6gw6 - Revision history

OCA at 11:21, 26 August 2020

OCA — Wed, 26 Aug 2020 11:21:46 GMT

←Older revision		Revision as of 11:21, 26 August 2020
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	==Structure of the Pseudomonas putida RES-Xre toxin-antitoxin complex==		==Structure of the Pseudomonas putida RES-Xre toxin-antitoxin complex==
-	<StructureSection load='6gw6' size='340' side='right' caption='[[6gw6]], [[Resolution\|resolution]] 2.21Å' scene=''>	+	<StructureSection load='6gw6' size='340' side='right'caption='[[6gw6]], [[Resolution\|resolution]] 2.21Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6gw6]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GW6 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca.weizmann.ac~~.~~il/oca-docs~~/fgij/fg.htm?mol=6GW6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6gw6]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Psepk Psepk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GW6 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6GW6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6gw6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gw6 OCA], [http://pdbe.org/6gw6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gw6 RCSB], [http://www.ebi.ac.uk/pdbsum/6gw6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gw6 ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AYO08_12385, B7H19_10550 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160488 PSEPK]), AYO08_12380, B7H19_10555, CBL13_00496, CBP06_13110 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160488 PSEPK])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6gw6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gw6 OCA], [http://pdbe.org/6gw6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gw6 RCSB], [http://www.ebi.ac.uk/pdbsum/6gw6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gw6 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Psepk]]
	[[Category: Brodersen, D E]]		[[Category: Brodersen, D E]]
	[[Category: Senissar, M]]		[[Category: Senissar, M]]

OCA at 05:58, 24 October 2018

OCA — Wed, 24 Oct 2018 05:58:30 GMT

←Older revision		Revision as of 05:58, 24 October 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6gw6 is ~~ON HOLD until Paper~~ Publication	+	==Structure of the Pseudomonas putida RES-Xre toxin-antitoxin complex==
		+	<StructureSection load='6gw6' size='340' side='right' caption='[[6gw6]], [[Resolution\|resolution]] 2.21Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6gw6]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GW6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GW6 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gw6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gw6 OCA], [http://pdbe.org/6gw6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gw6 RCSB], [http://www.ebi.ac.uk/pdbsum/6gw6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gw6 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Type II toxin-antitoxin (TA) modules, which are important cellular regulators in prokaryotes, usually encode two proteins, a toxin that inhibits cell growth and a non-toxic and labile inhibitor (antitoxin) that binds to and neutralizes the toxin. Here, we demonstrate that the res-xre locus from Photorhabdus luminescens and other bacterial species function as bona fide TA modules in E. coli. The 2.2 A crystal structure of the intact Pseudomonas putida RES-Xre TA complex reveals an unusual 2:4 stoichiometry in which a central RES toxin dimer binds two Xre antitoxin dimers. The antitoxin dimers each expose two helix-turn-helix (HTH) DNA binding domains of the Cro repressor type, suggesting the TA complex is capable of binding the upstream promoter sequence on DNA. The toxin core domain shows structural similarity to ADP-ribosylating enzymes such as diphtheria toxin but has an atypical NAD(+) -binding pocket suggesting an alternative function. We show that activation of the toxin in vivo causes a depletion of intracellular NAD(+) levels eventually leading to inhibition of cell growth in E. coli and inhibition of global macromolecular biosynthesis. Both structure and activity are unprecedented among bacterial toxin-antitoxin systems, suggesting the functional scope of bacterial TA toxins is much wider than previously appreciated. This article is protected by copyright. All rights reserved.

-	~~Authors~~:	+	The RES domain toxins of RES-Xre toxin-antitoxin modules induce cell stasis by degrading NAD().,Skjerning RB, Senissar M, Winther K, Gerdes K, Brodersen DE Mol Microbiol. 2018 Oct 13. doi: 10.1111/mmi.14150. PMID:30315706<ref>PMID:30315706</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6gw6" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Brodersen, D E]]
		+	[[Category: Senissar, M]]
		+	[[Category: Cro repressor]]
		+	[[Category: Nad+ glycohydrolase]]
		+	[[Category: Nadase]]
		+	[[Category: Res domain]]
		+	[[Category: Toxin]]
		+	[[Category: Toxin antitoxin type ii system]]
		+	[[Category: Xre]]

OCA at 05:52, 4 July 2018

OCA — Wed, 04 Jul 2018 05:52:36 GMT

←Older revision		Revision as of 05:52, 4 July 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6gw6 is ON HOLD	+	The entry 6gw6 is ON HOLD until Paper Publication

-	Authors: ~~Senissar, M., Brodersen, D.E.~~	+	Authors:

-	Description: ~~X-ray structure of the new family Toxin-Antitoxin type II system~~	+	Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Brodersen, D.E]]
-	[[Category: Senissar, M]]

OCA: Protected "6gw6" [edit=sysop:move=sysop]

OCA — Wed, 27 Jun 2018 04:20:21 GMT

Protected "6gw6" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:20, 27 June 2018

OCA: New page: '''Unreleased structure''' The entry 6gw6 is ON HOLD Authors: Senissar, M., Brodersen, D.E. Description: X-ray structure of the new family Toxin-Antitoxin type II system [[Category: Un...

OCA — Wed, 27 Jun 2018 04:20:20 GMT

New page: '''Unreleased structure''' The entry 6gw6 is ON HOLD Authors: Senissar, M., Brodersen, D.E. Description: X-ray structure of the new family Toxin-Antitoxin type II system [[Category: Un...

New page

'''Unreleased structure'''

The entry 6gw6 is ON HOLD

Authors: Senissar, M., Brodersen, D.E.

Description: X-ray structure of the new family Toxin-Antitoxin type II system
[[Category: Unreleased Structures]]
[[Category: Brodersen, D.E]]
[[Category: Senissar, M]]