6h3n - Revision history

OCA at 01:13, 11 April 2020

OCA — Sat, 11 Apr 2020 01:13:14 GMT

←Older revision		Revision as of 01:13, 11 April 2020
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	<SX load='6h3n' size='340' side='right' viewer='molstar' caption='[[6h3n]], [[Resolution\|resolution]] 3.25Å' scene=''>		<SX load='6h3n' size='340' side='right' viewer='molstar' caption='[[6h3n]], [[Resolution\|resolution]] 3.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6h3n]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H3N OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6H3N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6h3n]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H3N OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6H3N FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">vgrG1, PA0091 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">vgrG1, PA0091 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6h3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h3n OCA], [http://pdbe.org/6h3n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h3n RCSB], [http://www.ebi.ac.uk/pdbsum/6h3n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h3n ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6h3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h3n OCA], [http://pdbe.org/6h3n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h3n RCSB], [http://www.ebi.ac.uk/pdbsum/6h3n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h3n ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 21:36, 6 March 2020

OCA — Fri, 06 Mar 2020 21:36:36 GMT

←Older revision		Revision as of 21:36, 6 March 2020
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	==Structure of VgrG1 in the Type VI secretion VgrG1-Tse6-EF-Tu complex embedded in lipid nanodiscs==		==Structure of VgrG1 in the Type VI secretion VgrG1-Tse6-EF-Tu complex embedded in lipid nanodiscs==
-	<~~StructureSection~~ load='6h3n' size='340' side='right' caption='[[6h3n]], [[Resolution\|resolution]] 3.25Å' scene=''>	+	<SX load='6h3n' size='340' side='right' viewer='molstar' caption='[[6h3n]], [[Resolution\|resolution]] 3.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6h3n]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H3N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H3N FirstGlance]. <br>		<table><tr><td colspan='2'>[[6h3n]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H3N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H3N FirstGlance]. <br>
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	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
		+	[[Category: Large Structures]]
	[[Category: Pseae]]		[[Category: Pseae]]
	[[Category: Quentin, D]]		[[Category: Quentin, D]]

OCA at 08:57, 3 October 2018

OCA — Wed, 03 Oct 2018 08:57:07 GMT

←Older revision		Revision as of 08:57, 3 October 2018
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	<StructureSection load='6h3n' size='340' side='right' caption='[[6h3n]], [[Resolution\|resolution]] 3.25Å' scene=''>		<StructureSection load='6h3n' size='340' side='right' caption='[[6h3n]], [[Resolution\|resolution]] 3.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6h3n]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H3N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H3N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6h3n]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H3N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H3N FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h3n OCA], [http://pdbe.org/6h3n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h3n RCSB], [http://www.ebi.ac.uk/pdbsum/6h3n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h3n ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">vgrG1, PA0091 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h3n OCA], [http://pdbe.org/6h3n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h3n RCSB], [http://www.ebi.ac.uk/pdbsum/6h3n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h3n ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Pseae]]
	[[Category: Quentin, D]]		[[Category: Quentin, D]]
	[[Category: Raunser, S]]		[[Category: Raunser, S]]

OCA at 07:41, 12 September 2018

OCA — Wed, 12 Sep 2018 07:41:25 GMT

←Older revision		Revision as of 07:41, 12 September 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6h3n is ~~ON HOLD until Paper~~ Publication	+	==Structure of VgrG1 in the Type VI secretion VgrG1-Tse6-EF-Tu complex embedded in lipid nanodiscs==
		+	<StructureSection load='6h3n' size='340' side='right' caption='[[6h3n]], [[Resolution\|resolution]] 3.25Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6h3n]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H3N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H3N FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h3n OCA], [http://pdbe.org/6h3n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h3n RCSB], [http://www.ebi.ac.uk/pdbsum/6h3n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h3n ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The type VI secretion system (T6SS) primarily functions to mediate antagonistic interactions between contacting bacterial cells, but also mediates interactions with eukaryotic hosts. This molecular machine secretes antibacterial effector proteins by undergoing cycles of extension and contraction; however, how effectors are loaded into the T6SS and subsequently delivered to target bacteria remains poorly understood. Here, using electron cryomicroscopy, we analysed the structures of the Pseudomonas aeruginosa effector Tse6 loaded onto the T6SS spike protein VgrG1 in solution and embedded in lipid nanodiscs. In the absence of membranes, Tse6 stability requires the chaperone EagT6, two dimers of which interact with the hydrophobic transmembrane domains of Tse6. EagT6 is not directly involved in Tse6 delivery but is crucial for its loading onto VgrG1. VgrG1-loaded Tse6 spontaneously enters membranes and its toxin domain translocates across a lipid bilayer, indicating that effector delivery by the T6SS does not require puncturing of the target cell inner membrane by VgrG1. Eag chaperone family members from diverse Proteobacteria are often encoded adjacent to putative toxins with predicted transmembrane domains and we therefore anticipate that our findings will be generalizable to numerous T6SS-exported membrane-associated effectors.

-	~~Authors: Quentin~~, D., ~~Raunser~~, S.	+	Mechanism of loading and translocation of type VI secretion system effector Tse6.,Quentin D, Ahmad S, Shanthamoorthy P, Mougous JD, Whitney JC, Raunser S Nat Microbiol. 2018 Sep 3. pii: 10.1038/s41564-018-0238-z. doi:, 10.1038/s41564-018-0238-z. PMID:30177742<ref>PMID:30177742</ref>

-	~~Description: Structure~~ of ~~VgrG1 in~~ the ~~Type VI secretion VgrG1~~-~~Tse6~~-~~EF-Tu complex embedded in lipid nanodiscs~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 6h3n" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Quentin, D]]		[[Category: Quentin, D]]
	[[Category: Raunser, S]]		[[Category: Raunser, S]]
		+	[[Category: Bacterial type vi effector complex]]
		+	[[Category: Toxin]]
		+	[[Category: Tse6-loaded vgrg1 complex]]

OCA at 04:51, 22 August 2018

OCA — Wed, 22 Aug 2018 04:51:30 GMT

←Older revision		Revision as of 04:51, 22 August 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6h3n is ON HOLD	+	The entry 6h3n is ON HOLD until Paper Publication

	Authors: Quentin, D., Raunser, S.		Authors: Quentin, D., Raunser, S.

OCA: Protected "6h3n" [edit=sysop:move=sysop]

OCA — Wed, 25 Jul 2018 06:06:15 GMT

Protected "6h3n" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:06, 25 July 2018

OCA: New page: '''Unreleased structure''' The entry 6h3n is ON HOLD Authors: Quentin, D., Raunser, S. Description: Structure of VgrG1 in the Type VI secretion VgrG1-Tse6-EF-Tu complex embedded in lip...

OCA — Wed, 25 Jul 2018 06:06:14 GMT

New page: '''Unreleased structure''' The entry 6h3n is ON HOLD Authors: Quentin, D., Raunser, S. Description: Structure of VgrG1 in the Type VI secretion VgrG1-Tse6-EF-Tu complex embedded in lip...

New page

'''Unreleased structure'''

The entry 6h3n is ON HOLD

Authors: Quentin, D., Raunser, S.

Description: Structure of VgrG1 in the Type VI secretion VgrG1-Tse6-EF-Tu complex embedded in lipid nanodiscs
[[Category: Unreleased Structures]]
[[Category: Quentin, D]]
[[Category: Raunser, S]]