6h1m - Revision history

OCA at 11:32, 24 January 2024

OCA — Wed, 24 Jan 2024 11:32:13 GMT

←Older revision		Revision as of 11:32, 24 January 2024
Line 1:		Line 1:

	==Neutron structure of Lactobacillus brevis alcohol dehydrogenase==		==Neutron structure of Lactobacillus brevis alcohol dehydrogenase==
-	<StructureSection load='6h1m' size='340' side='right' caption='[[6h1m]], [[Resolution\|resolution]] 2.15Å' scene=''>	+	<StructureSection load='6h1m' size='340' side='right'caption='[[6h1m]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6h1m]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H1M OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6H1M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6h1m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Levilactobacillus_brevis Levilactobacillus brevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H1M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6H1M FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=MG:MAGNESIUM+ION'>MG</scene~~>, ~~<scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Neutron Diffraction, [[Resolution\|Resolution]] 2.15Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[6h07\|6h07]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6h1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h1m OCA], [~~http~~://pdbe.org/6h1m PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6h1m RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6h1m PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6h1m ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6h1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h1m OCA], [https://pdbe.org/6h1m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6h1m RCSB], [https://www.ebi.ac.uk/pdbsum/6h1m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6h1m ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q84EX5_LEVBR Q84EX5_LEVBR]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 6h1m" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6h1m" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Alcohol dehydrogenase 3D structures\|Alcohol dehydrogenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Biggel, P~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Hekmat, D~~]]	+	[[Category: Levilactobacillus brevis]]
-	[[Category: ~~Hermann, J]]~~	+	[[Category: Biggel P]]
-	~~[[Category: Nowotny,~~ P]]	+	[[Category: Hekmat D]]
-	[[Category: ~~Schrader, T E~~]]	+	[[Category: Hermann J]]
-	[[Category: ~~Weuster-Botz, D~~]]	+	[[Category: Nowotny P]]
-	[[Category: ~~Magnesium dependence~~]]	+	[[Category: Schrader TE]]
-	[[Category: ~~Oxidoreductase~~]]	+	[[Category: Weuster-Botz D]]
-	[[Category: R-~~specific alcohol dehydrogenase]]~~	+
-	~~[[Category: Short chain reductases/dehydrogenase~~]]	+

OCA at 06:50, 12 December 2018

OCA — Wed, 12 Dec 2018 06:50:28 GMT

←Older revision		Revision as of 06:50, 12 December 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6h1m is ~~ON HOLD~~	+	==Neutron structure of Lactobacillus brevis alcohol dehydrogenase==
		+	<StructureSection load='6h1m' size='340' side='right' caption='[[6h1m]], [[Resolution\|resolution]] 2.15Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6h1m]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H1M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H1M FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6h07\|6h07]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h1m OCA], [http://pdbe.org/6h1m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h1m RCSB], [http://www.ebi.ac.uk/pdbsum/6h1m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h1m ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lactobacillus brevis alcohol dehydrogenase (LbADH) is a well studied homotetrameric enzyme which catalyzes the enantioselective reduction of prochiral ketones to the corresponding secondary alcohols. LbADH is stable and enzymatically active at elevated temperatures and accepts a broad range of substrates, making it a valuable tool in industrial biocatalysis. Here, the expression, purification and crystallization of LbADH to generate large, single crystals with a volume of up to 1 mm(3) suitable for neutron diffraction studies are described. Neutron diffraction data were collected from an H/D-exchanged LbADH crystal using the BIODIFF instrument at the Heinz Maier-Leibnitz Zentrum (MLZ), Garching, Germany to a resolution dmin of 2.15 A in 16 days. This allowed the first neutron crystal structure of LbADH to be determined. The neutron structure revealed new details of the hydrogen-bonding network originating from the ion-binding site of LbADH and provided new insights into the reasons why divalent magnesium (Mg(2+)) or manganese (Mn(2+)) ions are necessary for its activity. X-ray diffraction data were obtained from the same crystal at the European Synchrotron Radiation Facility (ESRF), Grenoble, France to a resolution dmin of 1.48 A. The high-resolution X-ray structure suggested partial occupancy of Mn(2+) and Mg(2+) at the ion-binding site. This is supported by the different binding affinity of Mn(2+) and Mg(2+) to the tetrameric structure calculated via free-energy molecular-dynamics simulations.

-	~~Authors: Hermann~~, J., Nowotny, P., Schrader~~, T.E.~~, Biggel, P., Hekmat, D., Weuster-Botz, D.	+	Neutron and X-ray crystal structures of Lactobacillus brevis alcohol dehydrogenase reveal new insights into hydrogen-bonding pathways.,Hermann J, Nowotny P, Schrader TE, Biggel P, Hekmat D, Weuster-Botz D Acta Crystallogr F Struct Biol Commun. 2018 Dec 1;74(Pt 12):754-764. doi:, 10.1107/S2053230X18015273. Epub 2018 Nov 26. PMID:30511668<ref>PMID:30511668</ref>

-	~~Description: Neutron structure~~ of ~~Lactobacillus brevis alcohol dehydrogenase~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Hermann~~, J]]	+	<div class="pdbe-citations 6h1m" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Biggel, P]]
	[[Category: Hekmat, D]]		[[Category: Hekmat, D]]
-	[[Category: ~~Schrader~~, ~~T.E~~]]	+	[[Category: Hermann, J]]
	[[Category: Nowotny, P]]		[[Category: Nowotny, P]]
		+	[[Category: Schrader, T E]]
	[[Category: Weuster-Botz, D]]		[[Category: Weuster-Botz, D]]
-	[[Category: ~~Biggel, P~~]]	+	[[Category: Magnesium dependence]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: R-specific alcohol dehydrogenase]]
		+	[[Category: Short chain reductases/dehydrogenase]]

OCA: Protected "6h1m" [edit=sysop:move=sysop]

OCA — Wed, 18 Jul 2018 05:54:55 GMT

Protected "6h1m" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:54, 18 July 2018

OCA: New page: '''Unreleased structure''' The entry 6h1m is ON HOLD Authors: Hermann, J., Nowotny, P., Schrader, T.E., Biggel, P., Hekmat, D., Weuster-Botz, D. Description: Neutron structure of Lacto...

OCA — Wed, 18 Jul 2018 05:54:53 GMT

New page: '''Unreleased structure''' The entry 6h1m is ON HOLD Authors: Hermann, J., Nowotny, P., Schrader, T.E., Biggel, P., Hekmat, D., Weuster-Botz, D. Description: Neutron structure of Lacto...

New page

'''Unreleased structure'''

The entry 6h1m is ON HOLD

Authors: Hermann, J., Nowotny, P., Schrader, T.E., Biggel, P., Hekmat, D., Weuster-Botz, D.

Description: Neutron structure of Lactobacillus brevis alcohol dehydrogenase
[[Category: Unreleased Structures]]
[[Category: Hermann, J]]
[[Category: Hekmat, D]]
[[Category: Schrader, T.E]]
[[Category: Nowotny, P]]
[[Category: Weuster-Botz, D]]
[[Category: Biggel, P]]