OCA at 07:07, 5 December 2018

OCA — Wed, 05 Dec 2018 07:07:31 GMT

←Older revision		Revision as of 07:07, 5 December 2018
Line 3:		Line 3:
	<StructureSection load='6h2n' size='340' side='right' caption='[[6h2n]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='6h2n' size='340' side='right' caption='[[6h2n]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6h2n]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H2N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H2N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6h2n]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Staan Staan]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H2N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H2N FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SA1808 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158879 STAAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h2n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h2n OCA], [http://pdbe.org/6h2n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h2n RCSB], [http://www.ebi.ac.uk/pdbsum/6h2n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h2n ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h2n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h2n OCA], [http://pdbe.org/6h2n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h2n RCSB], [http://www.ebi.ac.uk/pdbsum/6h2n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h2n ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Methicillin-resistant Staphylococcus aureus (MRSA) is a frequent cause of difficult-to-treat, often fatal infections in humans(1,2). Most humans have antibodies against S. aureus, but these are highly variable and often not protective in immunocompromised patients(3). Previous vaccine development programs have not been successful(4). A large percentage of human antibodies against S. aureus target wall teichoic acid (WTA), a ribitol-phosphate (RboP) surface polymer modified with N-acetylglucosamine (GlcNAc)(5,6). It is currently unknown whether the immune evasion capacities of MRSA are due to variation of dominant surface epitopes such as those associated with WTA. Here we show that a considerable proportion of the prominent healthcare-associated and livestock-associated MRSA clones CC5 and CC398, respectively, contain prophages that encode an alternative WTA glycosyltransferase. This enzyme, TarP, transfers GlcNAc to a different hydroxyl group of the WTA RboP than the standard enzyme TarS(7), with important consequences for immune recognition. TarP-glycosylated WTA elicits 7.5-40-fold lower levels of immunoglobulin G in mice than TarS-modified WTA. Consistent with this, human sera contained only low levels of antibodies against TarP-modified WTA. Notably, mice immunized with TarS-modified WTA were not protected against infection with tarP-expressing MRSA, indicating that TarP is crucial for the capacity of S. aureus to evade host defences. High-resolution structural analyses of TarP bound to WTA components and uridine diphosphate GlcNAc (UDP-GlcNAc) explain the mechanism of altered RboP glycosylation and form a template for targeted inhibition of TarP. Our study reveals an immune evasion strategy of S. aureus based on averting the immunogenicity of its dominant glycoantigen WTA. These results will help with the identification of invariant S. aureus vaccine antigens and may enable the development of TarP inhibitors as a new strategy for rendering MRSA susceptible to human host defences.
		+
		+	Methicillin-resistant Staphylococcus aureus alters cell wall glycosylation to evade immunity.,Gerlach D, Guo Y, De Castro C, Kim SH, Schlatterer K, Xu FF, Pereira C, Seeberger PH, Ali S, Codee J, Sirisarn W, Schulte B, Wolz C, Larsen J, Molinaro A, Lee BL, Xia G, Stehle T, Peschel A Nature. 2018 Nov;563(7733):705-709. doi: 10.1038/s41586-018-0730-x. Epub 2018 Nov, 21. PMID:30464342<ref>PMID:30464342</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6h2n" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Staan]]
	[[Category: Guo, Y]]		[[Category: Guo, Y]]
	[[Category: Stehle, T]]		[[Category: Stehle, T]]

OCA at 08:16, 26 September 2018

OCA — Wed, 26 Sep 2018 08:16:01 GMT

←Older revision		Revision as of 08:16, 26 September 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6h2n ~~is ON HOLD~~	+	==TarP-UDP-GlcNAc-Mg==
-		+	<StructureSection load='6h2n' size='340' side='right' caption='[[6h2n]], [[Resolution\|resolution]] 1.95Å' scene=''>
-	~~Authors~~: ~~Guo, Y~~.~~, Stehle, T~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6h2n]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H2N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H2N FirstGlance]. <br>
-	~~Description~~: ~~TarP~~-~~UDP~~-~~GlcNAc~~-Mg	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h2n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h2n OCA], [http://pdbe.org/6h2n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h2n RCSB], [http://www.ebi.ac.uk/pdbsum/6h2n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h2n ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
	[[Category: Guo, Y]]		[[Category: Guo, Y]]
	[[Category: Stehle, T]]		[[Category: Stehle, T]]
		+	[[Category: Gt-a fold]]
		+	[[Category: Inverting glycosyltransferase]]
		+	[[Category: Staphylococcus aureus]]
		+	[[Category: Transferase]]
		+	[[Category: Wall teichoic acid]]

OCA: Protected "6h2n" [edit=sysop:move=sysop]

OCA — Wed, 25 Jul 2018 06:03:18 GMT

Protected "6h2n" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:03, 25 July 2018

OCA: New page: '''Unreleased structure''' The entry 6h2n is ON HOLD Authors: Guo, Y., Stehle, T. Description: TarP-UDP-GlcNAc-Mg Category: Unreleased Structures Category: Guo, Y [[Category: S...

OCA — Wed, 25 Jul 2018 06:03:17 GMT

New page: '''Unreleased structure''' The entry 6h2n is ON HOLD Authors: Guo, Y., Stehle, T. Description: TarP-UDP-GlcNAc-Mg Category: Unreleased Structures Category: Guo, Y [[Category: S...

New page

'''Unreleased structure'''

The entry 6h2n is ON HOLD

Authors: Guo, Y., Stehle, T.

Description: TarP-UDP-GlcNAc-Mg
[[Category: Unreleased Structures]]
[[Category: Guo, Y]]
[[Category: Stehle, T]]

6h2n - Revision history

OCA at 07:07, 5 December 2018

OCA at 08:16, 26 September 2018

OCA: Protected "6h2n" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6h2n is ON HOLD Authors: Guo, Y., Stehle, T. Description: TarP-UDP-GlcNAc-Mg Category: Unreleased Structures Category: Guo, Y [[Category: S...