6h07 - Revision history

OCA at 07:52, 6 November 2019

OCA — Wed, 06 Nov 2019 07:52:17 GMT

←Older revision		Revision as of 07:52, 6 November 2019
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	==X-ray structure of Lactobacillus brevis alcohol dehydrogenase==		==X-ray structure of Lactobacillus brevis alcohol dehydrogenase==
-	<StructureSection load='6h07' size='340' side='right' caption='[[6h07]], [[Resolution\|resolution]] 1.48Å' scene=''>	+	<StructureSection load='6h07' size='340' side='right'caption='[[6h07]], [[Resolution\|resolution]] 1.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6h07]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H07 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H07 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6h07]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_casei_g"_von_freudenreich_and_thoni_1904 "bacillus casei g" von freudenreich and thoni 1904]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H07 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H07 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[~~h61m~~\|~~h61m~~]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6h1m\|6h1m]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">radh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1580 "Bacillus casei g" von Freudenreich and Thoni 1904])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h07 OCA], [http://pdbe.org/6h07 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h07 RCSB], [http://www.ebi.ac.uk/pdbsum/6h07 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h07 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h07 OCA], [http://pdbe.org/6h07 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h07 RCSB], [http://www.ebi.ac.uk/pdbsum/6h07 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h07 ProSAT]</span></td></tr>
	</table>		</table>
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	</div>		</div>
	<div class="pdbe-citations 6h07" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6h07" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Alcohol dehydrogenase 3D structures\|Alcohol dehydrogenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus casei g von freudenreich and thoni 1904]]
		+	[[Category: Large Structures]]
	[[Category: Biggel, P]]		[[Category: Biggel, P]]
	[[Category: Hekmat, D]]		[[Category: Hekmat, D]]

OCA at 06:50, 12 December 2018

OCA — Wed, 12 Dec 2018 06:50:27 GMT

←Older revision		Revision as of 06:50, 12 December 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6h07 is ~~ON HOLD until Paper~~ Publication	+	==X-ray structure of Lactobacillus brevis alcohol dehydrogenase==
		+	<StructureSection load='6h07' size='340' side='right' caption='[[6h07]], [[Resolution\|resolution]] 1.48Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6h07]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H07 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H07 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[h61m\|h61m]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h07 OCA], [http://pdbe.org/6h07 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h07 RCSB], [http://www.ebi.ac.uk/pdbsum/6h07 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h07 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lactobacillus brevis alcohol dehydrogenase (LbADH) is a well studied homotetrameric enzyme which catalyzes the enantioselective reduction of prochiral ketones to the corresponding secondary alcohols. LbADH is stable and enzymatically active at elevated temperatures and accepts a broad range of substrates, making it a valuable tool in industrial biocatalysis. Here, the expression, purification and crystallization of LbADH to generate large, single crystals with a volume of up to 1 mm(3) suitable for neutron diffraction studies are described. Neutron diffraction data were collected from an H/D-exchanged LbADH crystal using the BIODIFF instrument at the Heinz Maier-Leibnitz Zentrum (MLZ), Garching, Germany to a resolution dmin of 2.15 A in 16 days. This allowed the first neutron crystal structure of LbADH to be determined. The neutron structure revealed new details of the hydrogen-bonding network originating from the ion-binding site of LbADH and provided new insights into the reasons why divalent magnesium (Mg(2+)) or manganese (Mn(2+)) ions are necessary for its activity. X-ray diffraction data were obtained from the same crystal at the European Synchrotron Radiation Facility (ESRF), Grenoble, France to a resolution dmin of 1.48 A. The high-resolution X-ray structure suggested partial occupancy of Mn(2+) and Mg(2+) at the ion-binding site. This is supported by the different binding affinity of Mn(2+) and Mg(2+) to the tetrameric structure calculated via free-energy molecular-dynamics simulations.

-	~~Authors~~:	+	Neutron and X-ray crystal structures of Lactobacillus brevis alcohol dehydrogenase reveal new insights into hydrogen-bonding pathways.,Hermann J, Nowotny P, Schrader TE, Biggel P, Hekmat D, Weuster-Botz D Acta Crystallogr F Struct Biol Commun. 2018 Dec 1;74(Pt 12):754-764. doi:, 10.1107/S2053230X18015273. Epub 2018 Nov 26. PMID:30511668<ref>PMID:30511668</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6h07" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Biggel, P]]
		+	[[Category: Hekmat, D]]
		+	[[Category: Hermann, J]]
		+	[[Category: Nowotny, P]]
		+	[[Category: Schneider, S]]
		+	[[Category: Weuster-Botz, D]]
		+	[[Category: Magnesium dependence]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: R-specific alcohol dehydrogenase]]
		+	[[Category: Short chain reductases/dehydrogenase]]

OCA at 14:58, 15 August 2018

OCA — Wed, 15 Aug 2018 14:58:33 GMT

←Older revision		Revision as of 14:58, 15 August 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6h07 is ON HOLD	+	The entry 6h07 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6h07" [edit=sysop:move=sysop]

OCA — Wed, 18 Jul 2018 05:46:57 GMT

Protected "6h07" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:46, 18 July 2018

OCA: New page: '''Unreleased structure''' The entry 6h07 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 18 Jul 2018 05:46:55 GMT

New page: '''Unreleased structure''' The entry 6h07 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6h07 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]