OCA at 08:06, 29 August 2018

OCA — Wed, 29 Aug 2018 08:06:19 GMT

←Older revision		Revision as of 08:06, 29 August 2018
Line 3:		Line 3:
	<StructureSection load='6h1u' size='340' side='right' caption='[[6h1u]], [[Resolution\|resolution]] 1.68Å' scene=''>		<StructureSection load='6h1u' size='340' side='right' caption='[[6h1u]], [[Resolution\|resolution]] 1.68Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6h1u]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H1U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H1U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6h1u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H1U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H1U FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASP:ASPARTIC+ACID'>ASP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASP:ASPARTIC+ACID'>ASP</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">glnH, Rv0411c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h1u OCA], [http://pdbe.org/6h1u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h1u RCSB], [http://www.ebi.ac.uk/pdbsum/6h1u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h1u ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h1u OCA], [http://pdbe.org/6h1u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h1u RCSB], [http://www.ebi.ac.uk/pdbsum/6h1u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h1u ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Signaling by serine/threonine phosphorylation controls diverse processes in bacteria, and identification of the stimuli that activate protein kinases is an outstanding question in the field. Recently, we showed that nutrients stimulate phosphorylation of the protein kinase G substrate GarA in Mycobacterium smegmatis and Mycobacterium tuberculosis and that the action of GarA in regulating central metabolism depends upon whether it is phosphorylated. Here we present an investigation into the mechanism by which nutrients activate PknG. Two unknown genes were identified as co-conserved and co-expressed with PknG: their products were a putative lipoprotein, GlnH, and putative transmembrane protein, GlnX. Using a genetic approach, we showed that the membrane protein GlnX is functionally linked to PknG. Furthermore, we determined that the ligand specificity of GlnH matches the amino acids that stimulate GarA phosphorylation. We determined the structure of GlnH in complex with different amino acid ligands (aspartate, glutamate, and asparagine), revealing the structural basis of ligand specificity. We propose that the amino acid concentration in the periplasm is sensed by GlnH and that protein-protein interaction allows transmission of this information across the membrane via GlnX to activate PknG. This sensory system would allow regulation of nutrient utilization in response to changes in nutrient availability. The sensor, signaling, and effector proteins are conserved throughout the Actinobacteria, including the important human pathogen Mycobacterium tuberculosis, industrial amino acid producer Corynebacterium glutamicum, and antibiotic-producing Streptomyces species.IMPORTANCE Tuberculosis (TB) kills 5,000 people every day, and the prevalence of multidrug-resistant TB is increasing in every country. The processes by which the pathogen Mycobacterium tuberculosis senses and responds to changes in its environment are attractive targets for drug development. Bacterial metabolism differs dramatically between growing and dormant cells, and these changes are known to be important in pathogenesis of TB. Here, we used genetic and biochemical approaches to identify proteins that allow M. tuberculosis to detect amino acids in its surroundings so that it can regulate its metabolism. We have also shown how individual amino acids are recognized. The findings have broader significance for other actinobacterial pathogens, such as nontuberculous mycobacteria, as well as Actinobacteria used to produce billions of dollars of amino acids and antibiotics every year.
		+
		+	An Aspartate-Specific Solute-Binding Protein Regulates Protein Kinase G Activity To Control Glutamate Metabolism in Mycobacteria.,Bhattacharyya N, Nkumama IN, Newland-Smith Z, Lin LY, Yin W, Cullen RE, Griffiths JS, Jarvis AR, Price MJ, Chong PY, Wallis R, O'Hare HM MBio. 2018 Jul 31;9(4). pii: mBio.00931-18. doi: 10.1128/mBio.00931-18. PMID:30065086<ref>PMID:30065086</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6h1u" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Myctu]]
	[[Category: Hare, H M.O]]		[[Category: Hare, H M.O]]
	[[Category: Lin, L Y]]		[[Category: Lin, L Y]]

OCA at 18:56, 1 August 2018

OCA — Wed, 01 Aug 2018 18:56:30 GMT

←Older revision		Revision as of 18:56, 1 August 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6h1u is ~~ON HOLD~~	+	==GlnH bound to Asp, Mycobacterium tuberculosis==
-		+	<StructureSection load='6h1u' size='340' side='right' caption='[[6h1u]], [[Resolution\|resolution]] 1.68Å' scene=''>
-	~~Authors~~: O'~~Hare, H~~.M.~~, Wallis, R~~., ~~Lin, L~~.Y.~~, Newland~~-~~Smith~~, Z.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6h1u]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H1U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H1U FirstGlance]. <br>
-	~~Description~~: ~~GlnH bound to Asp~~, ~~Mycobacterium tuberculosis~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASP:ASPARTIC+ACID'>ASP</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures]~~]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h1u OCA], [http://pdbe.org/6h1u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h1u RCSB], [http://www.ebi.ac.uk/pdbsum/6h1u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h1u ProSAT]</span></td></tr>
-	[[Category: O'Hare, H.M]]	+	</table>
-	[[Category: Lin, L.Y]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Hare, H M.O]]
		+	[[Category: Lin, L Y]]
		+	[[Category: Newland-Smith, Z]]
	[[Category: Wallis, R]]		[[Category: Wallis, R]]
-	[[Category: ~~Newland~~-~~Smith, Z~~]]	+	[[Category: Amino acid binding]]
		+	[[Category: Serine threonine protein kinase]]
		+	[[Category: Signaling protein]]
		+	[[Category: Solute-binding protein]]
		+	[[Category: Transport]]

OCA: Protected "6h1u" [edit=sysop:move=sysop]

OCA — Wed, 18 Jul 2018 05:55:49 GMT

Protected "6h1u" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:55, 18 July 2018

OCA: New page: '''Unreleased structure''' The entry 6h1u is ON HOLD Authors: O'Hare, H.M., Wallis, R., Lin, L.Y., Newland-Smith, Z. Description: GlnH bound to Asp, Mycobacterium tuberculosis [[Catego...

OCA — Wed, 18 Jul 2018 05:55:48 GMT

New page: '''Unreleased structure''' The entry 6h1u is ON HOLD Authors: O'Hare, H.M., Wallis, R., Lin, L.Y., Newland-Smith, Z. Description: GlnH bound to Asp, Mycobacterium tuberculosis [[Catego...

New page

'''Unreleased structure'''

The entry 6h1u is ON HOLD

Authors: O'Hare, H.M., Wallis, R., Lin, L.Y., Newland-Smith, Z.

Description: GlnH bound to Asp, Mycobacterium tuberculosis
[[Category: Unreleased Structures]]
[[Category: O'Hare, H.M]]
[[Category: Lin, L.Y]]
[[Category: Wallis, R]]
[[Category: Newland-Smith, Z]]

6h1u - Revision history

OCA at 08:06, 29 August 2018

OCA at 18:56, 1 August 2018

OCA: Protected "6h1u" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6h1u is ON HOLD Authors: O'Hare, H.M., Wallis, R., Lin, L.Y., Newland-Smith, Z. Description: GlnH bound to Asp, Mycobacterium tuberculosis [[Catego...