6h5t - Revision history

OCA at 13:34, 10 May 2019

OCA — Fri, 10 May 2019 13:34:08 GMT

←Older revision		Revision as of 13:34, 10 May 2019
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	==Intersectin SH3A short isoform==		==Intersectin SH3A short isoform==
-	<StructureSection load='6h5t' size='340' side='right' caption='[[6h5t]], [[Resolution\|resolution]] 1.69Å' scene=''>	+	<StructureSection load='6h5t' size='340' side='right'caption='[[6h5t]], [[Resolution\|resolution]] 1.69Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6h5t]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H5T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H5T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6h5t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H5T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H5T FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7PG:2,5,8,11,14,17,20,23-OCTAOXAPENTACOSAN-25-OL'>7PG</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7PG:2,5,8,11,14,17,20,23-OCTAOXAPENTACOSAN-25-OL'>7PG</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ITSN1, ITSN, SH3D1A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h5t OCA], [http://pdbe.org/6h5t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h5t RCSB], [http://www.ebi.ac.uk/pdbsum/6h5t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h5t ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h5t OCA], [http://pdbe.org/6h5t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h5t RCSB], [http://www.ebi.ac.uk/pdbsum/6h5t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h5t ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/ITSN1_HUMAN ITSN1_HUMAN]] Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2. Isoform 1 could be involved in brain-specific synaptic vesicle recycling. Inhibits ARHGAP31 activity toward RAC1.<ref>PMID:11744688</ref> <ref>PMID:22648170</ref>		[[http://www.uniprot.org/uniprot/ITSN1_HUMAN ITSN1_HUMAN]] Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2. Isoform 1 could be involved in brain-specific synaptic vesicle recycling. Inhibits ARHGAP31 activity toward RAC1.<ref>PMID:11744688</ref> <ref>PMID:22648170</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The scaffolding protein intersectin 1 plays important roles in clathrin-mediated endocytosis and in the replenishment of release-ready synaptic vesicles (SV). Two splice variants of intersectin's SH3A domain are expressed in the brain, and association of the neuron-specific variant with synapsin I has been shown to enable sustained neurotransmission and to be regulated by an adjacent C-terminal motif. Here, we demonstrate that the ubiquitously expressed short SH3A variant of intersectin 1 interacts with an N-terminal intramolecular sequence that operates synergistically with the C-terminal motif. NMR spectroscopic investigations show that the five-amino acid insertion into the beta strand 2 of the neuronal SH3A variant introduces conformational plasticity incompatible with binding of the N-terminal sequence. The difference in the autoregulatory mechanism of the domain's variants differentially affects its synaptic binding partners, thereby establishing alternative splicing in conjunction with autoinhibitory motif variation as a mechanism to regulate protein interaction networks.
		+
		+	Exon Inclusion Modulates Conformational Plasticity and Autoinhibition of the Intersectin 1 SH3A Domain.,Gerth F, Japel M, Sticht J, Kuropka B, Schmitt XJ, Driller JH, Loll B, Wahl MC, Pagel K, Haucke V, Freund C Structure. 2019 Apr 9. pii: S0969-2126(19)30116-9. doi:, 10.1016/j.str.2019.03.020. PMID:31031201<ref>PMID:31031201</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6h5t" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Driller, J H]]		[[Category: Driller, J H]]
	[[Category: Freund, C]]		[[Category: Freund, C]]

OCA at 15:21, 27 February 2019

OCA — Wed, 27 Feb 2019 15:21:15 GMT

←Older revision		Revision as of 15:21, 27 February 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6h5t is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Intersectin SH3A short isoform==
-		+	<StructureSection load='6h5t' size='340' side='right' caption='[[6h5t]], [[Resolution\|resolution]] 1.69Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6h5t]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H5T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H5T FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7PG:2,5,8,11,14,17,20,23-OCTAOXAPENTACOSAN-25-OL'>7PG</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h5t OCA], [http://pdbe.org/6h5t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h5t RCSB], [http://www.ebi.ac.uk/pdbsum/6h5t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h5t ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ITSN1_HUMAN ITSN1_HUMAN]] Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2. Isoform 1 could be involved in brain-specific synaptic vesicle recycling. Inhibits ARHGAP31 activity toward RAC1.<ref>PMID:11744688</ref> <ref>PMID:22648170</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Driller, J H]]
		+	[[Category: Freund, C]]
		+	[[Category: Gerth, F]]
		+	[[Category: Loll, B]]
		+	[[Category: Wahl, M C]]
		+	[[Category: Endocytosis]]
		+	[[Category: Intersectin 1]]
		+	[[Category: Sh3 domain]]
		+	[[Category: Splice isoform]]

OCA at 20:03, 9 August 2018

OCA — Thu, 09 Aug 2018 20:03:46 GMT

←Older revision		Revision as of 20:03, 9 August 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6h5t is ON HOLD	+	The entry 6h5t is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6h5t" [edit=sysop:move=sysop]

OCA — Wed, 01 Aug 2018 17:18:32 GMT

Protected "6h5t" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:18, 1 August 2018

OCA: New page: '''Unreleased structure''' The entry 6h5t is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 01 Aug 2018 17:18:31 GMT

New page: '''Unreleased structure''' The entry 6h5t is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6h5t is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]