6h5q - Revision history

OCA at 01:14, 11 April 2020

OCA — Sat, 11 Apr 2020 01:14:18 GMT

←Older revision		Revision as of 01:14, 11 April 2020
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	<SX load='6h5q' size='340' side='right' viewer='molstar' caption='[[6h5q]], [[Resolution\|resolution]] 3.30Å' scene=''>		<SX load='6h5q' size='340' side='right' viewer='molstar' caption='[[6h5q]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6h5q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cell-associated_subacute_sclerosing_panencephalitis Cell-associated subacute sclerosing panencephalitis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H5Q OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6H5Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6h5q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cell-associated_subacute_sclerosing_panencephalitis Cell-associated subacute sclerosing panencephalitis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H5Q OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6H5Q FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6h5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h5q OCA], [http://pdbe.org/6h5q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h5q RCSB], [http://www.ebi.ac.uk/pdbsum/6h5q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h5q ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6h5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h5q OCA], [http://pdbe.org/6h5q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h5q RCSB], [http://www.ebi.ac.uk/pdbsum/6h5q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h5q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 21:36, 6 March 2020

OCA — Fri, 06 Mar 2020 21:36:58 GMT

←Older revision		Revision as of 21:36, 6 March 2020
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	==Cryo-EM structure of in vitro assembled Measles virus N into nucleocapsid-like particles (NCLPs) bound to polyA RNA hexamers.==		==Cryo-EM structure of in vitro assembled Measles virus N into nucleocapsid-like particles (NCLPs) bound to polyA RNA hexamers.==
-	<~~StructureSection~~ load='6h5q' size='340' side='right'caption='[[6h5q]], [[Resolution\|resolution]] 3.30Å' scene=''>	+	<SX load='6h5q' size='340' side='right' viewer='molstar' caption='[[6h5q]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6h5q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cell-associated_subacute_sclerosing_panencephalitis Cell-associated subacute sclerosing panencephalitis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H5Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H5Q FirstGlance]. <br>		<table><tr><td colspan='2'>[[6h5q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cell-associated_subacute_sclerosing_panencephalitis Cell-associated subacute sclerosing panencephalitis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H5Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H5Q FirstGlance]. <br>
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	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Cell-associated subacute sclerosing panencephalitis]]		[[Category: Cell-associated subacute sclerosing panencephalitis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 06:52, 31 July 2019

OCA — Wed, 31 Jul 2019 06:52:29 GMT

←Older revision		Revision as of 06:52, 31 July 2019
Line 3:		Line 3:
	<StructureSection load='6h5q' size='340' side='right'caption='[[6h5q]], [[Resolution\|resolution]] 3.30Å' scene=''>		<StructureSection load='6h5q' size='340' side='right'caption='[[6h5q]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6h5q]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H5Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H5Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6h5q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cell-associated_subacute_sclerosing_panencephalitis Cell-associated subacute sclerosing panencephalitis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H5Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H5Q FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h5q OCA], [http://pdbe.org/6h5q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h5q RCSB], [http://www.ebi.ac.uk/pdbsum/6h5q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h5q ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h5q OCA], [http://pdbe.org/6h5q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h5q RCSB], [http://www.ebi.ac.uk/pdbsum/6h5q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h5q ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Cell-associated subacute sclerosing panencephalitis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Blackledge, M]]		[[Category: Blackledge, M]]

OCA at 12:01, 13 March 2019

OCA — Wed, 13 Mar 2019 12:01:51 GMT

←Older revision		Revision as of 12:01, 13 March 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6h5q is ~~ON HOLD until Paper~~ Publication	+	==Cryo-EM structure of in vitro assembled Measles virus N into nucleocapsid-like particles (NCLPs) bound to polyA RNA hexamers.==
		+	<StructureSection load='6h5q' size='340' side='right'caption='[[6h5q]], [[Resolution\|resolution]] 3.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6h5q]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H5Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H5Q FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h5q OCA], [http://pdbe.org/6h5q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h5q RCSB], [http://www.ebi.ac.uk/pdbsum/6h5q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h5q ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/B8PZP0_9MONO B8PZP0_9MONO]] Encapsidates the genome, protecting it from nucleases.[RuleBase:RU361245]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Assembly of paramyxoviral nucleocapsids on the RNA genome is an essential step in the viral cycle. The structural basis of this process has remained obscure due to the inability to control encapsidation. We used a recently developed approach to assemble measles virus nucleocapsid-like particles on specific sequences of RNA hexamers (poly-Adenine and viral genomic 5') in vitro, and determined their cryoelectron microscopy maps to 3.3-A resolution. The structures unambiguously determine 5' and 3' binding sites and thereby the binding-register of viral genomic RNA within nucleocapsids. This observation reveals that the 3' end of the genome is largely exposed in fully assembled measles nucleocapsids. In particular, the final three nucleotides of the genome are rendered accessible to the RNA-dependent RNA polymerase complex, possibly enabling efficient RNA processing. The structures also reveal local and global conformational changes in the nucleoprotein upon assembly, in particular involving helix alpha6 and helix alpha13 that form edges of the RNA binding groove. Disorder is observed in the bound RNA, localized at one of the two backbone conformational switch sites. The high-resolution structure allowed us to identify putative nucleobase interaction sites in the RNA-binding groove, whose impact on assembly kinetics was measured using real-time NMR. Mutation of one of these sites, R195, whose sidechain stabilizes both backbone and base of a bound nucleic acid, is thereby shown to be essential for nucleocapsid-like particle assembly.

-	~~Authors:~~ Desfosses, A., Milles, S., ~~Ringkjobing~~ Jensen~~, M.~~, Guseva, S., Colletier~~, J.~~, Maurin, D., Schoehn, G., Gutsche, I., Ruigrok~~, R.~~, Blackledge, M.	+	Assembly and cryo-EM structures of RNA-specific measles virus nucleocapsids provide mechanistic insight into paramyxoviral replication.,Desfosses A, Milles S, Jensen MR, Guseva S, Colletier JP, Maurin D, Schoehn G, Gutsche I, Ruigrok RWH, Blackledge M Proc Natl Acad Sci U S A. 2019 Feb 20. pii: 1816417116. doi:, 10.1073/pnas.1816417116. PMID:30787192<ref>PMID:30787192</ref>

-	~~Description: Cryo-EM structure~~ of ~~in vitro assembled Measles virus N into nucleocapsid-like particles (NCLPs) bound to polyA RNA hexamers~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6h5q" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Blackledge, M]]		[[Category: Blackledge, M]]
-	[[Category: Maurin, D]]
-	[[Category: Gutsche, I]]
-	[[Category: Desfosses, A]]
-	[[Category: Ruigrok, R]]
	[[Category: Colletier, J]]		[[Category: Colletier, J]]
		+	[[Category: Desfosses, A]]
	[[Category: Guseva, S]]		[[Category: Guseva, S]]
-	[[Category: ~~Schoehn~~, G]]	+	[[Category: Gutsche, I]]
-	[[Category: ~~Ringkjobing~~ Jensen, M]]	+	[[Category: Jensen, M Ringkjobing]]
		+	[[Category: Maurin, D]]
	[[Category: Milles, S]]		[[Category: Milles, S]]
		+	[[Category: Ruigrok, R]]
		+	[[Category: Schoehn, G]]
		+	[[Category: Helical]]
		+	[[Category: Measles]]
		+	[[Category: Nucleocapsid]]
		+	[[Category: Rna]]
		+	[[Category: Viral protein]]

OCA at 20:03, 9 August 2018

OCA — Thu, 09 Aug 2018 20:03:30 GMT

←Older revision		Revision as of 20:03, 9 August 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6h5q is ON HOLD	+	The entry 6h5q is ON HOLD until Paper Publication

	Authors: Desfosses, A., Milles, S., Ringkjobing Jensen, M., Guseva, S., Colletier, J., Maurin, D., Schoehn, G., Gutsche, I., Ruigrok, R., Blackledge, M.		Authors: Desfosses, A., Milles, S., Ringkjobing Jensen, M., Guseva, S., Colletier, J., Maurin, D., Schoehn, G., Gutsche, I., Ruigrok, R., Blackledge, M.
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Blackledge, M]]		[[Category: Blackledge, M]]
-	[[Category: Guseva, S]]
-	[[Category: Schoehn, G]]
	[[Category: Maurin, D]]		[[Category: Maurin, D]]
	[[Category: Gutsche, I]]		[[Category: Gutsche, I]]
	[[Category: Desfosses, A]]		[[Category: Desfosses, A]]
	[[Category: Ruigrok, R]]		[[Category: Ruigrok, R]]
-	[[Category: Ringkjobing Jensen, M]]
	[[Category: Colletier, J]]		[[Category: Colletier, J]]
		+	[[Category: Guseva, S]]
		+	[[Category: Schoehn, G]]
		+	[[Category: Ringkjobing Jensen, M]]
	[[Category: Milles, S]]		[[Category: Milles, S]]

OCA: Protected "6h5q" [edit=sysop:move=sysop]

OCA — Wed, 01 Aug 2018 17:18:09 GMT

Protected "6h5q" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:18, 1 August 2018

OCA: New page: '''Unreleased structure''' The entry 6h5q is ON HOLD Authors: Desfosses, A., Milles, S., Ringkjobing Jensen, M., Guseva, S., Colletier, J., Maurin, D., Schoehn, G., Gutsche, I., Ruigrok...

OCA — Wed, 01 Aug 2018 17:18:08 GMT

New page: '''Unreleased structure''' The entry 6h5q is ON HOLD Authors: Desfosses, A., Milles, S., Ringkjobing Jensen, M., Guseva, S., Colletier, J., Maurin, D., Schoehn, G., Gutsche, I., Ruigrok...

New page

'''Unreleased structure'''

The entry 6h5q is ON HOLD

Authors: Desfosses, A., Milles, S., Ringkjobing Jensen, M., Guseva, S., Colletier, J., Maurin, D., Schoehn, G., Gutsche, I., Ruigrok, R., Blackledge, M.

Description: Cryo-EM structure of in vitro assembled Measles virus N into nucleocapsid-like particles (NCLPs) bound to polyA RNA hexamers.
[[Category: Unreleased Structures]]
[[Category: Blackledge, M]]
[[Category: Guseva, S]]
[[Category: Schoehn, G]]
[[Category: Maurin, D]]
[[Category: Gutsche, I]]
[[Category: Desfosses, A]]
[[Category: Ruigrok, R]]
[[Category: Ringkjobing Jensen, M]]
[[Category: Colletier, J]]
[[Category: Milles, S]]