category: Hbga]]
		+	[[Category: Norovirus]]
		+	[[Category: P domain]]
		+	[[Category: Tcdca]]
		+	[[Category: Viral protein]]

OCA at 20:01, 9 August 2018

OCA — Thu, 09 Aug 2018 20:01:50 GMT

←Older revision		Revision as of 20:01, 9 August 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6gw0 is ON HOLD	+	The entry 6gw0 is ON HOLD until Paper Publication

	Authors: Kilic, T., Hansman, G.S.		Authors: Kilic, T., Hansman, G.S.

-	Description: ~~Bile acid -HBGA interplay reveals a novel co-factor role of bile~~	+	Description: GII.1 human norovirus protruding domain in complex with taurochenodeoxycholate (TCDCA)
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Hansman, G.S]]		[[Category: Hansman, G.S]]
	[[Category: Kilic, T]]		[[Category: Kilic, T]]

OCA: Protected "6gw0" [edit=sysop:move=sysop]

OCA — Wed, 27 Jun 2018 04:20:00 GMT

Protected "6gw0" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:20, 27 June 2018

OCA: New page: '''Unreleased structure''' The entry 6gw0 is ON HOLD Authors: Kilic, T., Hansman, G.S. Description: Bile acid -HBGA interplay reveals a novel co-factor role of bile [[Category: Unrelea...

OCA — Wed, 27 Jun 2018 04:19:59 GMT

New page: '''Unreleased structure''' The entry 6gw0 is ON HOLD Authors: Kilic, T., Hansman, G.S. Description: Bile acid -HBGA interplay reveals a novel co-factor role of bile [[Category: Unrelea...

New page

'''Unreleased structure'''

The entry 6gw0 is ON HOLD

Authors: Kilic, T., Hansman, G.S.

Description: Bile acid -HBGA interplay reveals a novel co-factor role of bile
[[Category: Unreleased Structures]]
[[Category: Hansman, G.S]]
[[Category: Kilic, T]]