OCA at 12:09, 16 January 2019

OCA — Wed, 16 Jan 2019 12:09:11 GMT

←Older revision		Revision as of 12:09, 16 January 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6h40 is ~~ON HOLD until Paper~~ Publication	+	==High resolution structure of MeT1 from Mycobacterium hassiacum in complex with 3-methoxy-1,2-propanediol.==
		+	<StructureSection load='6h40' size='340' side='right' caption='[[6h40]], [[Resolution\|resolution]] 1.05Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6h40]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H40 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H40 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FW5:(2~{S})-3-methoxypropane-1,2-diol'>FW5</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6g7d\|6g7d]], [[6g80\|6g80]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h40 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h40 OCA], [http://pdbe.org/6h40 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h40 RCSB], [http://www.ebi.ac.uk/pdbsum/6h40 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h40 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mycobacteria are a wide group of organisms that includes strict pathogens, such as Mycobacterium tuberculosis, as well as environmental species known as nontuberculous mycobacteria (NTM), some of which-namely Mycobacterium avium-are important opportunistic pathogens. In addition to a distinctive cell envelope mediating critical interactions with the host immune system and largely responsible for their formidable resistance to antimicrobials, mycobacteria synthesize rare intracellular polymethylated polysaccharides implicated in the modulation of fatty acid metabolism, thus critical players in cell envelope assembly. These are the 6-O-methylglucose lipopolysaccharides (MGLP) ubiquitously detected across the Mycobacterium genus, and the 3-O-methylmannose polysaccharides (MMP) identified only in NTM. The polymethylated nature of these polysaccharides renders the intervening methyltransferases essential for their optimal function. Although the knowledge of MGLP biogenesis is greater than that of MMP biosynthesis, the methyltransferases of both pathways remain uncharacterized. Here, we report the identification and characterization of a unique S-adenosyl-l-methionine-dependent sugar 1-O-methyltransferase (MeT1) from Mycobacterium hassiacum that specifically blocks the 1-OH position of 3,3'-di-O-methyl-4alpha-mannobiose, a probable early precursor of MMP, which we chemically synthesized. The high-resolution 3D structure of MeT1 in complex with its exhausted cofactor, S-adenosyl-l-homocysteine, together with mutagenesis studies and molecular docking simulations, unveiled the enzyme's reaction mechanism. The functional and structural properties of this unique sugar methyltransferase further our knowledge of MMP biosynthesis and provide important tools to dissect the role of MMP in NTM physiology and resilience.

-	~~Authors: Pereira, P.J.B~~., Ripoll-Rozada, J.	+	Biosynthesis of mycobacterial methylmannose polysaccharides requires a unique 1-O-methyltransferase specific for 3-O-methylated mannosides.,Ripoll-Rozada J, Costa M, Manso JA, Maranha A, Miranda V, Sequeira A, Ventura MR, Macedo-Ribeiro S, Pereira PJB, Empadinhas N Proc Natl Acad Sci U S A. 2019 Jan 15;116(3):835-844. doi:, 10.1073/pnas.1813450116. Epub 2019 Jan 3. PMID:30606802<ref>PMID:30606802</ref>

-	~~Description~~: ~~Mycobacterium hassiacum methyltransferase~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6h40" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Pereira, P J.B]]
	[[Category: Ripoll-Rozada, J]]		[[Category: Ripoll-Rozada, J]]
-	[[Category: ~~Pereira, P.J.B~~]]	+	[[Category: 3-o-methyltransferase]]
		+	[[Category: Transferase]]

OCA at 20:02, 9 August 2018

OCA — Thu, 09 Aug 2018 20:02:55 GMT

←Older revision		Revision as of 20:02, 9 August 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6h40 is ON HOLD	+	The entry 6h40 is ON HOLD until Paper Publication

	Authors: Pereira, P.J.B., Ripoll-Rozada, J.		Authors: Pereira, P.J.B., Ripoll-Rozada, J.
Line 7:		Line 7:
	Description: Mycobacterium hassiacum methyltransferase		Description: Mycobacterium hassiacum methyltransferase
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Pereira, P.J.B]]
	[[Category: Ripoll-Rozada, J]]		[[Category: Ripoll-Rozada, J]]
		+	[[Category: Pereira, P.J.B]]

OCA: Protected "6h40" [edit=sysop:move=sysop]

OCA — Wed, 25 Jul 2018 06:06:55 GMT

Protected "6h40" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:06, 25 July 2018

OCA: New page: '''Unreleased structure''' The entry 6h40 is ON HOLD Authors: Pereira, P.J.B., Ripoll-Rozada, J. Description: Mycobacterium hassiacum methyltransferase [[Category: Unreleased Structure...

OCA — Wed, 25 Jul 2018 06:06:54 GMT

New page: '''Unreleased structure''' The entry 6h40 is ON HOLD Authors: Pereira, P.J.B., Ripoll-Rozada, J. Description: Mycobacterium hassiacum methyltransferase [[Category: Unreleased Structure...

New page

'''Unreleased structure'''

The entry 6h40 is ON HOLD

Authors: Pereira, P.J.B., Ripoll-Rozada, J.

Description: Mycobacterium hassiacum methyltransferase
[[Category: Unreleased Structures]]
[[Category: Pereira, P.J.B]]
[[Category: Ripoll-Rozada, J]]

6h40 - Revision history

OCA at 12:09, 16 January 2019

OCA at 20:02, 9 August 2018

OCA: Protected "6h40" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6h40 is ON HOLD Authors: Pereira, P.J.B., Ripoll-Rozada, J. Description: Mycobacterium hassiacum methyltransferase [[Category: Unreleased Structure...