OCA at 10:17, 26 February 2020

OCA — Wed, 26 Feb 2020 10:17:52 GMT

←Older revision		Revision as of 10:17, 26 February 2020
Line 3:		Line 3:
	<StructureSection load='6h2y' size='340' side='right'caption='[[6h2y]], [[Resolution\|resolution]] 2.65Å' scene=''>		<StructureSection load='6h2y' size='340' side='right'caption='[[6h2y]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6h2y]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H2Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H2Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6h2y]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"diplokokkus_intracellularis_meningitidis"_(sic)_weichselbaum_1887 "diplokokkus intracellularis meningitidis" (sic) weichselbaum 1887] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H2Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H2Y FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=P33:3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL'>P33</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=P33:3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL'>P33</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gna1870 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=487 "Diplokokkus intracellularis meningitidis" (sic) Weichselbaum 1887])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h2y OCA], [http://pdbe.org/6h2y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h2y RCSB], [http://www.ebi.ac.uk/pdbsum/6h2y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h2y ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h2y OCA], [http://pdbe.org/6h2y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h2y RCSB], [http://www.ebi.ac.uk/pdbsum/6h2y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h2y ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The 4 component meningococcus B vaccine (4CMenB) vaccine is the first vaccine containing recombinant proteins licensed for the prevention of invasive meningococcal disease caused by meningococcal serogroup B strains. 4CMenB contains 3 main recombinant proteins, including the Neisseria meningitidis factor H binding protein (fHbp), a lipoprotein able to bind the human factor H. To date, over 1000 aa sequences of fHbp have been identified, and they can be divided into variant groups 1, 2, and 3, which are usually not crossprotective. Nevertheless, previous characterizations of a small set (n = 10) of mAbs generated in humans after 4CMenB immunization revealed 2 human Fabs (huFabs) (1A12, 1G3) with some crossreactivity for variants 1, 2, and 3. This unexpected result prompted us to examine a much larger set of human mAbs (n = 110), with the aim of better understanding the extent and nature of crossreactive anti-fHbp antibodies. In this study, we report an analysis of the human antibody response to fHbp, by the characterization of 110 huFabs collected from 3 adult vaccinees during a 6-mo study. Although the 4CMenB vaccine contains fHbp variant 1, 13 huFabs were also found to be crossreactive with variants 2 and 3. The crystal structure of the crossreactive huFab 1E6 in complex with fHbp variant 3 was determined, revealing a novel, highly conserved epitope distinct from the epitopes recognized by 1A12 or 1G3. Further, functional characterization shows that human mAb 1E6 is able to elicit rabbit, but not human, complement-mediated bactericidal activity against meningococci displaying fHbp from any of the 3 different variant groups. This functional and structural information about the human antibody response upon 4CMenB immunization contributes to further unraveling the immunogenic properties of fHbp. Knowledge gained about the epitope profile recognized by the human antibody repertoire could guide future vaccine design.-Bianchi, F., Veggi, D., Santini, L., Buricchi, F., Bartolini, E., Lo Surdo, P., Martinelli, M., Finco, O., Masignani, V., Bottomley, M. J., Maione, D., Cozzi, R. Cocrystal structure of meningococcal factor H binding protein variant 3 reveals a new crossprotective epitope recognized by human mAb 1E6.
		+
		+	Cocrystal structure of meningococcal factor H binding protein variant 3 reveals a new crossprotective epitope recognized by human mAb 1E6.,Bianchi F, Veggi D, Santini L, Buricchi F, Bartolini E, Lo Surdo P, Martinelli M, Finco O, Masignani V, Bottomley MJ, Maione D, Cozzi R FASEB J. 2019 Nov;33(11):12099-12111. doi: 10.1096/fj.201900374R. Epub 2019 Oct, 5. PMID:31442074<ref>PMID:31442074</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6h2y" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bianchi, F]]		[[Category: Bianchi, F]]

OCA at 16:27, 14 August 2019

OCA — Wed, 14 Aug 2019 16:27:33 GMT

←Older revision		Revision as of 16:27, 14 August 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6h2y ~~is ON HOLD~~	+	==human Fab 1E6 bound to fHbp variant 3 from Neisseria meningitidis serogroup B==
-		+	<StructureSection load='6h2y' size='340' side='right'caption='[[6h2y]], [[Resolution\|resolution]] 2.65Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6h2y]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H2Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H2Y FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=P33:3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL'>P33</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h2y OCA], [http://pdbe.org/6h2y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h2y RCSB], [http://www.ebi.ac.uk/pdbsum/6h2y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h2y ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bianchi, F]]
		+	[[Category: Bottomley, M J]]
		+	[[Category: Cozzi, R]]
		+	[[Category: Malito, E]]
		+	[[Category: Veggi, D]]
		+	[[Category: Antigen-antibody complex]]
		+	[[Category: Fhbp]]
		+	[[Category: Human fab]]
		+	[[Category: Immune system]]
		+	[[Category: Neisseria]]

OCA: Protected "6h2y" [edit=sysop:move=sysop]

OCA — Wed, 25 Jul 2018 06:03:53 GMT

Protected "6h2y" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:03, 25 July 2018

OCA: New page: '''Unreleased structure''' The entry 6h2y is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 25 Jul 2018 06:03:52 GMT

New page: '''Unreleased structure''' The entry 6h2y is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6h2y is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6h2y - Revision history

OCA at 10:17, 26 February 2020

OCA at 16:27, 14 August 2019

OCA: Protected "6h2y" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6h2y is ON HOLD Authors: Description: Category: Unreleased Structures